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Unique exposure system for the whole body inhalation experiments with small animals
Večeřa, Zbyněk ; Mikuška, Pavel ; Moravec, Pavel ; Smolík, Jiří
The especially inhalation chamber is constructed for chronic exposure studies of small animals to nanoparticles under strictly controlled conditions in frame of project „Study of transport of inhalated nano-sized particles (Pb, Cd) and their allocation in organs“. The inhalation chamber builds from a glass and stainless steel and makes possible long-term inhalation procedures (up to 60 days, 24 hours/day) with up to four discrete groups of mice population (up to 60 individuals each) or of rats population (up to 20 individuals each) under controlled illumination, temperature, relative humidity, doses (0.5 x 106 - 5 x 106 particles/cm3) and size (10-60 nm) of nano-sized particles which are synthesized via aerosol route in a hot wall tube flow reactor using metal organic precursors.
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Sensor based on Förster resonance energy transfer with quantum dot
Lišková, Marcela ; Datinská, Vladimíra ; Klepárník, Karel ; Foret, František
Förster resonance energy transfers (FRET) are based on the nonradiative transfer of energy between a donor and acceptor dye in the distance 1-10 nm. With respect to this, quantum dots (QDs) with a high absorbance can serve as suitable donors of energy in FRET. Here, we present architectures of two sensors based on QD conjugates useful in imaging and diagnostics of cancer. One sensor, based on an oligonucleotide conjugate, can be used for the detection of complementary oligonucletide chains. The other was designed for the detection of enzymes inside cells.
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