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Stanovení hydroxykyselin včetně jejich nízko- a vysokomolekulárních derivátů
Lysáková, Klára ; Mravcová, Ludmila (oponent) ; Čáslavský, Josef (vedoucí práce)
Tato bakalářská práce se zabývá problematikou stanovení hydroxykyselin a jejich nízko- a vysokomolekulárních polymerů. Teoretická část pojednává o jejich vlastnostech, výrobě a využití. Polymery těchto látek jsou hojně využívané jako biodegradabilní plasty a jako vstřebatelné šicí materiály. Experimentální část je zaměřena na stanovení celkového složení a dále pak složení a množství nečistot vybraných vzorků ethyllaktátu, laktidů a polylaktidu. Analýza probíhala pomocí plynové chromatografie (GC) a pomocí tandemových technik, ve kterých bylo využito spojení plynové chromatografie a hmotnostní spektrometrie (GC/MS), popřípadě ještě spojení Head-Space GC/MS analýzy.
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Optimization of methods for protein analysis released from thermosensitive hydrogel
Lysáková, Klára ; Brtníková, Jana (oponent) ; Vojtová, Lucy (vedoucí práce)
Proposed diploma thesis is focused on the release evaluation methods of both model proteins (albumin and lysozyme) and tissue healing protein (stable fibroblast growth factor 2; FGF2-stab) from the “smart” hydrogel carrier based on the biodegradable thermosensitive PLGA-PEG-PLGA triblock copolymer. In the theoretical part, a brief overview of thermosensitive polymers, their properties, structure and utilization of aforementioned proteins is described. Moreover, types of interactions that may occur between proteins and the polymeric carrier including drug release models are mentioned. In the experimental part, the structure of PLGA-PEG-PLGA triblock copolymer was characterized by the gel permeation chromatography and 1H NMR while its visco-elastic properties including sol-gel transition were evaluated by the rheological analysis. Thesis goal was targeted to better understand the release of newly-patented FGF2-stab protein from injectable PLGA-PEG-PLGA hydrogel formed at physiological temperature. The amount of released FGF2-stab was measured by the UV-VIS spectrophotometer in the presence of a Bradford reagent that binds proteins resulting in shifting their absorption maxima from 280 nm to 595 nm. For the comparison, SDS-page electrophoresis, dividing protein by molecular weight, has been used. It has been find out, that model proteins, which were in different size but predominantly non-polar on their surface exhibited two-stage release dependent on both the diffusion and polymer degradation, whereas, FGF2-stab (25 kg.mol-1) showed controlled one-stage first-order release from the PLGA-PEG-PLGA hydrogel forced only by diffusion, since it is predominantly a polar molecule located probably at the hydrogel micelle surface and not in their core. These results are very important to tune the protein release from hydrogel carriers to meet their certain application, in this case specifically in controlled tissue regeneration.
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Optimization of methods for protein analysis released from thermosensitive hydrogel
Lysáková, Klára ; Brtníková, Jana (oponent) ; Vojtová, Lucy (vedoucí práce)
Proposed diploma thesis is focused on the release evaluation methods of both model proteins (albumin and lysozyme) and tissue healing protein (stable fibroblast growth factor 2; FGF2-stab) from the “smart” hydrogel carrier based on the biodegradable thermosensitive PLGA-PEG-PLGA triblock copolymer. In the theoretical part, a brief overview of thermosensitive polymers, their properties, structure and utilization of aforementioned proteins is described. Moreover, types of interactions that may occur between proteins and the polymeric carrier including drug release models are mentioned. In the experimental part, the structure of PLGA-PEG-PLGA triblock copolymer was characterized by the gel permeation chromatography and 1H NMR while its visco-elastic properties including sol-gel transition were evaluated by the rheological analysis. Thesis goal was targeted to better understand the release of newly-patented FGF2-stab protein from injectable PLGA-PEG-PLGA hydrogel formed at physiological temperature. The amount of released FGF2-stab was measured by the UV-VIS spectrophotometer in the presence of a Bradford reagent that binds proteins resulting in shifting their absorption maxima from 280 nm to 595 nm. For the comparison, SDS-page electrophoresis, dividing protein by molecular weight, has been used. It has been find out, that model proteins, which were in different size but predominantly non-polar on their surface exhibited two-stage release dependent on both the diffusion and polymer degradation, whereas, FGF2-stab (25 kg.mol-1) showed controlled one-stage first-order release from the PLGA-PEG-PLGA hydrogel forced only by diffusion, since it is predominantly a polar molecule located probably at the hydrogel micelle surface and not in their core. These results are very important to tune the protein release from hydrogel carriers to meet their certain application, in this case specifically in controlled tissue regeneration.
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Stanovení hydroxykyselin včetně jejich nízko- a vysokomolekulárních derivátů
Lysáková, Klára ; Mravcová, Ludmila (oponent) ; Čáslavský, Josef (vedoucí práce)
Tato bakalářská práce se zabývá problematikou stanovení hydroxykyselin a jejich nízko- a vysokomolekulárních polymerů. Teoretická část pojednává o jejich vlastnostech, výrobě a využití. Polymery těchto látek jsou hojně využívané jako biodegradabilní plasty a jako vstřebatelné šicí materiály. Experimentální část je zaměřena na stanovení celkového složení a dále pak složení a množství nečistot vybraných vzorků ethyllaktátu, laktidů a polylaktidu. Analýza probíhala pomocí plynové chromatografie (GC) a pomocí tandemových technik, ve kterých bylo využito spojení plynové chromatografie a hmotnostní spektrometrie (GC/MS), popřípadě ještě spojení Head-Space GC/MS analýzy.
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