National Repository of Grey Literature 4 records found  Search took 0.00 seconds. 
Kinetoplastida of birds and bloodsucking Diptera: molecular characterization and life cycles
Zídková, Lenka
We studied biodiversity of avian trypanosomes. We used newly obtained isolates of trypanosomatids from ornitophilic bloodsucking diptera (81) and from avian hosts (145). To investigate relationships among isolates we employed RAPD method and sequence analyses of SSU rRNA, eventually gGAPDH, 5S RNA or SL RNA. Additionally we used morphological characters; cell lengths and kinetoplast widths. All isolates obtained from biting midges belonged to monogenetic kinetoplastids. We described new species and a new genus Sergeia podlipaevi isolated from Culicoides (Oecacta) festivipennis a C. (O.) truncorum. We successfully infected with this species laboratory bred biting midge of different subgenus Culicoides (Monoculicoides) nubeculosus. We described isolate from Culicoides truncorum as new species Herpetomonas trimorpha. Its sister species was H. ztiplika described previously also from biting midges. We performed the most extensive study of biodiversity of avian trypanosomes based on comparison of wide spectrum of isolates obtained from different hosts. We confirmed that avian trypanosomes are polyphyletic and form three independent lineages within the genus Trypanosoma. We identified at least 11 species of avian trypanosomes. We found that ornitophilic mosquitoes belong among important vectors of avian...
Sand flies as hosts of monoxenous and dixenous trypanosomatids
Vondráček, Oldřich ; Votýpka, Jan (advisor) ; Zídková, Lenka (referee)
Sandflies (Diptera: Psychodidae: Phlebotominae) are blood-sucking diptera and important vectors of various pathogens including protozoas from the order Kinetoplastida. In my bachelor work I am dealing with distribution and development of monoxenous and heteroxenous trypanosomatids in sandflies, with special attention to non-leishmania trypanosomatids.
Phlebotomine saliva: the perspecives of application of some of their recombinantly produced proteins
Rožníčková, Lucie ; Zídková, Lenka (advisor) ; Štáfková, Jitka (referee)
Sandflies (Diptera: Phlebotominae) are the vectors of one of the most significant tropical diseases, leishmaniosis. Their saliva contains substances with antihemostatic, vasodilatory and immunomodulatory effect. These substances are of great significance in transmission of leishmaniosis, because of their ability to influence the host immune response. Naive host saliva modulate the immune system to such an extent, that it reduces an infective dose and increases the pathogenicity of leishmaniosis. On the other hand, a repeatedly exposed host generates a cellular and antibody immune response, which is able to make the initial development of infection impossible. Repeated exposure of individual sandflies lead to formation of antibodies against components of saliva, which may play a role as a rate exposure indicator and thus the risk of leashmaniosis infection. These proteins and glycoproteins can be produced by recombinant DNA technology. Recombinant vaccine could help in the fight against leishmaniasis. Keywords: saliva, sand fly, leishmania, recombinant proteins.
Kinetoplastida of birds and bloodsucking Diptera: molecular characterization and life cycles
Zídková, Lenka ; Svobodová, Milena (advisor) ; Fiala, Ivan (referee) ; Modrý, David (referee)
We studied biodiversity of avian trypanosomes. We used newly obtained isolates of trypanosomatids from ornitophilic bloodsucking diptera (81) and from avian hosts (145). To investigate relationships among isolates we employed RAPD method and sequence analyses of SSU rRNA, eventually gGAPDH, 5S RNA or SL RNA. Additionally we used morphological characters; cell lengths and kinetoplast widths. All isolates obtained from biting midges belonged to monogenetic kinetoplastids. We described new species and a new genus Sergeia podlipaevi isolated from Culicoides (Oecacta) festivipennis a C. (O.) truncorum. We successfully infected with this species laboratory bred biting midge of different subgenus Culicoides (Monoculicoides) nubeculosus. We described isolate from Culicoides truncorum as new species Herpetomonas trimorpha. Its sister species was H. ztiplika described previously also from biting midges. We performed the most extensive study of biodiversity of avian trypanosomes based on comparison of wide spectrum of isolates obtained from different hosts. We confirmed that avian trypanosomes are polyphyletic and form three independent lineages within the genus Trypanosoma. We identified at least 11 species of avian trypanosomes. We found that ornitophilic mosquitoes belong among important vectors of avian...

See also: similar author names
4 ZÍDKOVÁ, Lucie
1 Zídková, Lea
1 Židková, Leona
4 Žídková, Lucie
Interested in being notified about new results for this query?
Subscribe to the RSS feed.