National Repository of Grey Literature 14 records found  1 - 10next  jump to record: Search took 0.00 seconds. 
A Preliminary Study of Modification of 7-Deazaadenine with a Complex of Osmium Tetroxide with 2,2 '-Bipyridine
Vítová, Lada ; Havran, Luděk ; Fojta, Miroslav ; Šedo, O. ; Zdráhal, Z. ; Vespalec, Radim
Reactivity of a purine nucleic base analogue 7-deazaadenine (A*) in short oligodeoxynucleotide (ODN) towards an osmium tetroxide complex with 2,2'-bipyridine (Os, bipy) was investigated by means of electrochemistry, capillary electrophoresis and MALDI-TOF mass spectrometry. Electrochemical measurement, electrophoretic analyses and mass spectrometric analyses of reaction mixture proved that ODN with an A* residue yields an osmium adduct with properties similar to those exhibited by the well-known adduct of thymine.
Study on the Electrophoretic Behaviour of Short Oligodeoxyribonucleotides in Fused Silica Capillaries. A Preliminary Communication
Vítová, Lada ; Fojta, Miroslav ; Vespalec, Radim
Effects of background electrolyte pH, composition and concentration on the electrophoretic transport of short oligodeoxyribonucleotides (ODNs) in fused silica capillaries have been investigated in the presented study. Mobilities and separation efficiencies of ODNs peaks varied with all three background electrolyte characteristics. Just changes in the background electrolyte characteristics and acid-base properties of ODNs are not sufficient for a systematic explanation of the observed variations. Despite the indicated complexity of ODNs electrophoretic behaviour, we show that very high separation efficiencies of ODNs peaks can be achieved in background electrolytes of physiological pH.
Electrochemical Investigation of Different Boron Cluster Compounds in Water-based Electrolytes
Fojt, Lukáš ; Vespalec, Radim ; Fojta, Miroslav ; Grüner, Bohumír
Boron cluster compounds (BCC) belongs to one or the proud sing compounds which ace used for medical treatment Their use for biochemical purposes is connected with use of water-based environments. Up to now, the BCC were predominantly characterized in non-aqueous milieus Therefore, we decided to undertake electrochemical research of BCC in water-based electrolytes. We have investigated several boron clusters with structures containing different number of vertexes (from 3 to 18), The structures were modified by endo- and exo- skeletal substituents. All used BCC were electrochemically active in broad potential region. which implies their possible use as biomarkers with tunable electrochemical response.
Capillary Electrophoresis in the Analysis of Chiral Species
Vespalec, Radim
An overview of methodology of chiral separations by using capillary electrophoresis in free solution is given.
Mobility in capillary electrophoresis
Vespalec, Radim
Basic terms in capillary electrophoresis are defined and discussed.
Liquid chromatography and capillary electrophoresis - partners or rivals?
Vespalec, Radim
Advantages, limitations and complementarity of both instrumental separation techniques were discussed.
pK determination of sparingly soluble weak bases
Vlčková, Markéta ; Barták, P. ; Vespalec, Radim
pK values of sanguinarine and chelerythrine have been determinedat conditions of planned interaction measurements and renasons for the pread of published pK data of these weak bases have been investigated.
Chiral separation of charged cluster boranes
Slavíček, V. ; Vespalec, Radim
Capillary zone electrophoresis is effective instrumental technique for chiral separations of charged cluster boranes if β-cyclodextrin serves as chiral selector.
Study of electrophoretic properties of sanguinarine and chelerythrine
Vlčková, Markéta ; Vespalec, Radim
PK values of alkaloids sanguinarine and chelerythrine were determined using capillary zone electrophoresis. UV-absorption spectra of both forms of these alkaloids, which are necessary for photometric detection, were therefore measured, too.
Chiral separations of charged boranes and carboranes by CZE
Slavíček, Viktor ; Vespalec, Radim
The applicability of capillary zone electrophoresis for achiral and chiral analysis of deltahedral boranes was proved. Twelve derivatives of different structural types were chirally separated with ß-cyclodextrin as a chiral selector.

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