National Repository of Grey Literature 7 records found  Search took 0.00 seconds. 
Resynthesis of oilseed rape from Brassica rapa and Brassica oleracea
Klíma, Miroslav ; Hilgert-Delgado, Alois ; Ulvrová, Tereza ; Bryxová, Pavla ; Vítámvás, Pavel ; Prášil, Ilja Tom ; Kosová, Klára
In order to extend the variability of the genofond, so-called resynthesis can be used, when new genotypes are created by crossing selected Brassica rapa and B. oleracea, by early isolation of immature embryos from young eggs and their in vitro fertilization (Hilgert-Delgado et al., 2015). It has been shown that resyntetized genotypes are distinct from rape materials grown at present (Sosnowska et al., 2010), so that they can serve as a source of new variability in breeding programs focusing on cytoplasmic and pollen sterility, resistance to pathogens and tolerance to stresses (Warwick et al., 2009). Representatives of the initial components are characterized by wide variability in terms of morphology and agronomic characteristics, and are a prospective source of heterosis and other traits (Wu et al. The use of resyntetized genotypes for the direct production of rapeseed varieties is not possible due to the poor quality of the seed of these materials. It is mainly the high content of glucosinolates and erucic acid, typical of most of the initial components (Jesske et al., 2013). However, the required properties can be transferred by crossing to conventional breeding materials (Hilgert-Delgado et al., 2017).
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Determination of the frost tolerance of rapeseed varieties in early stages of plant development
Prášil, Ilja Tom ; Klíma, Miroslav ; Musilová, Jana ; Kosová, Klára ; Vítámvás, Pavel
The methodology is focused on the determination of frost tolerance of varieties, breeding materials and gene sources of rapeseed in the early stages of plant development using a direct frost test. Rapeseed plants grow in pots located outdoors under natural conditions. The sowing of germinated rapeseed seeds is carried out during October so that until the end of November the plants reach the first leaf stage. The plants are exposed to a frost test in pots from December to February according to the described procedures.
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Dynamics of cold regulated proteins during cold acclimation in cereals
Vítámvás, Pavel ; Prášil, Ilja (advisor) ; Honys, David (referee) ; Smýkal, Petr (referee)
The aim of this dissertation was to study the mechanism of cold acclimation via the dynamics of cold regulated proteins (such as WCS120 or DHN5) in different frost- tolerant wheat and barley cultivars. Mass spectrometry analysis of a total sample of proteins, soluble upon boiling, showed qualitative differences between cold-acclimated (e.g., 7 COR proteins) and non-acclimated (e.g., only 3 COR proteins) samples of the winter wheat Mironovskaya 808. Furthermore, by 2-DE or W-blot analysis, there were found quantitative differences in the accumulation of WCS120 proteins between cultivars, grown under different time, photoperiod, and/or temperature conditions. The higher levels of WCS120 proteins are associated with higher frost tolerance of cultivars, grown under constant and low temperature. However, the dynamics of WCS120 proteins during long-term cold-acclimation, with periods of de-acclimation and re- acclimation, demonstrated that plants with the same level of frost tolerance could be distinguished by the level of accumulation of the WCS120 proteins. These results indicated that developmental genes influence the ability to re-accumulate WCS120 proteins by the partial vernalization of plants, while the ability to induce high frost tolerance was only influenced by the saturation of vernalization....
The physiological and proteomic characterisation of winter oilseed rape upon abiotic stress
Urban, Milan ; Vítámvás, Pavel (advisor) ; Hnilička, František (referee) ; Valentová, Olga (referee)
- Ph.D. thesis - Milan Urban, 2017 In some years, the agricultural production of oilseed rape, an important crop in the Czech Republic, is - besides biotic stress - facing the problem of damage caused by frost or drought. Together with special attention paid to proteins revealing responses between crop genotypes with differential abiotic stress tolerance levels we reviewed possible applications of proteomic results in crop breeding programs aimed at an improvement of crop stress tolerance (paper 1). For first original result, cold temperature was imposed upon non-vernalized plants in the stage of leaf rosette. The article (paper 2) shows a significant correlation between frost tolerance (FT), dehydrin (DHN) accumulation, and photosynthetic acclimation in five cultivars (cvs). Newly, the specific DHN D97 was shown to accumulate and other DHNs were shown to have qualitative differences in accumulation. These results imply that proper FT assessment is based on rapid photosynthetic acclimation together with higher accumulation of protective compounds. Drought stress (paper 3) was imposed in the water- demanding stem prolongation phase before flowering, because late-spring drought before and during flowering decreases the yield and seed quality significantly. This paper newly describes two water-uptake...
Transformation of potato with protease inhibitor gene SPI-2
Říhová, Barbora ; Fischer, Lukáš (advisor) ; Vítámvás, Pavel (referee)
The subject of my thesis was to genetically modify a potato for increased resistance against its pathogens and pests. In developing a resistant plant, it is quite common to use the same type of molecules that plants use themselves in their defense reactions. In this work I used the gene SPI-2 originating from a honeycomb moth (Galleria mellonella). The protein SPI-2 is a member of serine protease inhibitors. Since the previous attempts of the team to detect the protein in transformed plants haven't been successful, the basic form of the gene was modified by adding a Kozak sequence near the start codon, which should have increased the translation initiation and hence increase the level of the protein. Two constructs were prepared for the transformation: SPI-2-T a SPI-2-Y. They differ by one amino acid, which slightly changes their inhibitory activity. First, the construct SPI-2-T was used for a transient transformation of tobacco Nicotiana benthamiana by agroinfiltration of its leaves. Then both constructs were used for a stable transformation of Solanum tuberosum cv. Desireé. The detection of the protein has not been successful, although the inserted gene was transcribed and his sequence was verified by sequencing. It is therefore most likely that the protein has a low stability in the cytoplasm....

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