National Repository of Grey Literature 14 records found  1 - 10next  jump to record: Search took 0.01 seconds. 
Cell-penetrating peptides - pitfall of endosomal entrapment and its overcoming
Kadlecová, Julie ; Váňová, Jana (advisor) ; Holá, Dana (referee)
The aim of the thesis is to introduce cell penetrating peptides as a potential way of transporting cargo into cells. Their ability to penetrate cell membranes with attached cargo is demonstrated. The structure and sequences of these peptides, on which their unique property to penetrate through the cell membranes is built, are described with respect to the different modes of transport. The difficulty of internalizing these CPPs via endocytosis is the frequent trapping of peptide with attached cargo in the endosome. If this occurs, the endosome content is degraded and the CPP with attached cargo is not released into the cytosol or nucleus, their target site. This work focuses on creative options, how to make release from endosome easier for CPPs with attached cargo. The introduction of such modified CPPs would accelerate their use in clinical medicine to enable the treatment of diseases such as cancer or antibiotic- resistant diseases.
Directed evolution of mouse polyomavirus
Váňová, Jana ; Španielová, Hana (advisor) ; Mašek, Tomáš (referee)
The method of directed evolution represents a new approach to generate proteins with new or altered properties. The principle of directed evolution is random mutagenesis of the coding sequence for a protein of our interest followed by selection of generated mutants for the desired property. The aim of this pilot study was to investigate the possibility of utilization of directed evolution for alteration of mouse polyomavirus original tropism and virus retargeting to a model prostate cancer cell line. To generate randomly mutated gene encoding the major capsid protein of mouse polyomavirus, which is responsible for the interaction of the virus with cellular receptor for viral cell entry, error-prone PCR and DNA shuffling methods were used. Production of viruses composed of mutant major capsid protein was ensured by Cre/loxP site-specific recombination. The thesis also dealt with the design and characterization of the system for viral mutant selection. It was found that the prostate cancer cell lines markedly vary in their ability to bind and internalize particles derived from mouse polyomavirus. This knowledge can be used for the preparation of virus-like particles for prostate cancer diagnostics in the future. The study demonstrated that the method of directed evolution can be used for production...
Publishing Strategies in the Field of Children's Literature
Váňová, Jana ; Mocná, Dagmar (advisor) ; Hník, Ondřej (referee)
This thesis focuses on the exploration and interpretation of publishing strategies used by local Czech publishing companies concerning children's literature. This thesis offers techniques and methods used by publishers in order to increase sales. These strategies are particularly applied in a book promotion and their web site's presentation. A variety of different publishing companies were chosen for the purpose of research and comparison. The publishing company Baobab presents an alternative publisher and is considered an elite due to many book awards it has received. It focuses mainly on the incorporation of literary and visual aspects of the book creating a whole artwork. Publishing company Albatros with a reputation of a first-rate publisher during the pre-revolution era. Its children's production is still high-rated despite its economic transformation in the last twenty years. And finally a purely commercial publishing house Knižní klub which does not specialize in children's literature but tries to offer the widest range of books. Even though every publisher follows the same aim to distribute a book to a reader, each finds its own way to achieve the goal, which is very much evident in their strategies. The Baobab tries to publish an art book, which enriches young readers contrary to Knižní...
Retargeting of viral particles for directed cargo delivery into cells
Váňová, Jana ; Španielová, Hana (advisor) ; Kuthan, Martin (referee)
Due to their structure and ability to enter cells where they release their content, viral particles represent an attractive tool to deliver cargo to a cell. For therapeutic usage of viruses it is necessary to ensure the specific and highly efficient entry to target cells. This thesis offers an overview of methods used for virus retargeting with the intention to evaluate the success of retargeting in terms of specificity and efficiency of designed viral particles when entering a cell. On the basis of published data and considering the mechanisms of viral infection, the thesis demonstrates the difficulty to prevent the nonspecific viral particles from entering the cell and concludes that reaching total specificity is apparently impossible. Despite these small limitations, viral nanoparticles are a revolutionary therapeutic tool for delivering cargo to the cell and it is necessary to exploit their potential.
Closing of a Nursery School Managed by a Community. Crisis Management
Váňová, Jana ; Karabec, Stanislav (advisor) ; Báča, Milan (referee)
This closing work deals with the process of liquidation of nursery schools founded by a parish. It summarises a procedure that was used for the liquidation of nursery schools in Česká Lípa. It evaluates the process on the basis of a questionnaire filled out by five schoolmistresses from cancelled nursery schools. This closing work aims to create an optimal procedure based on these findings. It focuses on the duties of schoolmistresses from the cancelled nursery schools during the process of liquidation, who carried the main responsibilities for the successful liquidation of a nursery school. The aim is to identify the most important tasks. It draws from universal legal conditions of law and forms a Contributory organization with the emphasise on nursery schools. It is based on the analysis of the schoolmistress's duties during the liquidation of a nursery school. It attempts to describe the view of a present schoolmistresses on critical proceedings with the help of the questionnaires. It gives a comprehensive view of the liquidation process of a nursery school with the emphasise on a schoolmistresses activities in the area of critical management. It also talks about management diversification and about leading people. The results of the work may be useful not only for other directors and schoolmistresses of...
Biological activity of plant metabolites IX. Antimicrobial activity of some secondary metabolites
Váňová, Jana ; Opletal, Lubomír (advisor) ; Karlíčková, Jana (referee)
Váňová, J.: Biological Activity of Plant Metabolites IX. Antimicrobial Activity of Some Secondary Metabolites. Diploma Thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové 2008, 62 pp. The antibacterial activity of 4 selected plant extracts was evaluated against E. coli O157:H7 and Staphylococcus aureus. Extracts were prepared by extraction in 80% ethanol and subsequent percolation. Two methods were used for determination of minimal inhibitory concentration (MIC). In the first case they were not reached the values of MIC expected for E .coli on the basis of the other literature studies. All samples within the concentration range of 5 - 0,005 ppm which were prepared in two series were negative even after 48 hours exposition. In the second case it was reached better results for E. coli and S. aureus. Two series of serial twofold dilutions within the concentration range of 25 %-0.02 % were prepared. The lowest values of MIC were exhibited by Thymus vulgaris extract, especially against Gram-positive indicator strain. Nevertheless the values of MIC remain very high (3.13 % for E. coli, 0.2 % for S. aureus). For determination of minimal bactericidal concentration (MBC) in E. coli O157:H7 and E. coli O55 seven...
The biological activity of compounds contained in plants XVIII. Antimicrobial activity of some secondary metabolites 2.
Váňová, Jana ; Siatka, Tomáš (referee) ; Opletal, Lubomír (advisor)
Váňová, J.: Biological Activity of Plant Metabolites XVII. Antimicrobial Activity of Some Secondary Metabolites 2. Rigorous Thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové 2009, 106 pp. The antibacterial activity of 4 selected plant extracts was evaluated against E. coli O157:H7 and Staphylococcus aureus. Extracts were prepared by extraction in 80% ethanol and subsequent percolation. Two methods were used for determination of minimal inhibitory concentration (MIC). In the first case the values of MIC expected for E .coli on the basis of the other literature studies were not reached. All samples within the concentration range of 5 - 0,005 ppm which were prepared in two series were negative even after 48 hours exposition. In the second case better results were reached for E. coli and S. aureus. Two series of serial twofold dilutions within the concentration range of 25 %-0.02 % were prepared. The lowest values of MIC were exhibited by Thymus vulgaris extract, especially against Gram-positive indicator strain. Nevertheless the values of MIC remain very high (3.13 % for E. coli, 0.2 % for S. aureus). For determination of minimal bactericidal concentration (MBC) in E. coli O157:H7 and E. coli O55 seven essential...
International trade in pangolins (\kur{Manis} spp.)
VÁŇOVÁ, Jana
The aim of this work was to evaluate international trade with Manis. spp. according to the CITES database during the period 2002-2013. Manis spp. was classified in appendix II CITES in 1995. In 2000 there was an unsuccesfull attempt to reclassified Manis spp. to appendix I CITES. In this year the zero quota was set for Asian pangolins for commercial purpose. A rapid increase of an illegal trade was noticed after the setting of zero quota. The most frequent trade with pangolins was in these forms: 1) derivatives, 2) specimens, 3) scales, 4) live pangolins and 5) skins. Asian pangolins´ species were traded mostly in forms of derivatives, specimens, scales and skins in the period 2002-2013. M. javanica and M. pentadactyla with medium and large distribution area were quantitatively the most traded species, respectively. Main importers of Asian forms of pangolins were: USA (derivatives and specimens), China (scales in kg) and Indonesia (scales in pieces) and Mexico (skins). The African pangolin M. tricuspis was the third most frequent species in the international trade and was traded especially in form of live pangolins. M. tricuspis lives in one of the largest distribution area. Main importer of live pangolins was Italy. Breeding pangolins in captivity is due to the specific feeding requirements demanding. There are about 46 individuals of pangolins in Zoos all over the world, whereas in Europe there are only 2 pangolins in the Leipzig Zoo.

National Repository of Grey Literature : 14 records found   1 - 10next  jump to record:
See also: similar author names
14 Vaňová, Jana
2 Vaňová, Jaroslava
1 Vaňová, Jiřina
14 VÁŇOVÁ, Jana
2 VÁŇOVÁ, Jaroslava
3 Váňová, Jarmila
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