National Repository of Grey Literature 5 records found  Search took 0.00 seconds. 
Development of inhibitors of rhomboid proteases as tools for the study of their biological functions
Tichá, Anežka ; Stříšovský, Kvido (advisor) ; Šedo, Aleksi (referee) ; Konvalinka, Jan (referee)
Rhomboids are intramembrane serine proteases that belong to the evolutionarily widespread rhomboid superfamily. Rhomboids developed a slightly different catalytic mechanism compared to classical serine proteases; they utilise a catalytic dyad (Ser/His) instead of the common triad (Ser/His/Asp), and the rhomboid active site is buried in the membrane. This, coupled with their hydrophobicity, makes them quite difficult to study. Therefore, even though they are known to be involved in several important biological processes it is still not clear how exactly most of them are involved in the regulation of or in the pathologies of diseases related to these processes (such as malaria, Parkinson's disease or cancer). Our understanding is hindered by the lack of tools for their characterisation both in vitro and in vivo. In my thesis I present new fluorogenic substrates based on the LacYTM2 sequence, which is hydrolysed by several different rhomboid proteases. Using Förster resonance energy transfer (FRET)-based methods, these substrates are suitable for continuous monitoring of rhomboid activity in vitro. Modifications in the P5-P1 residues can improve selectivity for a specific rhomboid, the choice of FRET pair of fluorophores that absorbes light of longer wavelengths makes them suitable for high throughput...
Spokojenost zaměstnanců ve vybraném podniku
TICHÁ, Anežka
The main topic of this study is the employee satisfaction survey. The aim is to evaluate all of the factors influencing work satisfaction and the overallsatisfaction of employees in a chosen company. I focused on a local branch of Kaufland v. o. s., Hradec Králové. For this survey I chose the method of anonymous questioning. I created a questionnaire with 19 questions, which I distributed on paper form at a branch. After filling it in, I counted up answers to each of questions, and this I utilized into the tables. After this I created the graphs for each of the factors. It is possible to read, from these graphs, that the employees of the Kaufland branch are mostly satisfied. But factors such as the reward system, the signs of fluctuation or the chance of self-education shoved lover degree of satisfaction. The least satisfaction is shown in the system of empoyee benefits. Following these results it was suggested some arrangement, which should lead to higher satisfaction in individual factors. The results of this study were handed over to a company that can make changes and improve the satisfaction of employees.
The in vitro study of the new lipidized analogs of prolactin-releasing peptide
Šatrová, Lucie ; Maletínská, Lenka (advisor) ; Tichá, Anežka (referee)
Bakalářská práce se zabývá neuropeptidy ovlivňujícími příjem potravy anorexigenním účinkem (snižujícím příjem potravy), které jsou potenciálními Mezi tyto neuropeptidy se řadí peptid uvolňující prolaktin (PrRP), který se váže s itou ke svému receptoru GPR10, a také k afinitou jen o řád nižší než laboratoři RNDr. Lenky Maletínské, CSc. byly navrženy a syntetizovány nové lipidované analogy PrRP, které mají podobnou afini receptorům GPR10 i NPFF2 přirozený PrRP a snižují příjem potravy po periferním podání. Na základě skutečnosti, že GPR10 má vysoko jedním receptorem označovaným jako Y1 a receptor Y1 je homologický s dalšími íněných peptidů Y2 a Y5, byla této bakalářské práci stanovena afinita přirozeného a jeho dvou palmitovaných analogů k receptorům Na buňkách U2OS s transfekovanými pomocí saturačních vazeb . Ty sloužily k výpočtu kompetitivních vazebných experimentech, kde přirozený a jeho dva palmitoylované měly velmi nízkou buňkám U2OS s transfekovanými receptory Y1 nebo Y2. Na druhé straně přirozený PrRP vázal buňkám transfekovaným receptorem řádu 10 a jeho palmitoylované analogy s až stokrát nižší Součástí bakalářské práce byla i optimaliz a přítomnosti inhibitorů proteáz stanovení K buněčn sfekovaným NPFF2 Stanovená K řádu 10 mol/l podobná hodnotám pro přirozené ligandy receptorů Zkoumání vazby...
New analogues of prolactin-releasing peptide with prolonged effect on food intake
Tichá, Anežka ; Ryšlavá, Helena (advisor) ; Konvalinka, Jan (referee)
Prolactin-releasing peptide (PrRP) is a member of the family of RF-amide peptides. These peptides have typical C-terminal sequence -Arg-Phe-NH2 and similar biological effects. PrRP was discovered as an endogenous ligand of an orphan receptor GPR10 while searching for a factor responsible for a prolactin secretion. This effect was not later confirmed and nowadays, PrRP is mainly considered as an anorexigenic peptide. This is supported by a fact that PrRP and GPR10 deficient mice suffer from hyperphagia and late-onset obesity. Besides GPR10, PrRP is bound to NPFF2 receptor whose endogenous ligand is neuropeptide FF (NPFF). In this study, the PrRP's analogues modified at the N-terminus with fatty acids of different lenghts were tested in vitro on binding and activation MAPK/ERK1/2 signalling pathway. In in vivo experiments on food intake, the central anorexigenic effects of lipidized PrRP-analogues were tested provided their crossing blood brain barrier. Binding studies showed that all analogues bound to rat pituitary RC-4B/C cells with high affinity, analogues containing fatty acid with Ki of one order of magnitude lower than native PrRP. High affinity was also confirmed for binding to cells overexpressing GPR10 receptor and cell membranes with overexpressed NPFF2 receptor. All tested analogues...
Effects of PrRP (prolactin-releasing peptide) and NPFF (neuropeptide FF) analogs in vitro and in vivo
Tichá, Anežka ; Ryšlavá, Helena (advisor) ; Železná, Blanka (referee)
Prolactin-releasing peptide (PrRP) and neuropeptide FF (NPFF) belong to the RF-amide family. These peptides have identical C-terminal amino acid sequence (R-F-NH2) and similar biological activities. PrRP was identified as an endogenous ligand of an orphan receptor GPR10 able to stimulate PRL-secretion in vitro and in vivo, but soon it was discoverd that this is not the primary function of this peptide. PrRP is thought to be an anorexigenic peptide as PrRP and GPR10 are found in several parts of the brain responsible for food intake regulation and because both GPR10 and PrRP deficient mice suffer from hyperphagia and late-onset obesity. In this study, relationship between PrRP and NPFF was studied using both in vitro binding and sell signaling and in vivo food intake and analgesia test in mice. In vitro experiments showed that PrRP bound to rat pituitary RC-4B/C cells containing GPR10 receptor with high affinity and NPFF, its stable analog 1DMe and its antagonist RF9 up to 10-5 M concentration did not bind to GPR10. NPFF, 1DMe and PrRP were bound to cell membranes with transfected NPFF2 receptor with high affinity, but RF9 with low affinity in a range of 10-7 M, in contrast to published literature. In vivo experiments with fasted mice confirmed that centrally injected PrRP and NPFF significantly...

See also: similar author names
2 TICHÁ, Anděla
4 TICHÁ, Anežka
1 TICHÁ, Anna
6 Tichá, Alena
2 Tichá, Alice
4 Tichá, Alžběta
2 Tichá, Andrea
2 Tichá, Anděla
1 Tichá, Aneta
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