National Repository of Grey Literature 394 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Determinants of the splice site selection in protein-coding and long non-coding RNAs
Krchňáková, Zuzana ; Staněk, David (advisor) ; Svoboda, Petr (referee) ; Blažek, Dalibor (referee)
In my thesis, I focused on several underexplored areas of RNA splicing regulation. In the first part, I analyzed how chromatin and transcription regulatory elements change pre-mRNA splicing. In the second part, I studied why long non-coding RNAs (lncRNAs) are spliced less efficiently than protein-coding mRNAs. Finally, I was testing the importance of intron for the activating function of lncRNAs. It has been shown that chromatin and promoter identity modulate alternative splicing decisions. Here, I tested whether local chromatin and distant genomic elements that influence transcription can also modulate splicing. Using the chromatin modifying enzymes directly targeted to FOSL1 gene by TALE technology, I showed that changes in histone H3K9 methylation affect constitutive splicing. Furthermore, I provide evidence that deletion of transcription enhancer located several kilobases upstream of an alternative exons changes splicing pattern of the alternative exon. Many nascent lncRNAs undergo the same maturation steps as pre-mRNAs of protein- coding genes (PCGs), but they are often poorly spliced. To identify the underlying mechanisms for this phenomenon, we searched for putative splicing inhibitory sequences. Genome-wide analysis of intergenic lncRNAs (lincRNAs) revealed that, in general, they do not...
Detention of foreigners
Hametová, Andrea ; Kryska, David (advisor) ; Svoboda, Petr (referee)
Administrative detention of migrants Resumé The aim of this thesis was to critically evaluate the institute of administrative detention of migrants and decide whether it is and appropriate, proportionate and necessary measure or if it has become obsolete considering the development of recent years and whether it would be appropriate to replace it with more effective alternatives. In the first part of the thesis I have critically evaluated the legal framework regarding administrative detention of migrants. I analysed the impact of international law with the emphasis of human rights, laid out the rules as stated by fragmented European law directives and regulations. The most detailed analysis is of Czech legal framework, including mentions of its most problematic points both from past and present. Next I considered the nature of the institute as an administrative law instrument used to restrict someone on his personal liberty. Considering the insufficiency of mere legality as a criterion of legality and legitimacy of administrative detention, I evaluated the aims and purposes of the administrative detention of migrants. In the last part of the thesis I have raised some critical points against current legal framework and practice. I point out the abuse of administrative detention for other than legally stated...
Joint procedure to grant planning and building permission
Souralová, Natálie ; Svoboda, Petr (advisor) ; Staša, Josef (referee)
This thesis deals with joint procedure to grant planning and building permission. The thesis' aim is to summarize the legal development of the joint procedure to grant planning and building permission between the years 2007 and 2018 and to focus on its problematic aspects, which are relevant in legal regulations, and subsequently to describe the possibilities for their appropriate solution. The sub goal of this thesis includes the outline of possible changes in connection with an envisaged recodification of the Czech Public Construction Law, in which the joint procedure to grant planning and building permission plays a key role. This thesis is divided into four main chapters aiming to cover the joint procedure to grant planning and building permission. The first chapter covers an introduction to the joint procedure to grant planning and building permission and focus on the relationship between the Act No. 500/2004 Coll., Administrative Act, as amended and the Act No. 183/2006 Coll., on Town and Country Planning and Building Code (Building Act), as amended. This chapter further aims at defining the basic principles which are necessary for the joint procedure to grant planning and building permission. The purpose of the second chapter is to provide a general comprehensive overview of the legal...
Legal aspects of the current personal data protection developments
Tumpachová, Kateřina ; Pítrová, Lenka (advisor) ; Svoboda, Petr (referee)
This doctoral thesis is divided into ten chapters in which, together with the introduction and conclusion, it aims to describe and analyse the current development of legal regulation of personal data protection, especially from a substantive law perspective. The thesis analyses the existing legal regulations of the European Union, implemented in the legal frame of the Czech Republic compared to the new General Data Protection Regulation, which unifies the legal framework for the personal data protection in the whole European Union. The first chapter of the thesis describes in brief the development of the personal data protection legal framework, from the establishment of the right to personal data protection in human rights declarations to the gaining its independence from the right to privacy. Furthermore, the relevant OECD Guidelines and Convention 108, which plays a crucial role in the field of personal data protection, are described. The development of the legal regulation of personal data protection in the European Union, together with the development of legislation in the Czech Republic, which also meant the necessary implementation of EU regulations into the Czech legal order with the accession of the Czech Republic to the European Union, are described. The second chapter describes in detail...
Long Non-Coding RNAs in Oocyte-to-Embryo Transition
Ganesh, Sravya ; Svoboda, Petr (advisor) ; Vanáčová, Štěpánka (referee) ; Shkumatava, Alena (referee)
(English) Oocyte-to-embryo transition (OET) is one of the most complex developmental events, during which a differentiated oocyte gives rise to a totipotent zygote. During OET a transcriptionally silent oocyte undergoes massive reprogramming of gene expression, which transforms it into a transcriptionally active zygote. Although numerous studies have contributed to understanding the mechanism of OET, many genes involved in OET are yet to be identified. A whole new level of possible regulation of OET came with the discovery of long non-coding RNAs (lncRNA). LncRNAs are pol II transcripts longer than 200 nucleotides, that are typically spliced and polyadenylated but do not encode proteins. While lncRNAs have been studied in many model systems including embryonic stem cells, their expression in oocytes and early embryos and contribution to OET were largely unexplored at the beginning of this project. In my PhD project, I aimed to identify, annotate, and analyze lncRNAs expressed during OET. First, using RNA-Seq, 1600 highly reliable lncRNAs were identified and annotated in mouse oocytes and early embryos. Majority of lncRNAs were novel with expression exclusively at OET stages. A significant fraction of these lncRNAs was found associated with LTR retrotransposons, contributing to their novelty and...
Securing of public order in the Czech Republic and the State of Israel
Pavlátová, Michaela ; Millerová, Ivana (advisor) ; Svoboda, Petr (referee)
Topic of this thesis is securing public order in the State of Israel and comparing it with securing public order in the Czech Republic. In view of the current security situation in Europe and its development, the Czech Republic is also currently facing new challenges in the area of securing public order. The State of Israel is a country that has been facing threats of destruction from its enemies from the day of Israel's creation. Ensuring security and the sole existence of the State has become a major priority for Israel, and thus studying Israel's ways to secure public order is, in my view, useful and in certain aspects also inspiring. This thesis presents a detailed view of Israel's methods of securing public order. It also reflects the social and historical context which is an integral part of the Israeli public order legislation and which is essential for correct understanding of the Israeli approach to provide security to its inhabitants. I compare the most important aspects of securing public order in the State of Israel with the ways of securing public order in the Czech Republic. This thesis is divided into six chapters. The first two chapters are dealing with the Czech concept of the public order and the Czech methods of securing public order. The third chapter analyzes the term "public...
Territorial decision-making processes
Šobora, Jan ; Svoboda, Petr (advisor) ; Millerová, Ivana (referee)
Thesis title: Territorial Decision-Making-Processes In this thesis, the author deals primarily with the planning permission proceedings, including the analysis of partial changes in the legislation introduced by the conceptual amendment of Act No. 183/2006 Coll., On Spatial Planning and the Building Code (Building Act), which came into effect on January 1, 2018. Author of this thesis draws in his writing primarily from the available commentary literature, from various judicial decisions of the Czech courts, especially of the Supreme Administrative Court of the Czech Republic and, last but not least, from a number of expert articles. The thesis is internally divided into the introduction, three separate chapters, subchapters and the conclusion. In the introductory chapter, the author defines selected basic terms relevant to planning permission proceedings in order to give the reader the opportunity to better understand the subject matter. The author further compares the differences between conceptual and implementation tools of spatial planning, describes the ways in which buildings are placed in the territory, as well as the different types of planning permits. In the second, core chapter of the thesis, the author offers a detailed analysis of the process leading to the issuance of a planning...
The comparison of legal regulations of the compulsory vaccination in the Czech Republic and the French Republic
Ševčík, David ; Svoboda, Petr (advisor) ; Kryska, David (referee)
v anglickém jazyce (Abstract) The aim of this diploma thesis is to analyze and compare the system of compulsory vaccination in the Czech Republic and in the French Republic. The first part of this diploma thesis is dedicated to international law context of compulsory vaccination. Relevant international organizations in which is the Czech Republic and also French Republic taking part is described. Then the focus of interest is also on all relevant binding sources of international law, or more precisely on promulgated international treaties, ratified and by which the Czech Republic and the French Republic is bound with the rule that if a treaty provides something other than national law, the treaty shall apply. The second part of this diploma thesis is dedicated to the connection between compulsory vaccination and constitutional law of the Czech Republic and French Republic, or rather how could be compulsory vaccination justified by the constitutional law of both countries. The case law coming from supervising constitutional institutions related to compulsory vaccination is also examined. In the third part, the legal regulation of key aspects of compulsory vaccination is examined. The focus of interest is on the amount of regulation made by statute and by secondary legislation. The aim of this part...
Protection against delays in administrative proceedings and in proceedings before administrative court
Koudele, Lukáš ; Kryska, David (advisor) ; Svoboda, Petr (referee)
Protection against delays in administrative proceedings and in proceedings before administrative courts Abstract The subject of this work are delays in administrative proceedings (where its legislation marks them as inaction) and before administrative courts. A delay is a state in which a certain act in the proceedings for some reason is not undertaken at the time within which it should be done. Reasons for delays may vary. They may be objective, which may consist of a number of cases that a person decides, whether it is due to insufficient staffing of the authorities in charge of the proceedings, or because of the deficiencies in the organization of work with that authorities, or may consist in the necessity of following the sequence of a process involving the necessity to perform an act that precedes the given operation. Reasons for delays can also be subjective, due to the lack of speed of work, either due to laziness or incompetence. The Charter of Fundamental Rights and Freedoms speaks of the duty to discuss the matter without unnecessary delays. This obligation applies both to administrative proceedings and to proceedings at the administrative courts. Since this is a law regulation, which applies to constitutional order, other legal norms can not contain a rule that would be inconsistent with the...
Creating a biosensor for miRNA effector complex formation using CRISPR nucleases
Petržílek, Jan ; Svoboda, Petr (advisor) ; Petr, Jaroslav (referee)
miRNAs are small regulatory RNAs, which function as post-transcriptional mRNA regulators. They direct ribonucleoprotein complexes to cognate mRNA to repress them by translational inhibition and degradation. miRNAs regulate thousands of mRNAs in mammals and have been recognized as regulatory factors in most cellular and developmental processes. Dysregulation of the miRNA pathway can lead to severe defects and diseases. Interestingly, a unique situation exists in mouse oocytes, where all the miRNA pathway components are present, yet the pathway is dispensable and nonfunctional, the molecular foundation of this phenomenon and its significance still remain unclear. In spite of the pronounced effects of the miRNA pathway in gene regulation in somatic cells, study strategies of the pathway bare limitations. Current methods for studying the activity of the miRNA pathway employ corelative studies (such as NGS) or reporter assays, which have relatively low throughput and are prone to artifacts. Here, I present design and development of a new strategy for directly monitor global miRNA pathway activity and integrity in near physiological conditions in living cells, which could also be employed in vivo for studies of mouse oocytes. The strategy is based on fluorescently tagged endogenous proteins of the...

National Repository of Grey Literature : 394 records found   1 - 10nextend  jump to record:
See also: similar author names
36 SVOBODA, Pavel
73 SVOBODA, Petr
1 Svoboda, P.
1 Svoboda, Patrik
36 Svoboda, Pavel
4 Svoboda, Peter
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