National Repository of Grey Literature 88 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Development of new types of biocompatible hemodialysis membranes for separation of biomolecules
Kohlová, Michaela ; Solich, Petr (advisor) ; Kujovská Krčmová, Lenka (referee) ; Pena, Angelina (referee)
Charles University, Faculty of Pharmacy in Hradec Králové / University of Porto, Faculty of Pharmacy Department of Analytical Chemistry / Department of Chemical Sciences Candidate: Mgr. Michaela Kohlová Supervisor: prof. RNDr. Petr Solich, CSc. Consultants: prof. Maria de Conceição Branco Montenegro assoc. prof. Alice Santos-Silva Title of dissertation thesis: Development of new types of biocompatible haemodialysis membranes for separation of biomolecules This dissertation thesis was focused on the development, preparation, and optimization of flat sheet polysulfone membranes, which would meet the characteristics required for membranes used for haemodialysis treatment. The thin porous membranes were prepared by spin coating method, followed by phase inversion via immersion precipitation. The composition, as well as the preparation process of the membranes, were optimized, to obtain a membrane with good mechanical performance in flow conditions, adequate selective separation ability, and biocompatibility. The prepared membranes with the appropriate characteristics were modified with bioactive compounds to minimize oxidative stress and/or inflammation, which are common complications in haemodialysis treated patients. Three different modification approaches were used to prepare the bioactive...
Development and optimization of chromatographic methods suitable for clinical application
Jenčo, Jaroslav ; Solich, Petr (advisor) ; Hroboňová, Katarína (referee) ; Šatínský, Dalibor (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Analytical Chemistry Candidate: Mgr. Jaroslav Jenčo Supervisor: prof. RNDr. Petr Solich, CSc. Consultant: doc. RNDr. Lenka Kujovská Krčmová, Ph.D. Title of dissertation thesis: Development and optimization of chromatographic methods suitable for clinical application The presented dissertation thesis is divided into several parts, copying the workplaces, which I gradually worked on. The unifying element is the development and optimization of methods for the preparation of samples of biologically active substances and their subsequent analysis using separation methods. The theoretical part characterizes the target substances, from a biochemical, physicochemical, and analytical point of view. It describes the basic characteristics of extraction procedures and presents an overview of the methods used to determine the substances described in the thesis. In the first, main experimental part, of the dissertation thesis the development, optimization, and validation of an analytical method for chromatographic analysis of vitamin B1, its biologically active forms and the active form of vitamin B6 in blood is described. It illustrates the selection of sample preparation techniques, the optimization of the target methodology...
Development of bioanalytical methods for the determination of diagnostic markers and drugs using chromatographic techniques
Javorská, Lenka ; Solich, Petr (advisor) ; Pohanka, Miroslav (referee) ; Šatínský, Dalibor (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Analytical Chemistry Candidate: Mgr. Lenka Javorská Supervisor: prof. RNDr. Petr Solich, CSc. Consultant: assoc. prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Title of dissertation thesis: Development of bioanalytical methods for the determination of diagnostic markers and drugs using chromatographic techniques The presented dissertation is focused on the development and validation of chromatographic method for the determination of glycopeptide antibiotic vancomycin, and L-arginine and its pathway related substances in biofluids. Furthermore, the potential of neopterin, kynurenine and tryptophan as biomarkers of immune response to surgical procedure and prediction of postoperative complications is investigated in connection with oncological diseases. In the theoretical part trends in instrumentation, stationary phases and extraction techniques are described. The issues of separation and retention of polar compounds and biological matrix pretreatment before analysis are also discussed there. Next part deals with selected analytes and their application to the clinical purpose for which the chromatographic methods are developed. It also provides an overview of published methods for their determination using liquid...
Development of new types of biocompatible hemodialysis membranes for separation of biomolecules
Kohlová, Michaela ; Solich, Petr (advisor) ; Kujovská Krčmová, Lenka (referee) ; Pena, Angelina (referee)
Charles University, Faculty of Pharmacy in Hradec Králové / University of Porto, Faculty of Pharmacy Department of Analytical Chemistry / Department of Chemical Sciences Candidate: Mgr. Michaela Kohlová Supervisor: prof. RNDr. Petr Solich, CSc. Consultants: prof. Maria de Conceição Branco Montenegro assoc. prof. Alice Santos-Silva Title of dissertation thesis: Development of new types of biocompatible haemodialysis membranes for separation of biomolecules This dissertation thesis was focused on the development, preparation, and optimization of flat sheet polysulfone membranes, which would meet the characteristics required for membranes used for haemodialysis treatment. The thin porous membranes were prepared by spin coating method, followed by phase inversion via immersion precipitation. The composition, as well as the preparation process of the membranes, were optimized, to obtain a membrane with good mechanical performance in flow conditions, adequate selective separation ability, and biocompatibility. The prepared membranes with the appropriate characteristics were modified with bioactive compounds to minimize oxidative stress and/or inflammation, which are common complications in haemodialysis treated patients. Three different modification approaches were used to prepare the bioactive...
Development of bioanalytical methods for the determination of diagnostic markers and drugs using chromatographic techniques
Javorská, Lenka ; Solich, Petr (advisor) ; Pohanka, Miroslav (referee) ; Šatínský, Dalibor (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Analytical Chemistry Candidate: Mgr. Lenka Javorská Supervisor: prof. RNDr. Petr Solich, CSc. Consultant: assoc. prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Title of dissertation thesis: Development of bioanalytical methods for the determination of diagnostic markers and drugs using chromatographic techniques The presented dissertation is focused on the development and validation of chromatographic method for the determination of glycopeptide antibiotic vancomycin, and L-arginine and its pathway related substances in biofluids. Furthermore, the potential of neopterin, kynurenine and tryptophan as biomarkers of immune response to surgical procedure and prediction of postoperative complications is investigated in connection with oncological diseases. In the theoretical part trends in instrumentation, stationary phases and extraction techniques are described. The issues of separation and retention of polar compounds and biological matrix pretreatment before analysis are also discussed there. Next part deals with selected analytes and their application to the clinical purpose for which the chromatographic methods are developed. It also provides an overview of published methods for their determination using liquid...
Stability Studies of Oral Liquid Preparations Using HPLC
Zahálka, Lukáš ; Solich, Petr (advisor) ; Kučera, Radim (referee) ; Muselík, Jan (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate Mgr. Lukáš Zahálka Supervisor Prof. RNDr. Petr Solich, CSc. Title of Doctoral Thesis Stability Studies of Oral Liquid Preparations Using HPLC According to the database of the Czech State Institute for Drug Control there are 59 thousand of registered drugs available in the Czech Republic, out of which 8 thousand drugs were marketed in July 2017; however, there are still some therapeutic needs that cannot be met by using of these commercially available drugs because of unsuitability of available dosage forms for being used, e.g., in pediatric patients. Thus, cooperation was established between the Faculty of Pharmacy in Hradec Králové (Department of Analytical Chemistry and Department of Pharmaceutical Technology) and the University Hospital in Motol (Prague) to address such therapeutic needs by developing of extemporaneous formulations of oral liquid preparations containing selected pharmaceutical active ingredients. Each project was composed of three major parts. First part was to develop several versions of drug formulations according to various requirements (e.g., sugar-free or preservative-free). This part was accomplished by the Department of Pharmaceutical Technology. The second (development...
Modern separation techniques for the analysis of biological material in clinical research
Kasalová, Eva ; Solich, Petr (advisor) ; Nobilis, Milan (referee) ; Friedecký, David (referee)
Charles University, Faculty of Pharmacy in Hradec Kralove Department of Analytical Chemistry Candidate: Mgr. Eva Kasalová Supervisor: Prof. RNDr. Petr Solich, CSc. Supervisor specialist: Assoc. Prof. RNDr. Dagmar Solichová, Ph.D. Title of Doctoral Thesis: Modern separation techniques for the analysis of biological material in clinical research This doctoral thesis deals with development and validation of a chromatographic determination of liposoluble vitamins and with optimization of biological materials preparation. Clinical monitoring of these vitamins, especially vitamin D in different biological materials is one of the most discuss theme in the last decade. In the theoretical part of this dissertation the target analytes - vitamins A, E and D with their biological activity are characterized - as well as the biological material used in the Research Laboratory, especially a detailed description of the composition of breast milk, its irreplaceable role in neonatal nutrition and description of breast milk processing in Milk Bank. This part is followed by a comprehensive chapter dealing with the treatment of the biological matrix before analysis. The most commonly used sample pretreatment techniques such as extraction methods are described here, with a great description of new trends in these...
Modern separation techniques for the analysis of biological material in clinical research
Kasalová, Eva ; Solich, Petr (advisor) ; Nobilis, Milan (referee) ; Friedecký, David (referee)
Charles University, Faculty of Pharmacy in Hradec Kralove Department of Analytical Chemistry Candidate: Mgr. Eva Kasalová Supervisor: Prof. RNDr. Petr Solich, CSc. Supervisor specialist: Assoc. Prof. RNDr. Dagmar Solichová, Ph.D. Title of Doctoral Thesis: Modern separation techniques for the analysis of biological material in clinical research This doctoral thesis deals with development and validation of a chromatographic determination of liposoluble vitamins and with optimization of biological materials preparation. Clinical monitoring of these vitamins, especially vitamin D in different biological materials is one of the most discuss theme in the last decade. In the theoretical part of this dissertation the target analytes - vitamins A, E and D with their biological activity are characterized - as well as the biological material used in the Research Laboratory, especially a detailed description of the composition of breast milk, its irreplaceable role in neonatal nutrition and description of breast milk processing in Milk Bank. This part is followed by a comprehensive chapter dealing with the treatment of the biological matrix before analysis. The most commonly used sample pretreatment techniques such as extraction methods are described here, with a great description of new trends in these...
Optimization of procedure for isolation of phosphorylated peptides from a peptide mixture by metal oxide affinity chromatography for mass spectrometry analysis
Popovská, Lenka ; Solich, Petr (advisor) ; Zemanová, Lucie (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Lenka Popovská Supervisor: Prof. RNDr. Petr Solich, CSc. Consultant Supervisor: Mgr. Barbora Šalovská, Ph.D. Title of the rigorous thesis: Optimization of procedure for isolation of phosphorylated peptides from a peptide mixture by metal oxide affinity chromatography for mass spectrometry analysis. Protein phosphorylation is the key regulatory mechanism in cellular signalling. It is involved in most cellular processes that are regulated by a complex interplay of protein kinases and protein phosphatases and include proliferation, differentiation, and apoptosis. Defective or altered signalling pathways can lead to various diseases, and therefore it is very important to study protein phosphorylation in detail and try to understand it as much as possible. Technological development of mass spectrometry (MS) in recent years has enabled a significant increase in the number of phosphorylation sites that may be identified in a sample. Increasing scan speed together with commercial availability of high resolution MS have improved quantity and quality of the acquired data, which can be obtained in a relatively short time. However, even high-end devices would not allow to comprehensively identify the...
On-line SPE HPLC method optimization for determination of patulin mycotoxin in apple drinks
Holznerová, Anežka ; Šatínský, Dalibor (advisor) ; Solich, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Anežka Holznerová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of Diploma Thesis: On-line SPE HPLC method optimization for determination of patulin mycotoxin in apple drinks The issue of food contamination with mycotoxins is a serious problem worldwide. These substances are highly toxic to humans and chronic effects on the human organism in very low doses may cause long-term medical complications. In our work we focused on one of the most common dietary mycotoxins - patulin, which is found in apples and related products (especially apple juices). High performance liquid chromatography (HPLC) coupled with on- line solid phase extraction (SPE) using a column switching technique for sample treatment was developed for determination of mycotoxin patulin in apple drinks and juices. A volume of 250 µl of juice sample was injected directly into the on-line SPE-HPLC system. After injection of the sample the extraction of patulin from juice matrix was carried out on SPE precolumn. SPE precolumn 25 x 3 mm was filled with Supel MIP Patulin sorbent, which is a specific "molecularly imprinted polymer" (MIP) designated for the selective extraction of patulin from an apple matrix. As the...

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