National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Resynthesis of oilseed rape from Brassica rapa and Brassica oleracea
Klíma, Miroslav ; Hilgert-Delgado, Alois ; Ulvrová, Tereza ; Bryxová, Pavla ; Vítámvás, Pavel ; Prášil, Ilja Tom ; Kosová, Klára
In order to extend the variability of the genofond, so-called resynthesis can be used, when new genotypes are created by crossing selected Brassica rapa and B. oleracea, by early isolation of immature embryos from young eggs and their in vitro fertilization (Hilgert-Delgado et al., 2015). It has been shown that resyntetized genotypes are distinct from rape materials grown at present (Sosnowska et al., 2010), so that they can serve as a source of new variability in breeding programs focusing on cytoplasmic and pollen sterility, resistance to pathogens and tolerance to stresses (Warwick et al., 2009). Representatives of the initial components are characterized by wide variability in terms of morphology and agronomic characteristics, and are a prospective source of heterosis and other traits (Wu et al. The use of resyntetized genotypes for the direct production of rapeseed varieties is not possible due to the poor quality of the seed of these materials. It is mainly the high content of glucosinolates and erucic acid, typical of most of the initial components (Jesske et al., 2013). However, the required properties can be transferred by crossing to conventional breeding materials (Hilgert-Delgado et al., 2017).
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Determination of the frost tolerance of rapeseed varieties in early stages of plant development
Prášil, Ilja Tom ; Klíma, Miroslav ; Musilová, Jana ; Kosová, Klára ; Vítámvás, Pavel
The methodology is focused on the determination of frost tolerance of varieties, breeding materials and gene sources of rapeseed in the early stages of plant development using a direct frost test. Rapeseed plants grow in pots located outdoors under natural conditions. The sowing of germinated rapeseed seeds is carried out during October so that until the end of November the plants reach the first leaf stage. The plants are exposed to a frost test in pots from December to February according to the described procedures.
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1 Prášil, Ilja
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