National Repository of Grey Literature 70 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
The in vitro effects of selected fenolic substances on isolated rat vessels
Rajtmajerová, Iveta ; Pourová, Jana (advisor) ; Hrdina, Radomír (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Iveta Rajtmajerová Supervisor: PharmDr. Jana Pourová, Ph.D. Title of diploma thesis: The in vitro effects of selected fenolic substances on isolated rat vessels Silymarin flavonolignans are endowed with various aspects. Recently their hepatoprotective effect has been discussed widely as well as their positive influence on the cardiovascular system. This diploma thesis is concerned with the vasodilatory effects of silybin-A, silybin-B, silybin-A + B and isosilybin-A on rat aorta. The aim of this study was to test the vasodilatory effect of selected substances. The dependence of aortic relaxation on the increasing concentration of tested substances was measured. Isolated Wistar breed rat aorta was used. DRC curves were created and EC50 values were assessed from the obtained values of vessel tension. The results were evaluated subsequently. The vasodilator potential of silybin-A and isosilybin-A is comparable; hence the position of the substituents may be irrelevant in having any impact on relaxation of the vessels. Different vasodilatory effects of silybin-A and silybin-B have been observed thus, the relaxation could be stereoselective. The testing of the equimolar mixture of silybin-A and...
Administration of cytostatic drugs and extravasation
Rohová, Marie ; Vopršalová, Marie (advisor) ; Pourová, Jana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové, Heyrovského 1203, Hradec Králové Department of pharmacology and toxicology Candidate: Mgr. Marie Rohová Consultant: PharmDr. Marie Vopršalová, CSc. Title of Thesis: Administration of cytostatic drugs and extravasation Key words: extravasation of cytostatics, vesicants, irritants, specific measures This thesis deals with the issue of extravasation in intravenous administration of cytostatics in oncologic patients, as one of the most feared complication of cytostatic therapy, which may has a serious irreversible consequences for patients. Extravasation describes the unintended leakage of drug from vessel into surrounding tissues or the unintended instillation of drug directly into the perivascular tissues. The severity of tissue injuries depends on several factors, including the volume and concentration of the cytotoxic agent, the localization of extravasation, the sensitivity of the tissue and the vesicant potential of the cytotoxic agent. In order to avoid extravasation, the principles of safe drug use must be followed, and if extravasation occurs, the recommendations for the drug should be known and followed. This is the only way to prevent serious damage to the patient, or significantly reduce the consequences. The experimental...
Treatment of lower extremity peripheral artery disease
Juhász, Jan ; Hrdina, Radomír (advisor) ; Pourová, Jana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Jan Juhász Supervisor: Prof. MUDr. Radomír Hrdina, CSc. Title of diploma thesis: Treatment of lower extremity peripheral artery disease The lower extremities ischemia is a disease caused most often by atherosclerosis during which the lumen in lower limb arteries becomes narrow. Its prevalence is increasing, especially in the developed countries. The disease can be asymptomatic and symptomatic. The symptoms are very unpleasant and decrease patient's quality of life. Advanced stages of the disease may be life threatening. Therefore, it is vital to timely and correctly diagnose the illness. During the therapy, it is possible to use pharmacological as well as non-pharmacological procedures, and, preferably, a combination of the two types of treatment. The pharmacotherapy can be divided into several parts. The prevention of atherosclerotic complications makes use of preventive measures and antiplatelet therapy to reduce the cardiovascular risk. The symptoms therapy focuses on improving patients' quality of life by prescribing the vasoactive medications cilostazol, naftidrofuryl or pentoxifylin. The critical limb ischemia therapy uses prostaglandin analogues alprostadil, iloprost, limaprost or...
The effects of some flavonoids on isolated rat aortic rings
Fišrová, Martina ; Vopršalová, Marie (advisor) ; Pourová, Jana (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Farmacology & Toxikology Student: Martina Fišrová Supervisor: PharmDr. Marie Vopršalová, CSc. Title of diploma thesis: The effects of some flavonoids on isolated rat aortic rings Flavonoids are secondary plant metabolites that are profusely represented in nature. They are known for their wide range of effects and many of them have beneficial effects on the human body. Above all, they have anti-bacterial and anti-inflammatory properties and effects on cardiovascular system - they cause vasodilation. The aim of this diploma thesis was to investigate vasorelaxation effects of selected flavonoids from the group of flavonolignans - 2,3-dehydrosilybin A (DHS-A), 2,3-dehydrosilybin B (DHS-B) and metabolites, which are components of the silymarin complex found in the plant Silybum marianum (Asteraceae). The vasorelaxation potential of tested substances was verified in ex vivo conditions on isolated rat aortic rings. The effect of increasing doses of individual substances in precontracted aortic rings with intact endothelium was measured. From the measured values, the DRC curves were constructed and EC50 values were determined. The analysis of the results shows, that DHS-A (EC50 = 30,1 mol/l) had the most signifiant activity. Its...
The in vitro effects of selected fenolic substances on isolated rat vessels
Rajtmajerová, Iveta ; Pourová, Jana (advisor) ; Hrdina, Radomír (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Iveta Rajtmajerová Supervisor: PharmDr. Jana Pourová, Ph.D. Title of diploma thesis: The in vitro effects of selected fenolic substances on isolated rat vessels Silymarin flavonolignans are endowed with various aspects. Recently their hepatoprotective effect has been discussed widely as well as their positive influence on the cardiovascular system. This diploma thesis is concerned with the vasodilatory effects of silybin-A, silybin-B, silybin-A + B and isosilybin-A on rat aorta. The aim of this study was to test the vasodilatory effect of selected substances. The dependence of aortic relaxation on the increasing concentration of tested substances was measured. Isolated Wistar breed rat aorta was used. DRC curves were created and EC50 values were assessed from the obtained values of vessel tension. The results were evaluated subsequently. The vasodilator potential of silybin-A and isosilybin-A is comparable; hence the position of the substituents may be irrelevant in having any impact on relaxation of the vessels. Different vasodilatory effects of silybin-A and silybin-B have been observed thus, the relaxation could be stereoselective. The testing of the equimolar mixture of silybin-A and...
Vasorelaxant activity of selected metabolites of quercetine on isolated rat aorta
Horáčková, Markéta ; Vopršalová, Marie (advisor) ; Pourová, Jana (referee)
Charles University in Prague Faculty of Pharmacy in Hradech Králové Department of Pharmacology and Toxicology Candidate: Mgr. Markéta Horáčková Supervisor: PharmDr. Marie Vopršálová, CSc. Title of rigorous thesis: The vasorelaxing effect of selected quercetin metabolites on isolated rat aorta Quercetin with its metabolites belongs into wide group called flavonoids, which are polyphenolic secondary metabolites of plants. This work, that is part extensive research ongoing in Department of Pharmacology and Toxicology of Faculty of Pharmacy in Hradec Králové, is focused on selected metabolites of propionic acid. In theoretical part I included characterisation of flavonoid group and their pharmacological effect. I also described morphology of blood-vessels and mechanisms of regulation of systemic circulation, because this work is about effect on blood-vessels. In experimental part we observed vasorelaxation effect of selected metabolites on isolated thoracic aorta of strain Wistar rat first. We compared effects of tested metabolites with quercetin, which was also included in experiment. As the most efficient metabolite was found 3-(3-hydroxyphenyl)propionic acid (3-HPPA). Its vasorelaxing effect was about one order bigger than quercetin and its other tested metabolites. 3-HPPA was selected for the...
The effect of quercetin metabolite on the rat isolated arteries.
Rudišarová, Simona ; Vopršalová, Marie (advisor) ; Pourová, Jana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Simona Rudišarová Supervisor: PharmDr. Marie Vopršalová, CSc. Title of diploma thesis: The effect of quercetin metabolite on the rat isolated arteries This study is focused on the research of the effects of flavonol quercetin and its selected metabolites (3-(3-hydroxyphenyl)propionic acid and 3-hydroxybenzoic acid) on blood vessels in vitro. A standardized method of an isolated rat aorta was used. The aim of this study was to find out how substances act on the isolated aorta and if the effect of the effective metabolite is dependent on the presence of the endothelium in the vessel. After precontracting with noradrenaline, individual concentrations of the test substances were added to the aortic ring bath (final concentrations in the bath ranging from 10-7 to 10-3 M). From the measured values EC50 values (quercetin = 3,63 . 10-5 M, 3-(3-hydroxyphenyl)propionic acid = 3,21 . 10-6 M, 3-hydroxybenzoic acid = 4,38 . 10-5 M) were obtained and the corresponding DRC curves were constructed using S.P.E.L. Advanced Kymograph Software. The experiment was performed once again with quercetin and 3-(3-hydroxyphenyl)propionic acid on the endothelium-denuded vessel. According to the results is...
Pharmacotherapy of diabetes mellitus followed up in diabetes clinic
Bradová, Hana ; Pourová, Jana (advisor) ; Hrdina, Radomír (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Mgr. Hana Bradová Supervisor: PharmDr. Jana Pourová, Ph.D. Title of rigorous thesis: Pharmacotherapy of diabetes mellitus followed up in diabetes clinic This rigorous thesis describes current approach to therapy of type 1 and type 2 diabetes mellitus in adult patients. The theoretical part includes subchapters related to evaluation of diabetes mellitus compensation, non-pharmacological measures and especially pharmacological treatment of this disease. Additionally it sums up key information about drugs available on the Czech market (metformin, pioglitazone, sulfonylurea derivatives, repaglinide, acarbose, dipeptidyl peptidase 4 inhibitors, glucagon-like peptide 1 analogs, gliflozines, insulins and insulin analogs) and recommended therapy. Furthermore separate subchapter is dedicated to continuous subcutaneous insulin infusion with an insulin pump. The experimental part introduces therapy of the disease on sample of 100 patients (50 type 1 diabetic patients and 50 type 2 diabetic patients) in a selected diabetes clinic. In addition to the pharmacological treatment and its results, selected patient data (for example age at which a diagnosis was made, duration of the illness, some comorbidities...
Název v anglickém jazyce: Evaluation of the efficacy of quarternary acetylcholinesterase reactivators in vivo.
Mackurová, Michaela ; Vopršalová, Marie (advisor) ; Pourová, Jana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Performed at: University of defence in Brno, Faculty of Military Health Sciences in Hradec Králové, Department of Toxicology and Military Pharmacology Student: Michaela Mackurová Leader of diploma thesis: PharmDr. Marie Vopršalová, CSc. Supervisor: kpt. PharmDr. Vendula Hepnarová, Ph.D. Title of diploma thesis: Evaluation of the efficacy of quarternary acetylcholinesterase reactivators in vivo NPL belongs to compounds of organic phosphorus and they are very common cause of poisoning as organophosphorus nerve agent. They can be absorbed by various routes - inhalated, ingest or by transdermal penetration. The problem is ineffective therapy and there is still no broad-spectrum reactivator able to efficiently restore AChE activity after intoxication by various organophosphates that will penetrates into CNS. The main aim of this experiment was to determine and compare the reactivating efficacy of two newly developed oximes K869 and K870 with commonly used oximes K160 and HI-6 against intoxication of sarin. The activity of reactivation was determined by standard spectrophotometric Ellman's method with using male Wistar laboraty rats. The results were evaluated as percentage of reactivation...
The effect of quercetin metabolite on the rat isolated arteries.
Rudišarová, Simona ; Vopršalová, Marie (advisor) ; Pourová, Jana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Simona Rudišarová Supervisor: PharmDr. Marie Vopršalová, CSc. Title of diploma thesis: The effect of quercetin metabolite on the rat isolated arteries This study is focused on the research of the effects of flavonol quercetin and its selected metabolites (3-(3-hydroxyphenyl)propionic acid and 3-hydroxybenzoic acid) on blood vessels in vitro. A standardized method of an isolated rat aorta was used. The aim of this study was to find out how substances act on the isolated aorta and if the effect of the effective metabolite is dependent on the presence of the endothelium in the vessel. After precontracting with noradrenaline, individual concentrations of the test substances were added to the aortic ring bath (final concentrations in the bath ranging from 10-7 to 10-3 M). From the measured values EC50 values (quercetin = 3,63 . 10-5 M, 3-(3-hydroxyphenyl)propionic acid = 3,21 . 10-6 M, 3-hydroxybenzoic acid = 4,38 . 10-5 M) were obtained and the corresponding DRC curves were constructed using S.P.E.L. Advanced Kymograph Software. The experiment was performed once again with quercetin and 3-(3-hydroxyphenyl)propionic acid on the endothelium-denuded vessel. According to the results is...

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