National Repository of Grey Literature 22 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Comparison of activation lower limb muscules in slide turn and turn in telemark position
Pospíšilová, Eva ; Jindra, Matouš (advisor) ; Chrástková, Martina (referee)
Title: Comparison of activation of lower limb muscles in a slide turn and in a telemark position turn. Objectives: The aim of this theses is to compare the muscle activation of selected muscles of the lower limbs in the slide turn and in the telemark technique. Methods: The muscle activation of selected muscles was measured during skiing using surface EMG. All probands crossed the track in two ways. The first one was the telemark technique and the second one the middle slide turn technique. The received signal from each ride was processed and evaluated. The results of both rides were compared to each other. Results: We found differences in the order of muscle activation between telemark and slide turn. We did not find unequivocal motion for each technique, which we could be compared to each other. Each probe had a different individual order of muscle activation in each technique. Keywords: downhill skiing, telemark, EMG, muscle activation
The effect of nerolidol nad valencene on the efficacy of 5-fluorouracil in colon cancer cells
Pospíšilová, Eva ; Skálová, Lenka (advisor) ; Hanušová, Veronika (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Eva Pospíšilová Supervisor: prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The effect of nerolidol nad valencene on the efficacy of 5- fluorouracil in colon cancer cells The cancer diseases belong among the most serious diseases on the world. Cytostatics, which are used for treatment, have many side effects and there is a high occurrence of resistance. Therefore scientists are looking for appropriate substances for the combination therapy. Sesquiterpenes, secondary metabolites of plants, could be one of the options. The aims of this work were to study the influence of cytostatic 5-fluorouracil and two sesquiterpenes, nerolidol and valencene on the proliferation of intestinal cancer cells and to find out the influence of sesquiterpenes on antiproliferative effect of 5-fluorouracil. Cancer cell line SW-620 derived from the colorectal metastatic carcinoma was used for our experiments. Neutral red uptake tests were used to measure the number of viable cells. The obtained results showed, that the inhibition of proliferation of cells SW-620 by 5-fluorouracil was concentration and time dependent. Trans-nerolidol showed antiproliferative effect even in low concentrations, while valencene...
The usage of qi gong for training of perceiving body schema
Pospíšilová, Eva ; Pánek, David (advisor) ; Véle, František (referee)
Title: The usage of qi-gong for training of perceiving body schema Summary: The goal of the work is to prove the presence of alpha activity in the electroencencefalographic record throughout the duration of the exercise qi-gong with open eyes and closed eyes, and to evaluate changes in the distribution of the scalp alpha activity with native EEG before and after the exercise. The observed research file was created from five probands between the ages of twenty-seven to fifty-two, which all practiced qi-gong for a duration of at least twelve months. The results showed the presence of alpha activity during exercising qi-gong with closed eyes in four probands, and in three probands there was also a present alpha activity during the exercise of qi-gong with open eyes. Furthermore was proven that the change in distribution of alpha activity during exercise of qi-gong with open eyes was from parietooccipital regions going temporo-frontally in comparison with the exercise of qi- gong with closed eyes and native EEG before and after exercise. The acquired results support in literature the described change of generators of alpha activity localized in the deeper structures of the brain. This process is connected with the decreased activity of the cerebral cortex with an increase in the particular limbic structures....
Adenylate cyclase toxin of bacteria Bordetella pertussis: mechanism of potassium efflux from macrophages
Pospíšilová, Eva ; Mašín, Jiří (advisor)
The adenylate cyclase toxin-hemolysin (ACT or CyaA) is a key virulence factor of Bordetella pertussis, the agent of the human respiratory disease whooping cough. CyaA penetrates phagocytes expressing the CD11b/CD18 integrin and exhibits two different activities. One toxin conformer oligomerizes in cell membrane and permeabilizes it by forming small cation-selective pores. Another toxin conformer appears to act as monomer. It forms a calcium influx path in the membrane, concomitantly with translocating into cells the adenylate cyclase enzyme domain that binds calmodulin and catalyzes unregulated conversion of cytosolic ATP into cAMP. We show here that CyaA causes efflux of K+ from CD11b+ cells by a mechanism that requires binding of CyaA to integrin CD11b/CD18 and permeabilization of the cellular membrane by pore-forming conformer of CyaA. Intact CyaA and the enzymatically inactive CyaA-AC- toxoid unable to generate cAMP produced the same kinetics of K+ efflux showing that elevation or signaling of cAMP had no role in this activity. The truncated CyaA variant (CyaA-AC) devoid of its invasive AC domain failed to promote K+ efflux despite a normal pore forming activity on erythrocyte and artificial membranes. However, binding of the MAb 3D1, which recognizes the distal segment of the AC domain...
název v anglickém jazyce není uveden
Pospíšilová, Eva ; Paleček, Jiří (advisor) ; Kršiak, Miloslav (referee) ; Rokyta, Richard (referee)
Tissue injury leads to increased sensitivity to noxious and innocuous stimuli due to mechanisms of peripheral sensitisation of primary nociceptors and central sensitization of neurons in the spinal dorsal horns. The subpopulation of capsaicin - sensitive sensory neurons plays an important role in this process. The capsaicin - sensitive neurons express capsaicin TRPV1 receptors (transient receptor potential 1) on their peripheral and central terminals, local high concentration capsaicin treatment can induce regional destruction of these endings. It is well estabilished that TRPV1 receptors play the key role in neural transmission of nociceptive information and its modulation. The aims of this study were to investigate the role of capsaicin - sensitive primary afferent fibers and the involvement of peripheral and central TRPV1 receptors in the development of hypersensitivity after surgical tissue trauma, to test the effect of high concentration capsaicin and the specific TRPV1 antagonist treatment on postoperative pain and to enlighten the function of central TRPV1 receptors in the neural mechanisms of nociception. Using behavioral testing methods, the responses to mechanical (Von Frey filaments) and thermal stimuli (radiant heat source) were tested on the rat plantar incision model of surgical pain...
Adenylate cyclase toxin of bacteria Bordetella pertussis: mechanism of potassium efflux from macrophages
Pospíšilová, Eva ; Konopásek, Ivo (referee) ; Mašín, Jiří (advisor)
The adenylate cyclase toxin-hemolysin (ACT or CyaA) is a key virulence factor of Bordetella pertussis, the agent of the human respiratory disease whooping cough. CyaA penetrates phagocytes expressing the CD11b/CD18 integrin and exhibits two different activities. One toxin conformer oligomerizes in cell membrane and permeabilizes it by forming small cation-selective pores. Another toxin conformer appears to act as monomer. It forms a calcium influx path in the membrane, concomitantly with translocating into cells the adenylate cyclase enzyme domain that binds calmodulin and catalyzes unregulated conversion of cytosolic ATP into cAMP. We show here that CyaA causes efflux of K+ from CD11b+ cells by a mechanism that requires binding of CyaA to integrin CD11b/CD18 and permeabilization of the cellular membrane by pore-forming conformer of CyaA. Intact CyaA and the enzymatically inactive CyaA-AC- toxoid unable to generate cAMP produced the same kinetics of K+ efflux showing that elevation or signaling of cAMP had no role in this activity. The truncated CyaA variant (CyaA-AC) devoid of its invasive AC domain failed to promote K+ efflux despite a normal pore forming activity on erythrocyte and artificial membranes. However, binding of the MAb 3D1, which recognizes the distal segment of the AC domain...
Nursing practices internal medicine in context with the development.
POSPÍŠILOVÁ, Eva
Nursing methods are understood as a collection of knowledge and instructions about how to perform nursing in order to achieve the most effective nursing care. It's not a set of fixed rules. Their development relies on the development of medicine and technology. They are in accordance with medicinal and technological knowledge. The goal of this thesis is to map the development of certain nursing methods in internal departments from the second half of the 19th century to the present day. It has been a qualitative research carried out by studying and analyzing written documents. This thesis was based on searching, sorting and consequent processing of historical archived publications and contemporary professional sources related to this topic. The thesis is divided into two parts. The first part deals with the history of nursing. It contains a brief description of the beginning of nursing along with the factors which have influenced nursing. Foundations of modern nursing have formed thanks to Florence Nightingale, who also influenced the development of nursing in the Czech lands. The history of Czech nursing is mostly concentrated around the period of the world wars. The second part is dedicated to specific nursing activities concerning healthy living spaces, rooms for patients, patients? beds and their modifications, care of patients? hygiene and monitoring the vital functions. The timeframe begins at the first half of the 19th century due to the establishment of real nursing professions at that time. Individual stages are ten years long. They indicate complex development of specific nursing methods from their beginnings to the present day. Mapping the historical development of certain nursing methods can contribute to the modern nurse's work. Past knowledge can help them to avoid mistakes and use of outdated methods. My thesis may also lead to a reflection of current methods, which may be replaced by more advanced ones.

National Repository of Grey Literature : 22 records found   1 - 10nextend  jump to record:
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26 POSPÍŠILOVÁ, Eva
13 Pospíšilová, Eliška
2 Pospíšilová, Evženie
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