National Repository of Grey Literature 15 records found  1 - 10next  jump to record: Search took 0.00 seconds. 
The role of adrenergic signaling in cardioprotection induced by adaptation to chronic hypoxia
Hahnová, Klára ; Novotný, Jiří (advisor) ; Polák, Jan (referee) ; Babula, Petr (referee)
Cardiovascular diseases are currently one of the most common causes of morbidity and mortality in the Western world. Adaptation to chronic hypoxia can contribute to the improvement of ischemic tolerance of myocardium but exact molecular mechanisms leading to the development of a cardioprotective phenotype are still unclear. There are some indications that point to the possible role of β-adrenergic signaling in these processes. In the first part of the thesis, we examined the effect of protective continuous (CNH; 24 h/day) and nonprotective intermittent (INH; 23 h/day hypoxia, 1 h/day reoxygenation) normobaric hypoxia on β-adrenergic signaling in the right (RV) and left ventricles (LV) of Wistar rats. Both hypoxic models led to decrease in the number of β1-adrenergic receptors (β1-ARs) in the RV. There were no significant changes in β-ARs in LV preparations. Although adenylyl cyclase (AC) activity stimulated through Gs proteins was decreased in the RV and increased in the LV after adaptation to CNH and INH, there were no significant changes in the expression of dominant AC 5/6 isoforms. Expression of Gs proteins was decreased in RV in both hypoxic models. These results suggest that chronic normobaric hypoxia may have a strong effect on myocardial β-adrenergic signaling without differences between...
The role of mitochondrial genome in cardioprotection induced by the adaptation to chronic hypoxia
Nedvědová, Iveta ; Žurmanová, Jitka (advisor) ; Polák, Jan (referee) ; Čížková, Dana (referee)
Cardiovascular intervention studies are a very important issue given that the ischaemic heart disease is one of the main mortality and morbidity causes in the Western world. Cardioprotection is mediated through a variety of signalling pathways in the cell that may directly or indirectly affect energy metabolism and mitochondria. Ischaemia-reperfusion injury of the heart significantly affect mitochondrial function revealing a potential therapeutic target. The role of mitochondria in the myocardium is not only in the field of energy homeostasis, but also in mediating the cellular response to reduced oxygen supply and in apoptosis regulation. This thesis aims to elucidate the response of the hypertrophied heart of the spontaneously hypertensive rat (SHR) and the derived conplastic strain with mitochondrial genome of normotensive Brown Norway (SHR-mtBN ) to the cardioprotective regime of adaptation to chronic normobaric hypoxia (CNH, Fi 0.1). The adaptive changes were studied at the cellular, protein and gene levels using Real-time RT-PCR, Biomark Chip Analysis, Western Blot, spectrophotometric measurements of enzyme activity and quantitative immunofluorescence analyses. The present thesis was based on a different cardioprotective phenotype between SHR and SHR-mtBN strains, i.e. a significantly smaller...
Significance of virtue in the philosophy of Plotinus
Polák, Ján ; Svoboda, David (advisor) ; Sousedík, Prokop (referee)
The place of virtue in the philosophy of Plotinus Abstract The main intention of this thesis is to clarify some aspects of Plotinus's concept of virtue. Significant part of it is a report of J. M. Dillon's article An ethic for the late anthique sage and its comparison with P. Hadot's essay Plotinus or the simplicity of vision, which form a base for characteristics of a dualism between sensual and spiritual world, the body/soul polarity, and relation between higher and lower virtues. Consequently a relationship of a Plotinian sage towards others is being investigated. The result of this thesis confirms that Dillon's interpretation of a radical distinction between polarities mentioned above is exaggerated and his pronouncement of an absence of the element of concern for others in Plotinus's ethical reflextion is basically mistaken. Key words Plotinus, ethics, virtue, neoplatonism, J. M. Dillon, P. Hadot, higher and lower virtues, relation with other, body and soul, sensual and spiritual world, late anthique philosophy.
The use of magnetic resonance spectroscopy for studying glucose metabolism
Kratochvílová, Simona ; Pelikánová, Terezie (advisor) ; Štich, Vladimír (referee) ; Polák, Jan (referee)
Magnetic resonance spectroscopy (MRS) is a noninvasive technique that enables to follow metabolic processes in selected tissues in vivo. Recently the attention has been focused on metabolic mapping in target organs of insulin action to describe the pathophysiology of insulin resistance. The aim of our study was to present the practical application of ³¹P (phosphorus) MRS and ¹H (proton) MRS in metabolic studies of skeletal muscle in insulin resistant subjects and in subjects with impaired fasting glucose. The third study was aimed to evaluate the brain metabolism with ¹H MRS in healthy controls and subjects with type 1 diabetes during hyperinsulinemia. ¹H and ³¹P MRS were performed using a MR Scanner Siemens Vision operating at 1,5 Tesla. To assess the parameters of glucose metabolism and insulin action oral glucose tolerance test and hyperinsulinemic euglycemic clamp were performed. The study 1 was aimed to evaluate the skeletal muscle (m. soleus) energetic metabolism in the offspring of hypertensive parents (OH) with a higher level of insulin resistance. The concentrations of selected high energy phosphates (phosphocreatine, inorganic phosphate, adenosintriphosphate, phosphomonoesters, phosphodiesters) were evaluated with ³¹P MRS. Their amount in OH was comparable to healthy controls. However we...
The Use of Scent in Marketing Communication
Polák, Jan ; Halada, Jan (advisor) ; Klabíková Rábová, Tereza (referee)
This bachelor thesis study sense of smell as mean to enhance marketing communication. In theoretical part of the thesis I focused on function of smell on biological level. I paid extraordinary attention to the brain and its function. I explained how the brain works and especially how the scents are being perceived. It is shown how important i tis to understand this topic well. The smell signals go directly to the deepest and oldest parts of our brain called the limbic system. This is where our emotions and memory are being processed. This tight connection between the smell, emotions and memory in some of the most important pieces of information that allows us to use the scent perception in marketing praxis. In the theoretical part of the thesis I mention also psychological effects of the smell such as the Proust phenomenon. This rather poetic term describes an unexpected feeling or long forgotten memory coming on our minds after exposure to certain smells that we have connected with our past experience. The practical part of the thesis is rather descriptive. I cited number of studies from scientific magazines which deal with effects of scents on human minds, perception and emotions, especially connected with practical use in marketing field. I added also some examples of the real companies which already use...
Functional genomic and pharmacogenomic analysis of metabolic syndrome aspects
Krupková, Michaela ; Šeda, Ondřej (advisor) ; Haluzík, Martin (referee) ; Polák, Jan (referee)
Metabolic syndrome is a prevalent disease characterized by concurrent manifestation of insulin resistance, obesity, dyslipidemia, hypertension and other hemodynamic and metabolic disorders. It has multifactorial type of inheritance and its resultant phenotype is determined by both environmental and genetic factors as well as their interactions. That is the main reason why comprehensive analysis of the genetic component of this syndrome is complicated in human population. Genetically designed experimental animal models are significant tools for analysis of genetic architecture of human complex conditions including the metabolic syndrome. The aim of this Thesis is utilization of functional and comparative genomic tools to uncover pathogenesis of metabolic syndrome aspects and their genetic determinants. We also studied pharmacogenetic interactions of these genetic determinants with drugs affecting particular components of the metabolic syndrome. Establishing and utilizing several genetically designed congenic rat strains, we undertook four different research projects focusing on pharmacogenetic interaction of all-trans retinoic acid and ondansetron with differential segment of rat chromosome 8, pharmacogenetic interaction of differential segment of rat chromosome 4 and dexamethasone, determining Plzf...
The concept of beauty in Ficino's commentary De amore and its influence on Renaissance aesthetics
Polák, Ján ; Dykast, Roman (advisor) ; Dadejík, Ondřej (referee)
The aim of this study is to examine piece of work of Marsilio Ficino known as De amore according to the characteristic of author's life and work, and to demonstrate its influence on Renaissance aesthetic. The main motive of the writing that was composed as commentary of Plato's Symposion is the function of love in the cosmology inspired by neoplatonists, mainly Plotinus. Ficino's principal contribution to the Renaissance and modern thinking is translation and development of Plato's work including his conception of double love. This study summarizes Ficino's life and work, afterward it examines writing itself emphasizing aesthetic themes and then demonstrates the influence of Ficino's conception of love on selected works of Renaissance literature. The selection of texts exposes transformation of this conception from piece of work directly inspired by Ficino's theory passing through writing in which is this theory confronted with formal literary requirements to the texts demonstrating dominance of literary form over philosophical content. Keywords Marsilio Ficino*Renaissance Platonism*Renaissance aesthetic*platonic love
Role of adipose tissue in the ethiopatogenesis of insulin resistance
Polák, Jan ; Štich, Vladimír (advisor) ; Hainer, Vojtěch (referee) ; Kazdová, Ludmila (referee) ; Rossmeisl, Martin (referee)
Adipose tissue is involved in etiopathogenesis of insulin resistance and subsequent metabolic disorders including type 2 diabetes mellitus and atherosclerosis. Mechanisms responsible for this association are investigated vigorously. One of the well accepted mechanisms linking excessive accummulation of adipose tissue with a development of insulin resistance are free fatty acids, which are released into circulation after hydrolysis of triglycerides stored in adipose tissue in a process of lipolysis. Impairments in a regulation of lipolysis are described in obese patients and lead to increased plasma level of free fatty acids and to impaired mobilisation of energy stores during exercise. In this PhD thesis, regulation of lipolysis in subcutaneous abdominal adipose tissuewas investigated using microdialysis technique. In the first paper we have observed that dysregulation in catecholamine-induced lipolysis and impaired insulin action in adipose tissue can be improved by dynamic-strength training in obese men. In the second study, we have demonstrated significant involvement of newly discovered lipolytic pathway mediated by atrial natriuretic peptide in lipolysis stimulation during exercise. Adipose tissue is producing several protein substances with regulatory and endocrine functions collectively named...
Epidemiological characteristic of viral hepatitis A in the Czech Republic
Polák, Jan ; Kynčl, Jan (advisor)
The aim of theses is to describe epidemiological characteristics of viral hepatitis A (VHA) in Czech Republic. The identification of specifics related to an increase of VHA and the importance of prevention and spread of this disease is examined The focal point of increased incidence of VHA are specific high risks groups mainly drug users. The general decrease in VHA infections among populations at large results in an increasing number of people having no antibody against VHA. The identified groups then become significant sources of pools of new infections affecting the rest of healthy population having no immunity against VHA virus. The previous epidemics of VHA during the second half of 20th century show the importance of state legislations or local rules governing public hygiene. These include regular controls of food producers, retailers and sources of drinking water. These measures by state and local health inspectors are essential to prevent large scale outbreaks of VHA epidemics. The study puts heightened emphases on health precautions while traveling in countries with a high prevalence of VHA. The preventative measures against VHA virus are important because the specific treatment to cure an illness does not exist and susceptibility to become infected with a virus is high. The personal hygiene such...
Adipokines (leptin and adiponectin) as predictors of weight loss and weight regain during one year period following the 10-week low caloric diet (NUGENOB study)
Drahoš, Jan ; Polák, Jan (advisor)
In the Czech republic, about 20% of women and 16% of men are obese. The key role in the etiology of obesity plays the imbalance between energy intake and expenditure, which is influenced by many factors, including leptin and adiponectin. Leptin is secreted mainly from adipocytes and it's levels increase with cell fat content and body fat percentage. Leptin itself is supposed to lead to weight loss, both by decreasing appetite and increasing energy expenditure. But high levels of leptin can be a signal of so called leptin resistance, a state in which leptin is losing it's effects. Adiponectin is secreted exclusively from adipose tissue. In obese people, lower levels of adiponectin were proven (i.e. adiponectin levels are in a negative correlation with body fat percentage). That's consistent with the fact that adiponectin is affecting a range of metabolic prosesses and thus improves insulin sensitivity and metabolic profile. For evaluation, we used a database of almost 750 subjects, that was build under the Nugenob study, conducted in 8 cities in 7 european countries. Al the participants were measured their anthropometric and biochemical parameters at the baseline and after 10 weeks of a standardized weight reduction program. The czech center retested the patient once again 1 year after the initial...

National Repository of Grey Literature : 15 records found   1 - 10next  jump to record:
See also: similar author names
12 POLÁK, Jan
17 POLÁK, Jaroslav
2 Polák, Jakub
12 Polák, Jan
2 Polák, Jaromir
17 Polák, Jaroslav
3 Polák, Jiří
4 Polák, Josef
1 Polák, Jozef
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