National Repository of Grey Literature 6 records found  Search took 0.01 seconds. 
Preparation and evaluation of new potential antimicrobial drugs and prodrugs
Imramovský, Aleš ; Vinšová, Jarmila (advisor) ; Kríž, Miroslav (referee) ; Pazdera, Pavel (referee)
5. SUMMARY This thesis deals with the synthesis of antimicrobial active compounds from the group of salicylanilide derivates that were found to be highly antituberculosis active compounds against atypical and resistant strains of Mycobacterium tuberculosis. These derivates served as the starting substances for the synthesis of prodrugs of acetic acid and amino acid esters, with the aim to reduce toxicity and to optimize physico-chemical properties and transport to the site of action. The second group under investigation was new modifications of some contemporary antituberculosis drug (isoniazide and pyrazinamide), the combination of either of these two compounds with second active molecule through an easily splitting methane bridge, which together could serve as depot forms, after release of both parts they may act as synergists. The outcomes of a literary search, that forewent synthesis, were published as survey articles in scientific papers. They deal with: a) cyclization forms of prodrugs (paper I), b) salicylanilides and their research trends (paper II) and c) isoniazide, its mechanism of activity and structure modifications published till now (paper V). The main scope of the thesis is experimental parts whose results were parcially published and belong to enclosed articles (paper III, IV and VI)....
Marketing Research
Veselý, Radek ; Pazdera, Přemysl (referee) ; Šimberová, Iveta (advisor)
This thesis lays emphasis on the satisfaction of the customer and building up relationship between the company and the customer. This thesis contains measurement of customer satisfaction with products and services offered by company CIPRES FILTR BRNO, Ltd. and the improvement suggestion of these services. Objection of marketing research is increase competitive advantage of company and attain better stability on extended industry market.
Simple route of caspase-3 FRET sensor synthesis using “click chemistry”
Lišková, Marcela ; Křenková, Jana ; Klepárník, Karel ; Pazdera, P. ; Foret, František
Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e.g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detected by spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on easily prepared Förster Resonance Energy Transfer (FRET). We use very simple chemistry called “click”. This type of chemistry takes advantages of quickness, simplicity and cheapness. “Click chemistry” is based on usage of various functional group and cross-linkers to combine individual molecules together.
Multi-step synthesis of caspase-3 sensor based on Förster resonance energy transfer
Lišková, Marcela ; Klepárník, Karel ; Pazdera, P. ; Foret, František
Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e.g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detected by spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on Förster Resonance Energy Transfer (FRET) to determine caspase-3 in cell nucleus or cytoplasm by apoptosis for very fast analysis by fluorescence microscopy without sample destroying.
Caspase 3 chemiluminiscence activity determination in apoptotic cells and design of Caspase 3 sensor
Lišková, Marcela ; Klepárník, Karel ; Pazdera, P. ; Foret, František
Apoptosis, or programmed cell death, is an essential physiological process that plays a critical role in development of tumor or autoimmune disorders. In some instances knowledges of this pathway can be used for inhibit or prevent cancer development and other diseases. One of the earliest and most consistent observed features of apoptosis is the induction of a series of cytosolic proteases – caspases.

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1 Pazdera, Petr
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