National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Evaluation of adjuvant treatment of malignant melanoma by real time RT-PCR
Gkalpakiotis, Spyridon ; Arenberger, Petr (advisor) ; Resl, Vladimír (referee) ; Pánková, Růžena (referee)
Melanoma is one of the tumors, characterized by considerable heterogeneity of expression of tumor-specific protein. Monitoring two or more markers can significantly increase the efficiency of detection. In our study, we concentrated to 5 marker: Melan-A/MART-1, gp 100, MAGE-3, MIA and Tyrosinase. Another potential tumor marker was telomerase. The most sensitive marker of progression proved to MAGE-3 (17 of 18 patients), followed by the positive marker gp100 (10 of 18 patients), MIA (9 of 18) and Tyrosinase (1 of 18). The Melan-A there was no statistically significant increase over the cutoff for all monitored patients with progression. Tyrosinase as a marker for circulating melanoma cells used in the past most frequently. Her role as a marker is highly debated and varies in different publications. For example The effectiveness of investigations Tyrosinase as the only marker of progression ranged from 6% to 59%. The reason may be technical error, a high percentage of false positives such as because of contamination or reduce expression Tyrosinase in advanced stages, which is associated with reducing the differentiation of tumor cells and decrease tumor melanization. Given that our work with progressive disease, most often found at the same time three positive markers, supports the detection of several marker...
Skin equivalents in basic and applied research: Development of human hair equivalents
Havlíčková, Blanka ; Arenberger, Petr (advisor) ; Pánková, Růžena (referee) ; Salavec, Miloslav (referee) ; Vízek, Martin (referee)
In this study, three novel organotypic human folliculoid assays for basic and applied hair research were developed. Two organotypic "sandwich" systems consist of a pseudodermis (collagen I mixed with and contracted by human interfollicular dermal fibroblasts) on which one of two upper layers is placed: either a mixture of Matrigel™ and follicular dermal papilla fibroblasts (DPC), with outer root sheath keratinocytes (ORSK) layered on the top ("layered sandwich" system), or a mixture of Matrigel™, DPC and ORSK ("mixed sandwich" system). Third, the patented new technique generating human folliculoid microspheres (HFM), consisting of human DPC and ORSK within an extracellular matrix was established. Studying a number of different markers (e.g. proliferation, apoptosis, cytokeratin- 6, versican), it was shown that these folliculoid systems, cultured under well-defined conditions retain several essential epithelial-mesenchymal interactions characteristic for human scalp hair follicle. Selected, recognized hair growthmodulatory agents modulate these parameters in a manner that suggests that all developed organotypic systems allow the standardized pre-clinical assessment of test agents on relevant human hair growth markers under substantially simplified in vitro conditions that approximate the in vivo situation....

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2 Pánková, Radka
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