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Bacteriophage 936 by qPCR quantification in dairy products.
Olša Fliegerová, Kateřina ; Mrázek, Jakub ; Štrosová, Lenka ; Němečková, I. ; Kopečný, Jan
Virulent bacteriophages are still representing significant threat in dairy production even with frequent disinfection and sterilization all the equipment in dairy plants. Therefore a reliable \nmethod of phage monitoring and quantification necessary part of phage prevention. Method is based on qPCR amplification and fluorescent detection of copies number of the phage main\nstructural protein and therefore is independent on the phage cultivation. It is quick, reliable a sensitive enough to estimate phage copy numbers in dairy starters and whey in any phase of dairy production. \n

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