National Repository of Grey Literature 320 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Recombinant expression of transcription factor TEAD1.
Řeháková, Jana ; Novák, Petr (advisor) ; Bláha, Jan (referee)
TEAD1 is one of the members of the TEAD transcription factor family. This transcription factor is very important, for example for development of organs. The structure of the entire TEAD1 transcription factor is not now yet known. Nevertheless, structure of two important domains are known. The TEA binding domain, which is important for the binding of the transcription factor TEAD1 to DNA, and the transactivation domain, to which coactivators bind. TEAD1 binds to DNA and upon coactivator bind it affects the transcription of various genes. Genes, that are affected by the transcription factor TEAD1 includes genes regulating proliferation, differentiation and apoptosis of cells. TEAD1 is also the target of the Hippo signalling pathway, which is active in adulthood and prevents abnormal growth of organs. Important for the activity of transcriptional factor TEAD1 are post-translation modifications, such as palmitoylation and phosphorylation. To discover the entire structure of the transcriptional factor TEAD1 and the way it interacts with DNA, the transcriptional factor TEAD1 was prepared recombinationally by expression in cells of Escherichia coli bacteria. Suitable conditions for production of the transcriptional factor TEAD1 were found and the cleavage of the histidine tag by thrombin was performed....
Company Computer Network Design
Novák, Petr ; Jordán, Vilém (referee) ; Ondrák, Viktor (advisor)
This bachelor thesis deals with computer network design in a company. The first chapter is about the theoretical concepts important for designing a computer network. The second chapter contains the analysis of a current status, which serves as a foundation for my own design solution, which is also the last chapter of this bachelor thesis. The last chapter also includes economic evaluation of the whole network design.
The Proposal of Customer Satisfaction Enhancement of Plzensky Dvur Restaurant
Marková, Denisa ; Babák, Dalibor (referee) ; Novák, Petr (advisor)
This bachelor´s thesis is concerned with the analysis of customer satisfaction of the restaurant Plzeňský dvůr. In the theoretical part it is concerned with the explanation of the fundamental terms which connect with these problems and methods of satisfaction recognition. In the practical part there is customer satisfaction determined by marketing research, which is based on enquiries through questionnaires. The result of this work is out put in the form of suggestions and recommendations, which should lead to an increase in the present level of customer satisfaction.
Customer Satisfaction Analysis and Recommendation for the Improvement
Benedělová, Martina ; Těšík, Jindřich (referee) ; Novák, Petr (advisor)
The object of this bachelor thesis is to increase customer satisfaction in Hranice Cementary Plant, a.s. on the market of cementary products especially with dry coat - plasters and mortary mixtures and to bring in a new product - sanation plasters to the market. The company has a place in a very special cementary sector, where the company has a limited number of producers, and where takes place the strong rival push. From this point of view there is a need to understand the conditions of this sector to help the company to be successful. In this bachelor thesis I am going to analyse a customer satisfaction and competitive position on the market of this company from the reason of bringing a new product onto the market.
Assessment and Proposal of Modernization of Selected Parts of Information System
Novák, Petr ; Dydowicz, Petr (referee) ; Klusák, Aleš (advisor)
The master’s thesis is focused on the assessment of the current state of the selected parts of the information system Zenit at company Nuvia a.s. and the proposal of their modernization. In individual parts of the thesis, there is defined theoretical basis, the assessment of the current state and the proposal of innovation of the current state.
Comparison of the effectiveness of online advertising channels demonstrated on an example of printed cookbook sale
Novák, Petr ; Máchová, Eva (advisor) ; Shavit, Anna (referee)
This bachelor thesis deals with a comparison of the effectiveness of advertising tools available online. The theoretical part addresses characteristic features of the work of the advertising channels studied and presents the Google Analytics traffic measurement tool, along with those functions that allow a deeper understanding of the equivalence of different sources of traffic on the conversions performed. The practical part compares paid advertising channels - Sklik, Google Adwords and Facebook ads - with the organic search results. The thesis explores user behaviour on websites, with an emphasis on the effectiveness of the campaign as expressed by conversion indicators. Using segmentation, the results obtained are compared in terms of age group, location, gender or the type of device used. In the final evaluation, the author places the results of the research into an overal context, emphasising the interplay between different marketing activities and analysing the influences which play a role in the effectiveness evaluated.
Recombinant preparation of DNA binding domain of transcription factor TEAD1
Kúdelová, Veronika ; Novák, Petr (advisor) ; Dračínská, Helena (referee)
TEAD proteins belong to a significant family of transcription factors that contribute to the regulation of organism growth and cell differentiation during its development by activating the expression of a wide variety of genes. This family shares two highly conserved sites, the TEA DNA binding domain, after which the proteins have been named, and the domain by which transcription factors bind other coactivators. Because TEAD proteins are not able to activate transcription themselves, they interact with a number of coactivators. These coactivators allow the transcription of the gene of interest to be regulated. Failure of TEAD protein activity regulation can lead to cancer. Therefore, TEAD family proteins nowadays play an important role in the development of new anticancer drugs. One way of inhibiting these proteins is to block the active site in their DNA binding domain, thus, to block their binding to DNA. This bachelor thesis deals with recombinant expression of said DNA binding domain of transcription factor TEAD1, which is extended by amino acids in unstructured regions. After finding suitable conditions of protein production, we proceeded to large volume production which was followed by purification and protein identity verification. Finally, the ability of the produced protein to interact...
Structure and dynamics of mouse C-type lectin-like receptors.
Wallenfels, Lucie ; Novák, Petr (advisor) ; Obšilová, Veronika (referee) ; Šebela, Marek (referee)
Natural killer (NK) cells represent indispensable part of the innate immunity as they are capable of promptly identifying virally infected or tumor cells and participating in the regulation of adaptive immune responses. These functions are ensured by the interplay between NK receptors, creating a complex regulatory system. Solving the receptors' structure may contribute to an overall understanding of NK cell biology. Presented thesis describes an elucidation of the structure of the inhibitory C-type lectin-like receptor (CTLR) Nkrp1b with an emphasis toward structural features (stalk, loop and oligomerization state) which might affect conformation or interactions of this receptor. The interaction of Nkrp1b with its ligand, Clr-b protein, is immunologically significant as it regulates NK cells' activity independently and monitors changes that are not visible to cytotoxic T lymphocytes. To study individual structural aspects of Nkrp1b, two protein variants were recombinantly prepared in bacterial expression system: entire ectodomain and ligand-binding domain lacking the stalk. Using a range of mass spectrometric techniques in combination with homology modeling and molecular dynamics, we proposed the Nkrp1b structure including its monomeric and dimeric arrangements. In addition, the oligomerization...
Multi-function Cultural Centre "Creative City" in Brno
Iermolovych, Ganna ; Novák, Petr (referee) ; Vojtová, Lea (advisor)
The master degree project presents design of conversion and extension of an old former prison situated between the streets Cejl, Soundni and Bratislavska for cultural, commercial and manufacturing purposes and forming multifunctional cultural center. The proposal consists of the conversion of two floors of existing building on the street Soudni, demolition of additional building on the street Bratislavska, while the additional building on street Cejl is belonging to other owner and will remain in existing status. Before the new building it is created a small square, that connects new and old building and the surroundings. Main goal is to create center for “creative” specialization, in which will be placed as working (studio, offices, conference hall) and performing spaces, but there also will be residential part (hostel and renting flats). Main purpose – create interesting space for collaboration of creative professions and to create the new cultural center.
Regulation of the activity of aspartic and serine proteases by selective natural inhibitors
Srp, Jaroslav ; Mareš, Michael (advisor) ; Novák, Petr (referee) ; Bařinka, Cyril (referee)
Proteases are involved in many physiological processes and their dysregulation is associated with various pathologies. Protease activity is effectively controlled by natural inhibitors. This PhD thesis is focused on the inhibitors of aspartic and serine proteases of animal and plant origin and provides the identification, biochemical characterization and structural description of their inhibition mechanisms. Plant Kunitz inhibitors are produced as defensive proteins, and they are able to block activities of a broad spectrum of proteases. In this thesis, the digestive proteolytic system of the Colorado potato beetle, a herbivore pest of potato plants, was described with the help of functional proteomics. It was shown that aspartic and serine proteases from this herbivore are effectively blocked by two potato Kunitz inhibitors (namely PCDI, PSPI). Using structural analysis, novel types of reactive centers were identified on PCDI and PSPI molecules for the inhibition of aspartic protease cathepsin D and the serine proteases trypsin and chymotrypsin. The analysis of the reactive center on a PCDI with the crystal structure of digestive cathepsin D from the Colorado potato beetle explained the mechanism of their interaction. Sphingolipids were identified as the first endogenous inhibitors of human...

National Repository of Grey Literature : 320 records found   1 - 10nextend  jump to record:
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