Národní úložiště šedé literatury Nalezeno 4 záznamů.  Hledání trvalo 0.00 vteřin. 
Metabolic rhythms of unicellular, nitrogen fixing cyanobacteria and possible interplay with modeled KaiABC circadian oscillator
Červený, Jan ; Nedbal, Ladislav
Circadian clocks of living organisms provide evolutionary advantage and make living in dynamic environment more efficient. The clocks affect human lives and health as well as control the simplest organisms such as prokaryotic cyanobacteria. The cyanobacteria represent an excellent model that is amendable to a multitude of genetic, biochemical, and biophysical methods. The cyanobacterium, Cyanothece sp. ATCC 51142 relies on the circadian clock to permit, in the same cell, anoxygenic nitrogen fixation at night and oxygenic photosynthesis during solar day. We measured real-time, in-situ photosynthetic and respiratory activities as well as the culture growth under light forcing conditions and also under constant light, i.e. free-running mode. Interestingly, the experiments show a strong 24h-period dynamic pattern that is replaced by apparent 12h-period in free running mode. The 24h-pattern does not change significantly when changing the light/dark ratio from 16hL/8hD to 12hL/12hD and 8hL/16hD. Furthermore, we tried to elucidate connection between these metabolic rhythms and known structure of KaiABC circadian oscillator by means of mathematical modeling. One of simulation results show a strong correlation between the presumed catabolic event indicated by significant peak in respiratory activity, and simulated dynamics of KaiB4 complex in modeled circadian pacemaker. A causal relationship between these 2 events is suggested because KaiB4 facilitates dephosphorylation of KaiC6 hexamer, which is known to signal upcoming dark period.
Plný text: Stáhnout plný textPDF
Seeing is believing
Govindjee, J. ; Nedbal, Ladislav
The authors present a short review of chlorophyll fluorescence imaging technique as used in plant science. The review is introducing a special issue of Photosynthetica journal dedicated to various applications of the method.

Viz též: podobná jména autorů
1 Nedbal, Lukáš
Chcete být upozorněni, pokud se objeví nové záznamy odpovídající tomuto dotazu?
Přihlásit se k odběru RSS.