National Repository of Grey Literature 12 records found  1 - 10next  jump to record: Search took 0.00 seconds. 
Solubilisation, Purification and Reconstitution of Human 17beta-hydroxysteroid dehydrogenase type 3
Zahradníková, Tereza ; Navrátilová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Tereza Zahradníková Supervisor: Prof. Ing. Vladimír Wsól, Ph.D. Consultant: RNDr. Lucie Zemanová, Ph.D., PharmDr. Adam Skarka, Ph.D. Title of rigorous thesis: Solubilisation, Purification and Reconstitution of Human 17beta-hydroxysteroid dehydrogenase type 3 Short-chain dehydrogenase/reductase superfamily is a large group of NADP(H)/NAD(H)-dependent oxidoreductases. Human SDR enzymes are classified into 47 families, including cytosolic and membrane-bound ones. One of them is a human membrane-bound enzyme 17β-hydroxysteroid dehydrogenase type 3 (17β-HSD3), which participates in the biosynthesis of steroidal hormones and mainly catalyses the conversion of androstenedione to testosterone. The aims of this study were to find a suitable detergent for successful solubilisation, to purify the enzyme and to prepare a reconstitution system for further study of the 17β-HSD3 enzyme. Microsomes containing overexpressed 17β-HSD3 were isolated from Sf9 insect cells (Spodoptera frugiperda). The first step was the solubilisation process, which involved detergent screening. Six detergents at different concentrations were tested: ASB 14-4, C12E8, DDM, CHAPS, Igepal CA-630 and Triton X-100. The detergent ASB...
The attitude of the elementary school pupils in Benešov to PE and sport activities.
Navrátilová, Hana ; Janák, Vladimír (advisor) ; Ruda, Tomáš (referee)
Title: The attitude of the elementary school pupils in Benešov to PE and sport activities Aim of the thesis: The main aim of this Bachelor's thesis is, firstly, to determine through a questionnaire the attitudes of pupils from primary schools in Benešov to physical education and sporting activity and later, based on the results obtained, make comparisons and propose certain changes with the intention of increasing the pupils' interest in sport. Methodology: All the data was obtained through a non-standard questionnaire, which was prepared for the purpose of this thesis. It predominantly contained closed and semi-closed questions. Only one question was open. The questionnaire was intended for pupils in the second grade of primary school. In addition, an oral interview, supplementing the written questionnaire, was undertaken. The data obtained were represented graphically and evaluated in writing. Results: The results from individual schools were mutually compared. From them it was determined that the attitudes to physical education and sporting activity are more or less the same for pupils from all three schools. The results from the research were additionally compared to formulated prerequisites, through which the important influence of parents on the participation in sport of their offspring and...
Unit owners association as a subject of law
Navrátilová, Hana ; Oehm, Jaroslav (advisor) ; Plíva, Stanislav (referee)
Unit Owners Association as a Subject of Law Objective of my thesis is to describe development of legal regulation of the Unit Owners Association, to analyse the current legal regulation of the Unit Owners Association in the Act No. 72/1994 Coll., on apartment ownership and to compare the current legal regulation with latest legal regulation in the new Civil Code. This topic is very live, because the issue of apartment ownership concerns more and more residents. The unit owner is also co-owner of common parts of a building. The Unit Owners Association is a legal entity determined for administration of common parts of the building. The thesis is composed of four chapters. Chapter One describes development of the legal regulation of the Unit Owners Association in the Act No. 72/1994 Coll., on apartment ownership, which was amended several times. Chapter Two analyses the current legal regulation of the Unit Owners Association mainly from the point of view of its legal capacity and internal arrangement. Legal capacity of the Unit Owners Association is restricted, because this legal entity is legally competent to execute rights and to commit itself only in the cases related to administration, operation and repairs of common parts of a building. This chapter consists of six parts which focused on formation of the...
Comparison of fitness centres in Benešov
Navrátilová, Hana ; Voráček, Josef (advisor) ; Šíma, Jan (referee)
Title: Comparison of fitness centres in Benešov Aim of the thesis: This bachelor thesis aims at comparing selected fitness centres in Benešov. Survey of quality of the services offered here was undertaken by means of the SERVQUAL questionnaire where customers expressed their views on the services provided by the centre, as well as on services expected in their "perfect" fitness centre. This questionnaire allowed for the comparison of the fitness centres and recommendation of certain changes which could lead to an improvement in quality and therefore also to increased satisfaction of the customers. Methodology: Quality of the services was compared by use of a standardized SERVQUAL questionnaire which was submitted to the clients of fitness centres in Benešov in spring 2013. The questionnaire reviewed and compared the expected quality of offered services which in the clients' opinion belonged to an ideal fitness centre, with the quality of services perceived by the clients in individual fitness centres. Results: The results of the analysis of customer satisfaction with the services offered by fitness centres in Benešov imply that the clients are most satisfied with services offered by "S- centrum". This centre has nevertheless also room for improvement, and to achieve full customer satisfaction the...
Václav (Remedius) Prutký OFM: Itinerarium missionum apostolicarum (introductory study, edition and translation with commentary on part I of the work)
Förster, Josef ; Svatoš, Martin (advisor) ; Hojda, Zdeněk (referee) ; Navrátilová, Hana (referee)
The dissertation presents primarily the text itself of part of Itinerarium (the first 31 chapters) in the form of a critical edition. It thus greatly eases the work of all future researchers in studying the work in the Latin original. The edition was acquired on the basis of a manuscript autograph, the property of the Franciscan Brothers Provenance in Prague kept in the National Library of the Czech Republic. The Itinerarium ranks amongst the most important works from a file of manuscript sources, the authors of which are members of the St. Wenceslas provenance of the order of lesser brothers of St. Francis, who were active during the course of the 18th century on missions in Egypt or Ethiopia and attained a prestigious standing in the mission hierarchy (Jakub Římař, Remedius Prutký, Kristián Schneider). Their works, which are of a various nature (itineraries, journals, collections of medical prescriptions, language dictionaries, liturgical manuals etc.), may be valuable sources for experts in a diverse range of fields: oriental studies, Egyptology, geography, history, linguistics, literary history etc. With regard to the fact that Prutký's literary work is unknown to the Czech reader and has been presented only thanks to the translation of two chapters acquired in the 1940s by Z. Kalista, it evidently...
Václav (Remedius) Prutký OFM: Itinerarium missionum apostolicarum (introductory study, edition and translation with commentary on part I of the work)
Förster, Josef ; Svatoš, Martin (advisor) ; Hojda, Zdeněk (referee) ; Navrátilová, Hana (referee)
The dissertation presents primarily the text itself of part of Itinerarium (the first 31 chapters) in the form of a critical edition. It thus greatly eases the work of all future researchers in studying the work in the Latin original. The edition was acquired on the basis of a manuscript autograph, the property of the Franciscan Brothers Provenance in Prague kept in the National Library of the Czech Republic. The Itinerarium ranks amongst the most important works from a file of manuscript sources, the authors of which are members of the St. Wenceslas provenance of the order of lesser brothers of St. Francis, who were active during the course of the 18th century on missions in Egypt or Ethiopia and attained a prestigious standing in the mission hierarchy (Jakub Římař, Remedius Prutký, Kristián Schneider). Their works, which are of a various nature (itineraries, journals, collections of medical prescriptions, language dictionaries, liturgical manuals etc.), may be valuable sources for experts in a diverse range of fields: oriental studies, Egyptology, geography, history, linguistics, literary history etc. With regard to the fact that Prutký's literary work is unknown to the Czech reader and has been presented only thanks to the translation of two chapters acquired in the 1940s by Z. Kalista, it evidently...
Egyptian historical thought: the visitors' graffiti of the New Kingdom at Saqqara and Abusir as a case study
Navrátilová, Hana ; Bareš, Ladislav (advisor) ; Baines, John (referee) ; Loprieno, Antonio (referee)
As an Egyptologist and historian I have tried to partner in this dissertation Egyptological and historical methods used on the topic of the uses of the past and on the issue of the presence of uses of the past in the Egyptian culture. There is a large discussion on these enquiries in both disciplines. I have focused on sources originating in the Egyptian New Kingdom (around 1540 to 1080 BC) and tried to analyse some issues related to the social memory and presumed historical awareness of this period. There is a specific material - a group of inscriptions called the "visitors' inscriptions" (Besucherinschriften). These texts are well-known to Egyptology but in my opinion deserve even more attention, being an attestation to the culture of a period literate stratum - the scribes. The thesis is divided into three major parts: 1/ the uses of the past - theoretical approaches, giving also the reasons for carrying out the analysis of the uses of the past of the ancient cultures, especially Egypt; 2/ the New Kingdom and presumed uses of the past in the Egyptian society of that time; 3/ the visitors' graffiti, including:  state of research, the graffiti database project;  the description of the graffiti in a statistical overview;  interpretations and conclusions, attempting also to insert the graffiti into the...
Strategic brand management of Kofola
Navrátilová, Hana ; Postler, Milan (advisor) ; Geschmay, Jan (referee)
Kofola shows a perfect example of a successful brand revival and its further development. It prooves, that brand's history and knowledge are just one of many factors contributing to bilding a strong brand. The most important is the whole process of effective and strategic brand management. This diploma thesis analyzes the strategic brand management of Kofola. Its goal is to reveal the main keys to success standing behind both the brand's and the company's results. At the same time, several imperfections and unused opportunities are uncovered and on their basis recommendations for improvement are made. Part of the thesis deals with an own marketing research, based on a questionnaire method. The questionnaire focuses mainly on perception of Kofola's image and the way of presenting it in the last summer campaign. Based on the questionnaire results there is a hypothesis examined that people perceive Kofola's "retro" image positively and that this way of communication is the right way also for the future.
Motivation of workers of specific production in a small firm
Navrátilová, Hana ; Kleibl, Jiří (advisor) ; Dvořáková, Zuzana (referee)
The thesis is dealing with the motivation of human resources in a small firm producing the specialized medical equipment. The aim is to analyse the actual situation of a motivation stucture and work satisfaction through the use of an empirical research and to suggest recommendations for the improvement of this area of the company's human resource management. The theoretical part characterizes a question of work motivation, system of assessment and remuneration of workers including employee benefits. The practical part of the thesis characterizes these areas of the personnel work in the company and then it analyses factors of motivation and work satisfaction of employees. Results of the questionnaire examination are used for the suggestions of some measures for the improvement of work motivation, satisfaction and stabilisation of the company's employees.

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2 Navrátilová, Helena
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