National Repository of Grey Literature 169 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Effect of glucose treatment on endoglin and biomarkers of endothelial dysfunction in endothelial cells
Adamcová, Adriana ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Effect of glucose treatment on endoglin and biomarkers of endothelial dysfunction in endothelial cells Author: Adriana Adamcová Supervisor of Diploma Thesis: prof. PharmDr. Petr Nachtigal, Ph.D. Background: The aim of this thesis was to evaluate the expression of endoglin and biomarkers of endothelial dysfunction (eNOS, VCAM-1) in aortic endothelial cells exposed to oscillating glucose. Methods: Human aortic endothelial cells (HAECs) were treated with alternating normal (5 mM) and high (25mM) glucose for 7 days. The control group, was treated with a constant level of normal glucose. Gene expression of endoglin, eNOS, VCAM-1, HIF1α, KLF6, RELA and LXR was measured by quantitative real-time PCR. Flow cytometry was used to detect endoglin and VCAM-1 protein levels. Results: Treatment with high levels of glucose resulted in significantly increased expression of endoglin, eNOS, VCAM-1, HIF1α, RELA and LXR by means of PCR. The flow cytometry method showed a significant difference in protein levels in both endoglin and VCAM-1. Conclusions: The results of this diploma thesis showed that oscillating glucose increases the expression of endoglin and other biomarkers of endothelial...
Effect of soluble endoglin on cholesterol metabolism in the liver of high-fat diet-fed transgenic mice.
Hofmanová, Kristýna ; Nachtigal, Petr (advisor) ; Prašnická, Alena (referee)
Kristýna Hofmanová Effect of soluble endoglin on cholesterol metabolism in the liver of high-fat diet-fed transgenic mice Diploma thesis Charles University, Faculty of Pharmacy in Hradec Králové Pharmacy Background: Increased plasma concentrations of soluble endoglin (sENG) are observed in metabolic deseases like diabetes mellitus type 2 or hypercholesterolemia. It has already been shown that sEng influence the cholesterol metabolism i healthy mice fed standard laboratory diet. However, the effect of sENG on cholesterol metabolism in combination with high fat diet (HFD) is not known yet. The aim of this diploma thesis was to elucidate the influece of the sENG on the meabolism of cholesterol in the liver of transgenic mice (Sol-Eng+ ), with liver demaged by NASH (Non-alcoholic steatohepatitis) induced by HFD. Methods: Three months old male mice (Sol-Eng+ ) with high plasma levels of sENG on the basis of the CBAxC57BL/6J and control "wild-type" mice with unspecified levels of soluble endoglin (n=8, in each group) we used in this study. The animals were divided into groups acording of the level of soluble endoglin and the type of diet: "wild-type" fed for six moths with standard laboratory diet (WTchow); "wild-type" with high fat diet (WTHFD) and high- plasma sENG group fed HFD for six months...
Effect of soluble endoglin on bile acids metabolism in the liver of high-fat diet-fed transgenic mice.
Jiroušková, Barbora ; Nachtigal, Petr (advisor) ; Schreiberová, Jolana (referee)
Barbora Jiroušková Effect of soluble endoglin on bile acids metabolism in the liver of high-fat diet-fed transgenic mice. Diploma thesis Charles University, Faculty of Pharmacy in Hradci Králové Pharmacy Background: Increased plasma concentrations of soluble endoglin (sEng) have been demonstrated in metabolic disorders such as hypercholesterolemia or insulin resistance. Metabolic diseases and inappropriate diet are the basis for the development of NASH (Non-alcoholic steatohepatitis). The aim of this diploma thesis was to determine how high plasma concentrations of sEng affect the bile acids metabolism in the liver of mice fed with high-fat diet, which induced NASH. Methods: Three-month-old male mice with CBAxC57BL/6J base (n = 8, in each group) were used in experiment: 1) "wild-type", which were fed a standard diet (WTchow) for 6 months; 2) "wild-type" male mice fed a high fat diet (cholesterol, glucose and fructose) (WTHFD); 3) males expressing human sEng (sENGHFD) which were fed a high fat diet, glucose and fructose. In vivo kinetic study was performed to determine bile flow. Expression of transport proteins responsible for bile acids transport was performed at mRNA and protein level by qRT-PCR and Western blot. Results: High levels of soluble endoglin did not significantly affect BA homeostasis...
Anomalies of visceral arteries
Lacušová, Monika ; Kudláčková, Zděnka (advisor) ; Nachtigal, Petr (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department Department of Biological and Medical Science Candidate Monika Lacušová Consultant PhDr. Zděnka Kudláčková, Ph.D. Thesis Title Anomalies of visceral arteries Introduction: Understanding of occurrence of visceral arteries anomalies is important for performing surgeries within abdomen. In case of lack of understanding of these anomalies, serious damage of blood vessels may occur, resulting in disorders of blood supply for liver, pancreas, as well as other organs located in abdomen. Early and accurate diagnostics is of high importance, and it may be performed via display methods, most frequently through computer tomography. Objective: The aim of this thesis is to evaluate occurrence and types of anomalies of visceral arteries through scans resulting from computer tomography. Methods: We have obtained the data from medical records of patients through hospital information system. These were the records of patients treated at the Surgery Clinic of the Faculty Hospital in Hradec Králové. Overall, there was a set of 430 patients included in the evaluation of the records taken between January 1, 2019 and December 31, 2019. It was essential, that the patients underwent the examination by computer tomography. Therefore, we ruled out 115...
Soluble endoglin: effect on bile acids and cholesterol transport in livers
Grešková, Mária ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
Maria Greskova Soluble endoglin: effect on bile acids and cholesterol transport in livers Diplomathesis Charles University in Prague, Faculty of Pharmacy in Hradec Kralové Pharmacy Background: Increased plasma levels of soluble endoglin (sEng) have been shown in patients with acute heart failure, hypertension, diabetes mellitus type 2, and other metabolic and cardiovascular diseases such as e.g. hypercholesterolemia, atherosclerosis and cystic fibrosis, which influence as well liver functions. As there are no available data showing effect of sEng on the transport of cholesterol and bile acids, the aim ofthis diplomathesis wasto describe them. Methods: In this study were used six months-old female mice with high level of sEng on CBAxC57BL/6J background and control mice (n = 8, in each group) fed with chow diet for three months. /n vivo study was performed to analyze bile flow. mRNA expression of proteins responsible for cholesterol and bile acids transport and metabolism in the liver was performed by qRT-PCR. Results: sEng did not lead to changes neither in the bile flow nor in the concentration of cholesterol in plasma and liver. There were found no changes in the mRNAexpression of Sr-b1, Hmgcr, Abcg5, Abcg$, Acat2, Abcb4, Abcb11, Abcc2. sEng led to decrease in mRNAexpression of Cyp7al. Conclusion:...
Evaluation of cell adhesion molecules expression in mice aorta in animal model of non-alcoholic steatohepatitis.
Matějková, Barbora ; Nachtigal, Petr (advisor) ; Doleželová, Eva (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Consultant: prof. PharmDr. Petr Nachtigal, Ph.D. Candidate: Barbora Matějková Title of Thesis: Evaluation of Cell Adhesion Molecules Expression in Mice Aorta in Animal Model of Non-alcoholic Steatohepatitis Background: The purpose of the thesis was to observe changes in the expression of ICAM-1 and VCAM-1 in mice aorta endothelium. The study compares results of a group of mice after the administration of a NASH inducing diet and a group of mice fed by a chow diet. Method: Two groups of C57BL/6 mice were used for this experiment. At the age of ten weeks the experimental group was transferred to a NASH inducing diet which contains 40 % of calories from fat and 0.2 % from cholesterol. In addition to this diet, the experimental group was fed by fructose and glucose dissolved in drinking water with the concentration of 42 g/l (called "FFC-type diet" - high saturated fat, high saturated fructose, high saturated cholesterol). This diet lasted for 25 weeks. The control group was fed by a standard diet for rodents (called "chow-type diet"). The obtained samples were analysed by immunohistochemical methods. For ICAM-1, the indirect three-step avidin- biotin complex method was used. VCAM-1 molecules were...
Hepatocyte ROCK1 kinase activity incites liver inflammation in murine non-alcoholic fatty liver disease
Dohnálková, Ester ; Nachtigal, Petr (advisor) ; Najmanová, Iveta (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Biological and Medical Sciences Candidate: Mgr. Ester Dohnalkova Consultant: prof. PharmDr. Petr Nachtigal, Ph.D. Title of Thesis: Hepatocyte ROCK1 kinase activity incites liver inflammation in murine non- alcoholic fatty liver disease Background: Rho-associated protein kinase 1 (ROCK1) activity has been previously implicated in lipotoxicity-induced extracellular vesicle (EV) release from hepatocytes. In turn, lipotoxic hepatocyte-derived EVs incite inflammatory response in monocytes and macrophages. In addition, we previously found that activated ROCK1 is a distinct histologic feature of inflammatory stage of murine and human nonalcoholic fatty liver disease (NAFLD). However, it is currently unknown whether activated ROCK1 may directly contribute to NAFLD pathogenesis. Therefore, we aimed to examine the effect of hepatocyte ROCK1 kinase activity on the development of inflammation in a model of murine NAFLD. Methods: To assess the role of ROCK1 in NAFLD, we have adopted an in vivo gain-of-function approach. C57BL/6J mice were injected with either: a) vehicle; b) adeno-associated virus, serotype 8 (AAV8)-packaged, TBG-driven constitutively active ROCK1 (CA-ROCK1) construct; or c) AAV8-packaged, TBG-driven kinase-dead...
Soluble endoglin role in the pathogenesis of endothelial dysfunction
Vitverová, Barbora ; Nachtigal, Petr (advisor) ; Vrablík, Michal (referee) ; Mladěnka, Přemysl (referee)
Charles University, Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Candidate: Msc. Barbora Vitverova Supervisor: prof. PharmDr. Petr Nachtigal, Ph.D. Title of Doctoral Thesis: Soluble endoglin role in the pathogenesis of endothelial dysfunction The endothelium is a major regulator of vascular homeostasis and exerts a number of vasoprotective effects, including production of vasodilating agents, suppression of smooth muscle cell growth, or inhibition of inflammatory responses. Most of these effects are mediated by nitric oxide (NO), the most effective endogenous vasodilator. Decreased NO production or its activity is a hallmark of endothelial dysfunction leading to an impaired endothelial-dependent vasodilation. Endothelial dysfunction is the first and key step in the development of atherosclerosis and can be detected before structural changes in the vessel wall. Atherosclerosis is a complex inflammatory disease underlying many cardiovascular disorders including ischemic heart disease and represents the major cause of morbidity and mortality in developed countries. Statins are drugs of choice in the treatment of atherosclerosis and ischemic heart disease, which have been shown to reduce LDL cholesterol levels and modulate endothelial function with their...
Soluble endoglin effects on endothelial dysfunction markers in mouse aorta
Kalmanová, Lenka ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Soluble endoglin effects on endothelial dysfunction markers in mouse aorta Author of Diploma Thesis: Lenka Kalmanová Supervisor of Diploma Thesis: prof. PharmDr. Petr Nachtigal, Ph.D. Background: The aim of the presented diploma thesis was to identify and subsequently evaluate the expression of endothelial dysfunction markers (ICAM-1, VCAM-1, P- selectin) in aortas of two groups of transgenic mice. The difference between control and experimental group was in the level of human soluble endoglin. Methods: 12 months old male mice of transgenic strain CBAxC57BL/6J with inserted genetic information for the expression of human soluble endoglin were utilized for the experiment. The mice were divided into two groups - the experimental group (high levels of sEng) and the control group (low levels of sEng) by ELISA analysis of levels of sEng in plasma. Both groups of mice were fed with standard laboratory rodent diet. Western blot analysis of adhesion molecules ICAM-1, VCAM-1 and P-selectin in mouse aorta and subsequently ELISA analysis of plasma levels of sVCAM-1 molecule were performed. Total cholesterol and triacylglycerol levels were demonstrated by biochemical analysis....

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