National Repository of Grey Literature 106 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
GENE EXPRESSION AND IMMUNOLOGICAL RESPONSE IN MICE EXPOSED TO ZnO NANOPARTICLES
Rössner ml., Pavel ; Vrbová, Kristýna ; Strapáčová, S. ; Rössnerová, Andrea ; Ambrož, Antonín ; Brzicová, Táňa ; Líbalová, Helena ; Javorková, Eliška ; Zajícová, Alena ; Holáň, Vladimír ; Kulich, P. ; Večeřa, Zbyněk ; Mikuška, Pavel ; Coufalík, Pavel ; Křůmal, Kamil ; Čapka, Lukáš ; Dočekal, Bohumil ; Šerý, Omar ; Machala, M. ; Topinka, Jan
We analyzed gene expression changes in the lungs and the immunological response in splenocytes of mice exposed by inhalation of ZnO nanoparticles - NP. Adult female ICR mice were treated for three days and three months, respectively. Analysis of differential expression in genes involved in oxidative stress was conducted using quantitative RT-PCR. The potential immunotoxic and immunomodulatory effects of ZnO NP were analyzed by phenotyping and cytokine production by splenocytes after three months exposure. Three days exposure resulted in down-regulation of GCLC, GSR, HMOX-1, NQO-1, NF-kB2, PTGS2 and TXNRD1 mRNA expression, three months exposure increased the expression of these genes. Three months exposure caused a significant decrease in the percentage of granulocytes in the spleen cells, and affected the production of IL-10 and IL-6 by lipopolysaccharide-stimulated leukocytes. In summary, our study revealed changes in the expression of genes involved in the oxidative stress response following acute ZnO NP exposure. Subchronic ZnO NP exposure induced immunomodulatory effects in the spleen.
Oxidative potetial of atmospheric aerosol as indicator of ROS formation
Barboříková, Hana ; Mikuška, Pavel ; Hegrová, J. ; Krajčovič, J.
Atmospheric particulate matter (PM) affects our environment especially human health because it contains many toxic chemical substances. The purpose of this study is to determine a total content and a soluble fraction of selected metals and also the contribution of metals soluble in pulmonary fluids to a total oxidative potential of PM.
Chemical composition of aerosol particles in emissions from coal combustion in different boilers used for residential heating
Křůmal, Kamil ; Mikuška, Pavel ; Horák, J. ; Hopan, F.
Organic compounds (polycyclic aromatic hydrocarbons, alkanes) and organic markers (hopanes) used for the identification of aerosol emission sources were measured in emissions from the combustion of hard and brown coal. Coal was combusted in different boilers (overfire boiler, boiler with down-draft combustion and automatic boiler) used for residential heating in the Czech Republic.
Effect of inhaled metal oxide nanoparticles on organs of exposed mice
Mikuška, Pavel ; Večeřa, Zbyněk ; Dočekal, Bohumil ; Čapka, Lukáš ; Křůmal, Kamil ; Coufalík, Pavel ; Moravec, Pavel ; Buchtová, Marcela ; Míšek, Ivan ; Vrlíková, Lucie ; Smutná, Tereza ; Dumková, J. ; Hampl, A. ; Fictum, P.
Adult mice were continuously exposed to metal oxide nanoparticles for different time intervals. At the end of experiment, mice organs (lung, brain, kidney, liver and spleen) were collected for chemical, biochemical, histological and electron microscopical analyses.
Determination of dicarboxylic acids in air by aerosol sampler
Čapka, Lukáš ; Mikuška, Pavel
Dicarboxylic acids (DCAs) are important components of water soluble aerosol compounds. Aerosol particles are quantitatively collected using a continous aerosol sampler, CGU-ACTJU, into deionized water. The ACTJU effluent is permanently sucked out from the sampler and on-line analysed for DCAs content by means of ion chromatography.
Regional Air Pollution Transport and Its Influence to Pollution Hotspot in th Czech Republic.
Kozáková, Jana ; Vodička, Petr ; Pokorná, Petra ; Ondráčková, Lucie ; Ondráček, Jakub ; Hovorka, J. ; Křůmal, Kamil ; Mikuška, Pavel ; Moravec, Pavel ; Schwarz, Jaroslav
The aim of the study was to find out the local effect and the regional air pollution transport effect on the air quality of the Ostrava Radvanice district, the problematic air pollution hot spot.
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Characterization of organic compounds in PM1aerosol in Kosetice and Brno in winter season 2017
Křůmal, Kamil ; Mikuška, Pavel ; Večeřa, Zbyněk
In this work, the organic compounds and organic markers used for the identification of sources of aerosols were measured. Monosaccharide anhydrides and resin acids (emissions from biomass combustion) and polycyclic aromatic hydrocarbons (emissions from traffic and incomplete combustion) were observed especially. Hopanes, steranes (traffic, coal combustion) and alkanes were next groups of analysed organic compounds.
Determination of levoglucosan and dehydroabietic acid in urban aerosol
Coufalík, Pavel ; Čmelík, Richard ; Křůmal, Kamil ; Čapka, Lukáš ; Mikuška, Pavel
The analytical method involving aerosol sampling into water, quantitative pre-concentration of levoglucosan and dehydroabietic acid, and their determination by LC-MS was developed in this work.
Determination of inorganic anions in air by modernized aerosol sampler
Čapka, Lukáš ; Mikuška, Pavel ; Večeřa, Zbyněk
Combination of original ACTJU sampler with water-based condensation growth unit (CGU) located upstream of the ACTJU allows quantitative sampling of aerosol particles down to a few nm in diameter. The ACTJU effluent is permanently sucked out from the sampler and online analysed for particulate water-soluble species by ion chromatography.
Aerosol size distribution of PAH in urban atmosphere
Bendl, Jan ; Hovorka, Jan (advisor) ; Mikuška, Pavel (referee)
The aim of the study was to determine the 13 health risk PAHs (phenanthrene, anthracene, fluoranthene, pyrene, benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene, benzo(g,h,i)perylene, ideno(1,2,3-cd)perylene and coronene; c-PAU highlighted) in the fractions of size-segregated aerosol of the urban air in Ostrava (2012, 2014) and Mlada Boleslav (2013) at low temperatures in winter, and to verify experimentally a sampling artifact, i.e., to quantify PAHs in the gas phase. For the particle size separation high-volume cascade impactor Hi-Vol BGI 900 was used. PAHs were determined by HPLC-FLD/PDA. In Ostrava in 2012 during the winter inversion (up to -25 řC), mean concentration of 13 PAHs in aerosol was 432 ng.m-3 ; in fraction 10 - 1 µm 119 ng.m-3 , 1 - 0,5 µm 185 ng.m-3 , 0,5 - 0,17 µm 91 ng.m-3 , in fraction < 0,17 µm 37 ng.m-3 and in the gaseous phase of min. 40 ng.m-3 . In the most unfavorable day 2. 2. 2012 (-18.6 ř C) reached the 24-hour average of 13 PAHs 890 ng.m-3 , the sum of carcinogenic PAHs 237 ng.m-3 and benzo(a)pyrene 32.3 ng.m-3 . An irregular and different decline of PAHs concentrations after inversion was measured: in fraction 1 - 10 µm 2.8 times, 0.5 - 1 µm 4.2 times, 0.5 - 0.17 µm 4 times and for <0.17 µm up to 7.6 times....

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