National Repository of Grey Literature 7 records found  Search took 0.00 seconds. 
Determination of stability constants of antamanide complexes with sodium and potassium ions by affinity capillary electrophoresis
Pangavhane, Sachin ; Böhm, S. ; Makrlík, E. ; Ruzza, P. ; Kašička, Václav
Affinity capillary electrophoresis (ACE) was applied for quantitative investigation of the interactions between important biomolecule, cyclic decapeptide antamanide (AA), cycl[-Val(1)-Pro(2)-Pro(3)-Ala(4)-Phe(5)-Phe(6)-Pro(7)-Pro(8)-Phe(9)-Phe(10)-], and sodium and potassium cations in methanol. Apparent stability constants of AA complexes with these alkali metal ions were determined from the dependence of effective mobility of AA on the concentration of these ions in the background electrolyte by the non-linear regression analysis. The AA complexes with Na+ and K+ ions were found to be relatively weak, with the apparent stability constants 343 L/mol and 35 L/mol, respectively.
Determination of stability constants of [Gly6]-antamanide complexes with small cations by affinity capillary electrophoresis
Pangavhane, Sachin ; Makrlík, E. ; Ruzza, P. ; Kašička, Václav
Affinity capillary electrophoresis (ACE) was employed for the determination of apparent stability constants Kst of complexes of cyclic peptide [Gly6]-antamanide ([Gly6]-AA) with alkali and alkali-earth metal ions: Li+, Na+, K+, Rb+, Cs+, NH4+, and Ca2+. Kst was determined from the dependence of effective mobility of [Gly6]-AA on the concentration of these ions in the background electrolyte using the non-linear regression analysis. Kst were found to be in the range 13.3 – 26.3.
Investigation of calix[4]arene-bis(t-octylbenzo-18-crown-6) complex with cesium cation by affinity capillary electrophoresis
Štěpánová, Sille ; Moyer, B. ; Makrlík, E. ; Kašička, Václav
Affinity capillary electrophoresis (ACE) was employed for investigation of non-covalent interactions of calix[4]arene-bis(t-octylbenzo-18-crown-6) with Cs+ ion in methanol. The apparent binding constant of the calix[4]arene-bis(t-octylbenzo-18-crown-6)-Cs+ complex was determined from the dependence of the effective electrophoretic mobilities of the calix[4]arene-bis(t-octylbenzo-18-crown-6) on the concentration of cesium ion in the backround electrolyte using a non-linear regression analysis. The logarithmic form of the conditional apparent binding constant of calix[4]arene-bis(t-octylbenzo-18-crown-6)-Cs+ complex was found to be equal to 1.61.
Application of affinity capillary electrophoresis for quantitative characterization of hexaarylbenzene-based receptor binding with alkali metal ions in methanol
Ehala, Sille ; Makrlík, E. ; Toman, Petr ; Kašička, Václav
Affinity capillary electrophoresis was found to be suitable and effective tool for the quantitative evaluation of strong as well as weak interactions between hexaarylbenzene-based receptor and several alkali metal ions in methanol. The selectivity of the above receptor expressed by binding constants towards alkali metal ions was found to increase in the order of Na+ < Li+ < K+ < Cs+ < Rb+.
Capillary electrophoresis employed for quantitative characterization of peptide interactions with small ions and cyclodextrins
Kašička, Václav ; Ehala, Sille ; Šolínová, Veronika ; Schimperková, Tereza ; Sázelová, Petra ; Koval, Dušan ; Makrlík, E.
Capillary affinity electrophoresis has been applied to investigate quantitatively two types of non-covalent peptide interactions: i) complexes of pseudopeptide valinomycin with ammonium ion and alkali metal ions, Li+, Na+, K+, Rb+ and Cs+, in methanol, and ii) complexes of enantiomers of dipeptide beta alanyl-D,L-tyrosine and its derivatives with chiral selector, 2-hydroxypropyl-beta-cyclodextrin, in water.
Characterization of valinomycin binding with alkali metal ions by capillary affinity electrophoresis and density functional theory
Ehala, Sille ; Dybal, Jiří ; Makrlík, E. ; Kašička, Václav
Capillary affinity electrophoresis (CAE) and quantum mechanical density functional theory (DFT) have been applied to the study of non-covalent binding of valinomycin (Val), an antibiotic dodecadepsipeptide ionophore, with alkali metal ions, Li+, Na+, K+, Rb+ and Cs+, in methanol. The binding constants (Kb) of Val-alkali metal ion complexes were evaluated by CAE. The most probable structures of the Val-alkali metal ion complexes were proposed by DFT.
Studium interakcí amonných iontů a iontů alkalických kovů s valinomycinem kapilární elektroforézou
Ehala, Sille ; Kašička, Václav ; Makrlík, E.
Interactions of NH4+, K+, Na+ and Li+ cations with valinomycin in methanol were investigated. Binding constants of complexes of the above cations with valinomycin were obtained.