National Repository of Grey Literature 12 records found  1 - 10next  jump to record: Search took 0.00 seconds. 
The woman at immunological risk and her infant
Mocková, Alice ; Ulčová-Gallová, Zdeňka (advisor) ; Madar, Jindřich (referee) ; Šedivá, Anna (referee)
Women in childbearing age are often affected by autoimmune diseases (AD) associated with the presence of antiphospholipid antibodies (aPL) that may influence further develop-ment of their children. The primary objective of our prospective study was to determine the presence of the following aPL: anti β2 glycoprotein I (anti-β2GPI), anticardiolipin (aCL), antiphosphatidylserine, antiphosphatidylinositol, antihospha- tidylethanolamine, antiphosphatidylglycerol, antiphosphatidic acid, antiannexin V in mothers with defined AD and their children after birth, at 6 and 12 months of life, and to compare the incidence of aPL with a control group. A secondary objective of the study was a 2-year follow-up of children born to aPL negative and aPL positive mothers with AD in order to detect the possible impact of maternal AD on the health of the offspring. In children, we analysed anthropometric data, blood cell count, cerebral and abdominal ultrasound examination, transient evoked otoacoustic emission test (TEOAE), electrocardiograph (ECG), the presence and kinetics of aPL. At the age of 2 years the Bayley Scales of Infant Development (BSID-II) were used for children's assessment of motor, language and cognitive development. 31 mothers from the total examined 82 aPL positive women with AD delivered 34 neonates...
Observation of human sperm cell immunogenic properties
Cibulka, Jan ; Ulčová-Gallová, Zdeňka (advisor) ; Fingerová, Helena (referee) ; Madar, Jindřich (referee) ; Šíma, Petr (referee)
The issue of human infertility is becoming increasingly pressing, especially in so- called industrially developed countries. Formally, we can share the infertility attributes between the male and the female partner (as a male and female infertility factor), but the problem finally affects the whole parental couple. Perhaps, due to the complexity of the female reproductive tract, it is estimated that over 40% of the reproductive failure is hidden in one or more of its parts. The "strong point" of the male factor is derived from the pathological sperm count. For the remaining 15 to 20% of causes, whether morphological or functional, cannot be to find either clearly or not at all. We talk about the otherwise unexplained infertility, that can be caused in up to 30% immunologically. Due to broad concept of immunology of reproduction, we have chosen literally as a probe into this issue in all respects unique spermatic cell that represents not only a carrier of genetic information, but also a set of antigenic structures. This cell seems to be perfect for this task, because of its availability to most basic laboratory techniques. We will try to familiarize ourselves with some of the obstacles that may prematurely end its mission, especially with the antibodies against its antigenic structures (ASA -...
Preeclamsia and selected immunogenetic factors
Hradecký, Libor ; Ulčová-Gallová, Zdeňka (advisor) ; Madar, Jindřich (referee) ; Oborná, Ivana (referee)
Preeclampsia and selected immunogenetic factors Our objective was to evaluate plasma levels of the eight most common antiphospholipid antibodies (antiphosphatidylserine, antiphosphatidylethanolamine, antiphosphatidylinositol, antiphosphatidylglycerol, antiphosphatidic acid, antiannexin V, anticardiolipin and anti 2-glycoprotein I antibodies) by ELISA method and selected inherited thrombophilia (F V- Leiden mutation, FII mutation G20210A, C677T and A1298C variants of the gene for methylene tetrahydrofolate reductase-MTHFR) by DNA analysis of peripheral blood lymphocytes using the real-time PCR in fifty-five women with preeclampsia in the period immediately before urgent termination of pregnancy. Fifty-five healthy women without preeclampsia was considered as a controll group. Entered data were examined using a non-parametric Wilcoxon's test, univariate analysis were perfomed using the Fisher's exact test and statistical dependence between variables was assessed using Spearman's rank correlation coefficient. We demonstrated that women with preeclampsia had significantly higher levels of anticardiolipin antibodies in the isotope IgG (p <0.01) and IgM (p <0.01), elevated levels of antiphosphatidylserine antibodies in the isotope IgG(p <0.01) and antiethanolamine antibodies in the isotope IgM (p <0.01) when...
The role of immune system factors in IVF/ET success
Růžičková, Žaneta ; Pěknicová, Jana (referee) ; Madar, Jindřich (advisor)
5 Abstract The aim of this study was to evaluate the presence of receptors (CD16, CD161, NKG2D, KIR2DL4 and CD85j) on the surface of immunocompetent cells and whether their presence is involved in the in vitro fertilization (IVF) and following embryotransfer (ET) success. We observed significant difference in the age of successful versus unsuccessful patients, which is confirmed by former studies on age-dependence of IVF success. The result from cytotoxic assay confirmed, that neither the stimulation protocol, nor the PBMC source have any effect on effector function. Afterwards, we performed a large-scale analysis of surface markers, starting with healthy donors, compared to patients. Both groups of patients have lower counts of HLA-G binding KIR2DL4 or CD85j receptors bearing cells. Next we observed the expression of these two receptors on various subpopulations of cells (CD56+dim , CD56+bright , T helpers and cytotoxic T cells) and their counts. Here we observed differences in CD56 surface expression on CD56+ bright NK cells, the levels of CD56+dim NK cells in PBMC (CD16 expression on this population), levels of CD56+dim Next step was to compare the levels of cell populations between the successful and unsuccessful patients. We observed an increased surface expression of KIR2DL4 on lymphocytary PBMC of...
Thyroid gland disorder and female reproductive dysfuntion
Melicharová, Lucie ; Madar, Jindřich (advisor)
My thesis I called Thyroid disease and reproductive disorders women. It is a subject standing at the interface between gynecology, immunology and endocrinology. Reproductive interested me during the compulsory optional courses: Immunology female reproduction and reproductive immunology. His seminary work I was writing to topic antibodies against sperm. Now I have decided to address the topic of the thyroid gland. A reason - in the future I like to pay in gynecology and obstetrics, with which the topic is very closely related. In most developed countries, it is still a current issue declining birth rate. One reasons for the shift of family planning in higher age categories, then it is lower number of children in families, but also is a big contributor of reduced reproductive ability pairs. According to statistics of infertility in economically developed countries is increasing and at present currently affects 10-15% of couples, ie every 7 to 10 pair (Ventruba, 2001). The causes of infertility is involved in 30-35% only man and woman only 35-40%. A combination of factors of both partners is present in 25% of infertile couples, and it is necessary to note that in both men and women can occur more causes simultaneously. Only 5% of couples to seek medical help to detect apparent cause sterility (Ventruba, 2001)....

National Repository of Grey Literature : 12 records found   1 - 10next  jump to record:
See also: similar author names
1 Madar, J.
1 Maďar, Jaroslav
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