National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Women in ICT professions and ICT sector in the Czech Republic
Kristová, Markéta ; Šiklová, Jiřina (advisor) ; Dudová, Radka (referee) ; Riegel, Karel (referee)
This thesis analyses the low representation of women in the ICT professions as well as their inadequate representation in the ICT companies in the Czech Republic. Given it is the very first study of this kind in the Czech Republic, it also provides comprehensive catalogue of relevant foreign theoretical studies and hypothesis, which examine the issue of gender gap in the ICT. This thesis also deals with social and historical factors that - as approved by numerous empirical studies - have negative impact on the way girls and women relate to information and communication technologies, resulting in a low representation of women in the ICT professions. The above factors are applied to the context of the Czech society, using academic studies and other relevant sources in the field of equal opportunities, including qualitative interviews, which I conducted with ICT teachers and lecturers as well as women and men working in the ICT field. Not last, the thesis builds upon a qualitative empirical probe, which aims to introduce more detailed information about the experiences of women holding ICT positions in the Czech Republic. This particular research failed to prove the presumption that the work environment in the ICT companies in the Czech Republic is not sufficiently friendly or obliging towards women. Moreover,...
Identification of selected genes in lactic acid bacteria
Kristová, Mária ; Vojtíšková, Marie (referee) ; Španová, Alena (advisor)
Lactic acid bacteria are natural habitants of human gastrointectinal tract. Among the most important are bacteria of genus Lactobacillus and genus Bifidobacterium that contain a lot of probiotic species. Probiotic species are used as food supplements. This work was focused on DNA separation from crude cell lysates of 4 food supplements using magnetic carrier P(HEMA-co-GMA) covered by carboxyl groups. DNA was reversible adsorbed to the carriers in the presence of PEG 6000 (16%) and NaCl (2 M) (final concentrations) and eluted into TE buffer. Lysis of cells from food supplements was performed by lysozyme, SDS and proteinase K. The amount of lysozyme was optimalized. Concentration of separated DNA was measured by spectrophotometric method. The amount of isolated DNA was suitable for PCR. Isolated DNA was used for PCR with universal primers, PCR specific for genus Lactobacillus and genus Bifidobacterium and for 9 different species-specific PCRs: Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus fermentum, Lactobacillus casei/paracasei, Streptococcus thermophilus, Bifidobacterium longum, Bifidobacterium bifidum and Bifidobacterium infantis. Amplicons were detected by agarose gel electrophoresis (1,8%). It was shown that DNA amplification methods are quick and precise for identification of studied species. The results of bacteria identification were compared with data provided by the manufacturer. In all food supplements, bacteria of genus Lactobacillus and Bifidobacterium were detected. However, only some species provided by manufacturer were identified by PCR in each tablet.

See also: similar author names
1 Kristová, M.
1 Kristová, Markéta
2 Kristová, Michaela
1 Kristová, Mária
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