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Comparison of various amination methods of polycaprolactone concerning their effectivity in tissue engineering
Kováč, Ján ; Lehocký, Marián (oponent) ; Zajíčková, Lenka (vedoucí práce)
This diploma thesis dealt with the comparison of different methods of amination of polycaprolcatone in terms of their effectiveness for tissue engineering. A polycaprolactone membrane was prepared by an electrospinning method, which was subsequently modified by three different amination methods. Selected types of amination were plasma polymerization with cyclopropylamine monomer, hybrid modification using plasma and N-allylmethylamine monomer, and chemical amination using aminolysis with diaminohexane. Surface amines were subsequently characterized by electron scanning microscopy (SEM), X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-FTIR) and contact angle measurement. A cell culture designated A375 (Human malignant melanoma cell lines A375® CRL-1619®) was cultured on the thus modified membranes, which was analyzed by optical microscopy, and a proliferation assay was performed by determining the relative amount of ATP. Based on the experimental results, we can confirm the success for all types of amination. In terms of efficiency for tissue engineering, the amination method by plasma polymerization with the monomer cyclopropylamine has the most satisfactory results.
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SW pro přístupový panel data koncentrátoru
Kováč, Jakub ; Franek, Lešek (oponent) ; Šťastný, Ladislav (vedoucí práce)
Cieľom tejto práce je tvorba SW pre prístupový panel data koncentrátora. Práca rozoberá problematiku sériovej komunikácie medzi HMI a data koncentrátorom. Pre zariadenie je navrhnutý komunikačný protokol a grafické rozhranie aplikácie. Následne je predvedený návrh algoritmu riadiacej aplikácie. Algoritmus je navrhnutý s ohľadom na batériové napájanie data koncentrátora použitím low-power operačných režimov.
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Surface treatment of biocompatible materials
Kováč, Ján ; Kučera, František (oponent) ; Krčma, František (vedoucí práce)
This bachelor thesis deals with the study of the influence of plasma discharge on changes in the surface properties of the biomaterial. Surface treatements were performed using three types of plasma discharge: gliding arc, dielectric barrier discharge (DBD) and microwave surface wave discharge. Various gaseous mixtures in plasma discharges and their effect on the surface treatment of the biomaterial were investigated. The selected biomaterial was aliphatic biopolymer polycaprolactone (PCL). It is a suitable surface treatment material, thanks to its biodegradability and easy processability. The PCL supplied by Sigma-Aldrich was in the form of pellets, witch had been converted to foil. The film samples were subsequentely exposed to plasma discharges. To characterize the surface properties of the polymer, surface energy was measured using a sissle drop method, a See System from Advex Instrumnets was used to measure. Based on experimental results, we can confirm that the surface has been modified. However, the plasma discharge modifications have led to hydrophilic and not hydrophobic properties of the biomaterial, with the best results in terms of hydrophilicity being achieved by microwave discharge with surface wave. Due to the negative results of the experiment, bactericidal effects were not investigated.
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Asymptotic Control of Portfolio for several assets
Kováč, Jakub ; Dostál, Petr (vedoucí práce) ; Justová, Iva (oponent)
We consider an investor who invests in a stock and money market and whose goal is to maximize the market value of her portfolio in the very long run. The goal of the thesis is to find an optimal trading strategy for the investor. The stocks' market values are simulated by multidimensional Brownian motion. The possibility to buy and sell stocks introduces a new dimension to the dynamics of the problem. By using the Itoo calculus we derive the basic properties of the continous model. Considering the continous model difficulties with finding the optimal trading strategy, we aproximate the continous model by a dsicrete model. In the end, the thesis presents hints to use the Howard algorithm in the discrete case. The main contribution of the thesis is the introduction and proof of the Howard algorithm which can be used as a tool to find the optimal trading strategy in the discrete model.
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Comparison of various amination methods of polycaprolactone concerning their effectivity in tissue engineering
Kováč, Ján ; Lehocký, Marián (oponent) ; Zajíčková, Lenka (vedoucí práce)
This diploma thesis dealt with the comparison of different methods of amination of polycaprolcatone in terms of their effectiveness for tissue engineering. A polycaprolactone membrane was prepared by an electrospinning method, which was subsequently modified by three different amination methods. Selected types of amination were plasma polymerization with cyclopropylamine monomer, hybrid modification using plasma and N-allylmethylamine monomer, and chemical amination using aminolysis with diaminohexane. Surface amines were subsequently characterized by electron scanning microscopy (SEM), X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-FTIR) and contact angle measurement. A cell culture designated A375 (Human malignant melanoma cell lines A375® CRL-1619®) was cultured on the thus modified membranes, which was analyzed by optical microscopy, and a proliferation assay was performed by determining the relative amount of ATP. Based on the experimental results, we can confirm the success for all types of amination. In terms of efficiency for tissue engineering, the amination method by plasma polymerization with the monomer cyclopropylamine has the most satisfactory results.
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Doplnění lesnického skanzenu v Čiernom Balogu o objekt vodní píly
Kováč, Jozef
Do své úvodní části tato práce subsumovala historické prameny vodních pil a jejich konstrukci, podle nichž je vypracována dokumentace pro plánovaný stavbu pily a její součásti. Tato práce navrhuje různé typy pohonu pily z hlediska konstrukce, a na základě porovnání přínosů a nedostatků doporučuje optimální typ tohoto pohonu. Součástí práce je kromě důležitých výpočtů i výkresová dokumentace, ve které jsou rozkresleny objekty pilnice a jejich součásti. Takto rozpracovaný návrh bude sloužit jako podklad pro realizaci stavby vodní pily jakožto technické památky, která bude návštěvníkům skanzenu přibližovat způsob, jakým způsobem zpracovávalo dřevo v minulosti.
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