|
|
ISOLATION OF TRANSGENIC PLANTS NICOTIANA TABACUM AND SILENE VULGARIS
Kováčová, Viera ; Doškař, Jiří (oponent) ; Vyskot,, Boris (vedoucí práce)
This project is focused on transformation of Silene vulgaris mediated by Agrobacterium tumefaciens and A. rhizogenes. S. vulgaris is a good model plant to study gynodioecy, an evolutionary step from bisexuality to dioecy. Gynodioecious plants form in some individuals bisexual flowers, while the others possess only female flowers. The aim of this research is do develop a technique to introduce foreign genes into this plant to study its developmental consequences. Using A. rhizogenes we successfuly prepared hairy root cultures, which unfortunately do not form shoot regenerants. We have prepared a protocol to induce plant regenerants from S. vulgaris leaf fragments. The first results do not confirm that A. tumefaciens infected plant regenerants harbor reporter transgenes. We used Nicotiana tabacum as a positive control.
|
|
|
Agrobacterium tumefaciens application
Kováčová, Viera ; Burdychová, Radka (oponent) ; Babák, Libor (vedoucí práce)
This project focus on biotechnologic applications of bacterium named Agrobacterium tumefaciens, which is capable to mediate genetic transformation not only for plants. Main points of project are divided between description of basic natures and cytology of A. tumafaciens following by overview of usage A. tumefaciens in methods of modifications of diffrent cells. Target of paper about biotechnologic use of A. tumefaciens is confrontation of effectivity of this method of modification with else, summary of positiv benefits of its using for future development of human population and introduction of fundamental transformation methods of modification most important crop plants. Final part of project is brief summation of basic statement from statistics and legislature of genetic modified crops.
|
|
|
ISOLATION OF TRANSGENIC PLANTS NICOTIANA TABACUM AND SILENE VULGARIS
Kováčová, Viera ; Doškař, Jiří (oponent) ; Vyskot,, Boris (vedoucí práce)
This project is focused on transformation of Silene vulgaris mediated by Agrobacterium tumefaciens and A. rhizogenes. S. vulgaris is a good model plant to study gynodioecy, an evolutionary step from bisexuality to dioecy. Gynodioecious plants form in some individuals bisexual flowers, while the others possess only female flowers. The aim of this research is do develop a technique to introduce foreign genes into this plant to study its developmental consequences. Using A. rhizogenes we successfuly prepared hairy root cultures, which unfortunately do not form shoot regenerants. We have prepared a protocol to induce plant regenerants from S. vulgaris leaf fragments. The first results do not confirm that A. tumefaciens infected plant regenerants harbor reporter transgenes. We used Nicotiana tabacum as a positive control.
|
|
|
Agrobacterium tumefaciens application
Kováčová, Viera ; Burdychová, Radka (oponent) ; Babák, Libor (vedoucí práce)
This project focus on biotechnologic applications of bacterium named Agrobacterium tumefaciens, which is capable to mediate genetic transformation not only for plants. Main points of project are divided between description of basic natures and cytology of A. tumafaciens following by overview of usage A. tumefaciens in methods of modifications of diffrent cells. Target of paper about biotechnologic use of A. tumefaciens is confrontation of effectivity of this method of modification with else, summary of positiv benefits of its using for future development of human population and introduction of fundamental transformation methods of modification most important crop plants. Final part of project is brief summation of basic statement from statistics and legislature of genetic modified crops.
|
host ::
RSS.