National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Study of the effect of cyclin-dependent kinase inhibitors on the expression of selected AKR and CBR enzymes in human cell lines.
Kouklíková, Etela ; Novotná, Eva (advisor) ; Wsól, Vladimír (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Etela Kouklíková Supervisor: RNDr. Eva Novotná, Ph.D. Title of diploma thesis: Study of the effect of cyclin-dependent kinase inhibitors on the expression of selected AKR and CBR enzymes in human cell lines Cyclin-dependent kinase inhibitors (CDKi) are considered as a suitable treatment especially in patients with wrong prognosis or advanced stage of cancer. It has only recently been discovered that CDKi are able to influence the activity of some enzymes from aldo-keto reductase (AKR) and short-chain dehydrogenase/reductase (SDR) superfamilies. AKR and SDR enzymes belong to a group of carbonyl reducing enzymes that are involved in the metabolism of endobiotics and xenobiotics. An important group of drugs that are metabolized by these enzymes to less efficient compounds are anthracyclines. The aim of this diploma thesis was to find out whether purvalanol A, roscovitin, dinaciclib, AZD5438 and R547 can affect the expression of the most important anthracycline reductases (AKR1A1, AKR1B10, AKR1C3, AKR7A2 and CBR1) in human HepG2 and HL-60 cell lines. Expression of anthracycline reductases in cells exposed to CDKi was evaluated at mRNA level by RT-qPCR and at protein level by Western blotting. The...
Study of the effect of cyclin-dependent kinase inhibitors on the expression of selected AKR and CBR enzymes in human cell lines.
Kouklíková, Etela ; Novotná, Eva (advisor) ; Wsól, Vladimír (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Etela Kouklíková Supervisor: RNDr. Eva Novotná, Ph.D. Title of diploma thesis: Study of the effect of cyclin-dependent kinase inhibitors on the expression of selected AKR and CBR enzymes in human cell lines Cyclin-dependent kinase inhibitors (CDKi) are considered as a suitable treatment especially in patients with wrong prognosis or advanced stage of cancer. It has only recently been discovered that CDKi are able to influence the activity of some enzymes from aldo-keto reductase (AKR) and short-chain dehydrogenase/reductase (SDR) superfamilies. AKR and SDR enzymes belong to a group of carbonyl reducing enzymes that are involved in the metabolism of endobiotics and xenobiotics. An important group of drugs that are metabolized by these enzymes to less efficient compounds are anthracyclines. The aim of this diploma thesis was to find out whether purvalanol A, roscovitin, dinaciclib, AZD5438 and R547 can affect the expression of the most important anthracycline reductases (AKR1A1, AKR1B10, AKR1C3, AKR7A2 and CBR1) in human HepG2 and HL-60 cell lines. Expression of anthracycline reductases in cells exposed to CDKi was evaluated at mRNA level by RT-qPCR and at protein level by Western blotting. The...
Concentration of nickel in wastewater and its removal using a constructed wetland
KOUKLÍKOVÁ, Etela
This bachelor thesis is focused on the determination of the efficiency of the nickel removal from wastewater using constructed wetlands. During 2015, wastewater from the vegetation wastewater treatment plant in the villageSlavošovice was analyzed. Nickel concentration in wastewater was determined by atomic absorption spectrometry. Samples of wastewater were taken eight times from six different parts of the wetland. The obtained results of the wastewater analysis I evaluated the degree of the nickel removal in the profile of the vegetation wastewater treatment plant. The analysis was conducted using an atomic absorption spectrometer Thermo Scientific iCE 3500. The characteristics of the analytical method were as follows: accuracy (97.64%), precision (3.53%), sensitivity (9 x 10-3 s.l/g), limit of detection (LOD = 0.161 ?g /l), limit of quantification (LOQ = 0.537 ?g/l), linear dynamic range (0,537 - 10 ?g/l) and correlation coefficient (0.9992). The concentration of nickel obtained from samples from the inflow zone differed by several tenths or units of ?g/l compared to the inflow concentration value. The nickel of concentration at the outflow from the system was decreased by units of ?g/l compared to the inflow. The gradual decrease of the concentration of nickel was observed for the samples taken from the profile of the constructed wetland vegetated bed. The obtained results document the sufficient efficiency of the nickel removal from municipal wastewater in Slavošovice constructed wetland. The outflowing water, guided to a nearby stream, contains lower concentrations of nickel in comparison with the raw wastewater. With respect to aquatic organisms, it is very important to reduce the concentration of nickel in treated wastewater. Actually, the nickel toxicity for aquatic organisms is much higher than for humans.

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3 KOUKLÍKOVÁ, Etela
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