National Repository of Grey Literature 24 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
The role of energy metabolism in cardioprotection induced by the adaptation to chronic hypoxia
Kolář, David ; Žurmanová, Jitka (advisor) ; Adamcová, Michaela (referee) ; Bardová, Kristina (referee)
Cardiac energy metabolism is the one of the most complex system in the body. To sustain life, but also to respond quickly to any sudden changes (e.g. running, emotional stress), the heart has developed a unique ability and has become a metabolic "omnivore". At physiological conditions, long chain fatty acids (LCFAs) present the major energetic source for the adult myocardium. However, the cardiac energy metabolism may be compromised during pathophysiological states. One of the most dangerous is, undoubtedly, ischaemia-reperfusion injury with its acute form, myocardial infarction. However, the adaptation to chronic hypoxia has been known for decades for its cardioprotective effect against I/R. Changes of cardiac energy metabolism induced by the adaptation have not been fully explored and the system conceals still too many secrets. This thesis has aimed to determine how adaptation to chronic hypoxia affects the cardiac metabolism of the rat LVs in the following set-ups: 1. The effect of chronic normobaric hypoxia (CNH; 3 weeks, 5500m) during a brief I/R protocol in vitro on the protein kinase B/hexokinase (Akt/HK) pathway, including the expression and phosphorylation of Akt, the expression and localization of HK, the expression of mitochondrial creatine kinase (mtCKS), and the level of Bcl-2 family...
His bundle in phylogenesis, ontogenesis and pathology
Tremerová, Aneta ; Gregorovičová, Martina (advisor) ; Kolář, David (referee)
His bundle is a part of the cardiac conduction system of mammals, birds and crocodilians. His bundle is important for high cardiac output in endothermic vertebrates. His bundle is a connection between atria and ventricles in fibrous atrioventricular isolation and it serves as an electrical conduction pathway via bundle branches to ventricular apex, where ventricular contraction begins. Defect in cardiac conduction system leads to cardiac arrhytmia and can cause sudden cardiac death. Thesis describes development of His bundle in mammals and birds, its phylogenensis and atrioventricular conduction in other vertebrates. Thesis also describes disorders of atrioventricular canal development, fibrous anulus and His bundle such a preexcitation syndromes and congenital heart block. Thesis also depicts history of a discovery of the atrioventricular conduction tissue. Key words: cardiac conduction system, His bundle, atrioventricular canal, anulus fibrosus, ventricular preexcitation, perinatal AVRT, WPW syndrome, congenital heart block
Adenosine signaling: the role in neuroprotection and neurodegeneration
Hrušovská, Kateřina ; Novotný, Jiří (advisor) ; Kolář, David (referee)
The aim of this bachelor thesis is to describe basic and the most important mechanisms of adenosine signaling, especially in the central nervous system, where the purine nucleoside adenosine plays important role like significant neuromodulator. Strong release of adenosine to extracellular space may occur under some pathological conditions. Adenosine works throught his four receptors, which have very diverse functions. Some effects are neuroprotective - these are predominantly mediated throught the inhibitory A1 receptor, which can reduce neurotoxicity, others may also induce neurodegeneration, mainly due to increased activation of A2A receptors. This signaling system can be diversely modulated, for example by inhibition of enzymes, which can provide adenosine formation or degradation, blocking its transporters, by agonists or adenosine antagonists, or by inhibition of second messengers and various protein kinases by which adenosine affects cellular processes. Interactions of adenosine receptors with other types of receptors in the brain are also important. Adenosine and adenosine receptors can participate in neurodegenerative processes. A detailed understanding of the specific effects of adenosine can bring great progress in the treatment of neurodegenerative diseases. At present, intensive...
Peroxisomes and their role in energetic metabolism of the heart
Čejková, Denisa ; Kolář, David (advisor) ; Horníková, Daniela (referee)
Peroxisomes are small eucaryotic organelles, mainly known for their ability to create and break down hydrogen peroxide. However their enzymes play other significant roles, they participate in beta-oxidation of fatty acids with very long or branched chains, through alpha-oxidation they enable to process molecules that cannot go through beta- oxidation cycle, they also participate in early steps of synthesis of ether-lipid or bile acides. Above mentioned aspects make peroxisomes potentionally interesting for their possible influence on heart muscle metabolism, that is dependent on oxidative degradation of fatty acids, although not very much is known about this issue. The thesis focuses on biogenesis and function of peroxisomes, but also on their possible role in heart muscle metabolism.
The use of JavaScript MVC frameworks for creating web applications
KOLÁŘ, David
The objective of the bachelor thesis will be develop and describe issues related to JavaScritp MVC frameworks. How to simplify and streamline the work of the code when creating web applications in terms of client-side scripting. I will focus to compare the busiest frameworks, their advantages, disadvantages, when and why it pays to use. I will include in my selection AngularJS, React, Knockout and mention the other frameworks. The practical part will consist of examples particular frameworks while solving real problems. For this purpose, I will create a Web application, which will include an overview of their properties, and comparing the aforementioned examples.
Criminal Liability within Pharmacy
Kolář, David ; Sovová, Olga (advisor) ; Císařová, Dagmar (referee)
The main purpose of this thesis is to analyse the possible application of criminal liability instruments within pharmacy. Chapter 1 introduces the general aspects and processes within pharmacy, general terms, and short preview to the life cycle of innovative medicinal products. Chapter 2 contains the general definition and requirements of liability. There is also a short comparison of additional types of liability (such as civil, employee's, administrative and disciplinary) that can be applicable within pharmacy. The purpose of this comparison is to specify differences between individual liabilities and to prove that they can be applied conjointly. As an example of such a use I have described the "heparin murder" case. Chapter 3 is dedicated to criminal liability, with the explanation of general terms, especially crime, unlawfulness, offender, or grounds of justification. Chapter 4 focuses on the criminal liability of natural persons. After the general introduction in the first part, a list of potential crimes committable within pharmacy is given. Chapter 5 summarizes the criminal liability of legal persons. The first part focuses on the history of implementation in the Czech Republic, general terms, and definition of a legal person as a subject of criminal liability. The second part contains...
Trafficking of purinergic P2X receptor in eukaryotic cell
Pražák, Šimon ; Tvrdoňová, Vendula (advisor) ; Kolář, David (referee)
Purinergic receptors are membrane ion channels activated by extracellular ATP. In vertebrates, seven genes encoding P2X subunits was found. These subunits are designated as P2X1 - 7. Every P2X receptor subunit consists of two transmembrane domains, extracellular domain and intracellular N- and C- termini. P2X receptors fold to homo- or heterotrimers. P2X receptors have a wide distribution in the organism, functional receptors are found in neurons, glial cells, muscle cells and also in nonexcitable tissues as epithelial, endothelial, and in hemopoietic tissue. Purinergic signalling plays an important role in pain transmission, CNS injury and immune processes. P2X receptors are synthesized on the rough endoplasmic reticulum and are transported to the plasma membrane after post-translational modifications in the Golgi apparatus. The distribution and transport of P2X receptors is subunit specific and dependent on the cell type in which they are expressed. P2X receptors can be divided into three groups according to the way they are moved in the cell, which differ in transport speed, plasma membrane accumulation rate and rate of internalization.
The role of microRNA in hypoxic pulmonary hypertension
Tichý, Václav ; Hampl, Václav (advisor) ; Kolář, David (referee)
Pulmonary Arterial hypertension (PAH) is a devastating progressive disease that significantly decreases quality of life and has the average survival rate of only few years. One of the significant initiators of PAH is chronic hypoxia. After more than six decades of research that was initiated in 1946 by von Euler and Liljestrand, a new group of potential regulators of this pathology was discovered, that became heavily studied in the last five years. They are highly conserved molecules belonging to non-coding RNA. These 19-23 nucleotides long microRNA (miRNA) act as negative regulators of expression on various proteins. Many of them regulate traditional signalling pathways of hypoxic PAH (HIF-1, BMPR2) and miRNA is in turn regulated by other signalizations. Together, that creates an interconnected network of direct and indirect interactions and feedback loops, that we need to study in order to understand hypoxic PAH. This thesis summarizes findings about important miRNA molecules from the last few years and elucidates part of these regulatory mechanisms on several miRNA molecules (miR-17-92, miR-21, miR-210, miR-204 a miR143/145).
Beta-adrenergic receptors and their desensitization
Biriczová, Lilla ; Novotný, Jiří (advisor) ; Kolář, David (referee)
β-Adrenergic receptors (β-ARs) are G-protein-coupled receptors (GPCR), widely present in the animal organism and mediate catecholamine pathways leading to diverse physiological responses. The family of β-ARs consists of β1-AR, β2-AR and β3-AR, which are distinguished by their affinity to adrenaline and noradrenaline. A typical model of β-AR signalling includes binding of the ligand, G-protein coupling, activation of adenylyl cyclase (AC) resulting in production of the second messenger cAMP and activation of protein kinase A (PKA) that phosphorylates downstream proteins leading to physiological responses. Beacause excessive catecholamine signalling can cause undesirable consequences, a mechanism has evolved, which attenuates the function of β-ARs in spite of further stimulation, so called desensitization. The classic course of desensitization consists of characteristic steps including phosphorylation of the receptor, β-arrestin attachement and uncoupling of the G-protein from β-AR. Restoration of the signalling ability is allowed through resensitization of β-AR when the receptor is sequestrated and dephosphorylated. Given that β-ARs are structurally and genetically different, it is reasonable to consider that each step of the desenstization process may happen differently among the different subtypes...

National Repository of Grey Literature : 24 records found   1 - 10nextend  jump to record:
See also: similar author names
11 KOLÁŘ, David
5 Kolář, Daniel
1 Kolář, Dominik
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