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Interactions of alkaloids with transition metals I.
Rzepecká, Radka ; Macáková, Kateřina (advisor) ; Karlíčková, Jana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany Candidate: Radka Rzepecká Supervisor: Ing. Kateřina Macáková, Ph.D. Title of Thesis: Interactions of alkaloids with transition metals I. Copper is one of the essential trace elements that are necessary for the proper functioning of the organism. Copper is a significant component of many enzymes that affect various metabolic processes in the human body. It is important that the level of copper in the body is regulated because its deficit or excess lead to a variety of pathological conditions. Alkaloids are secondary plant metabolites that are distinguished by numerous biological activities. In this thesis the copper-chelating and copper-reducing activity of eleven isoquinoline alkaloids was measured: boldine, isocorydine, (+)-bulbocapnine, (+)-corydine, glaucine, (-)-sinoacutine, (-)-californidine, (-)-escholtzine, platycerine, (-)-fumaricine, and (+)- parfumine. Alkaloid activity was measured at four (patho) physiological pH values by a verified spectrophotometric method using two indicators: bathocuproinedisulfonic acid disodium salt and hematoxylin. Based on the results, structure-effect relationships were derived. The results show that none of the tested substances was able to chelate copper ions. In...
Natural compounds applicable for the treatment of metabolic syndrome
Hradecká, Michaela ; Karlíčková, Jana (advisor) ; Kašparová, Marie (referee)
Hradecká M.: Natural compounds applicable for the treatment of metabolic syndrome, Diploma thesis 2018/2019, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany, pp. 60. Occurence of metabolic syndrome is increasing worldwide in children and adults. The use of natural compounds is one of possibilities in the prevention and treatment of metabolic syndrome. These compounds may act complexly or affect individual risk factors associated with metabolic syndrome. Selected plants with these effects are incorporated into my diploma thesis (for example Allium sativum, Crataegus laevigata, Hibiscus sabdariffa, Persea americana, Rosmarinus officinalis, Silybum marianum and Vaccinium myrtillus). The alga Undaria pinnatifida and fungus Pleurotus sajor-caju are also mentioned. It is necessary to carry out other clinical studies, where the positive effect of mentioned constituents will be confirmed, which could be added to the list of phytopharmaceuticals used to prevent, slow down or treat the metabolic syndrome in the future. Keywords: natural compounds, metabolic syndrome
Interactions of tamarixetin and isorhamnetin with copper
Lomozová, Zuzana ; Karlíčková, Jana (advisor) ; Chocholouš, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany Candidate: Zuzana Lomozová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Interactions of tamarixetin and isorhamnetin with copper Keywords: tamarixetin, isorhamnetin, chelation, reduction, copper, bathocuproine, hematoxylin Copper is a biogenic trace element important for proper function of human organism. It is an essential part of several enzymes and is involved in metabolic processes in the body. Excess or lack of serum copper can lead to pathological conditions. Copper chelating agents are used to treat copper toxic effects. Flavonoids are polyphenolic substances belonging to secondary metabolites of various plants. They are part of the human diet and have a positive impact on our health. They exhibit antioxidant and anti-inflammatory effects and are able to chelate transient metals, especially iron and copper. Chelation therapy is currently used in Wilson's disease in which copper is overloaded. In the future, the chelating effects of flavonoids could be used to treat neurodegenerative diseases, cardiovascular diseases or cancer. In this diploma thesis, interactions of two flavonoids (namely tamarixetin and isorhamnetin) with copper ions in different buffers were tested....
Environmental risks of anthelmintics
Dojčanská, Nikoleta ; Szotáková, Barbora (advisor) ; Karlíčková, Jana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Nikoleta Dojčanská Supervisor: prof. Ing. Barbora Szotáková, Ph.D Title of diploma thesis: Environmental risks of anthelmintics Anthelmintics are drugs used in human and veterinary medicine for the treatment and prophylaxis of infectious diseases caused by parasites- helminths. They are admittedly beneficial to the treated organisms. However, their overall effect on the ecosystem has not yet been sufficiently explored. Anthelmintics, together with their metabolites, enter the environment in multiple ways. One possibility is directly through urine and excrements of treated livestock or indirectly by leakage of contaminated manure into surface and groundwater. Such pollution has an effect on various animal organisms and plants that have the ability to uptake xenobiotics and remove them through metabolic pathways. The aim of this work was to investigate the effect of antihelmintics ivermectin and fenbendazole on soybean plants (Glycine max). We investigated the concentration of total phenolic compounds spectrophotometrically using the Folin-Ciocalteu method. Using the colorimetric method of aluminum chloride, we found the concentration of flavonoids in soybean plants. The concentration of isoflavons-...
The effect of methylviologen on secondary metabolites production in in vitro culture of Fagopyrum esculentum, variety Bambi
Vlachová, Veronika ; Tůmová, Lenka (advisor) ; Karlíčková, Jana (referee)
10 ABSTRACT The subject of this study was to evaluate the efect of abiotc elicitor on rutn producton in callus and suspension cultures of buckwheat. The cultvar of buckwheat used for this research was Fagopyrum esculentum Moench var. Bambi, cultvated in Murashige and Skoog nutrient medium with the additon of growth regulator 2,4-dichlorfenoxyacetc acid (2,4-D) in concentraton of 1 mg/l. The elicitor used in this study was a soluton of methylviologen, 1 ml of it was added to the cultures in three diferent concentratons: c1 = 100.0 mg/100 ml, c2 = 10.0 mg/100 ml and c3 = 1.0 mg/100 ml. The elicitor was afectng the cultures for 6, 12, 24, 48, 72 or 168 hours. Afer the defned period of tme, cultures were collected, dried out and stored for further analysis of rutn content. To control samples (without elicitor treatment) 1 ml of ethanol 96% was added and they were collected afer 6, 24, 72 or 168 hours. Releasing of rutn into the nutrient medium was also investgated. Rutn content in each sample of cultures and in each sample of nutrient medium was later determined by HPLC. Any signifcant increase in the producton of rutn was not observed in this study. The maximum amount of rutn detected was 0.1 mg/g DW, thus the lowest quantty detectable, and was found in suspension cultures in three cases, afer the additon of...
Comparison of interactions of equol and desmethylangolensin with iron and copper
Němcová, Hana ; Karlíčková, Jana (advisor) ; Matysová, Ludmila (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany Candidate: Hana Němcová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Comparison of interactions of equol and desmethylangolensin with iron and copper Iron and copper are essential trace elements, which are important for our body. Both elements have a significant effect on the correct function of organs and make part of many enzymes. They are able to accept or donate electrons - conversion between oxidized (Fe3+, Cu2+) and reduced (Fe2+, Cu+) forms. If these metals are excess in organism, they are accumulated in the cells and mediate the creation of free radicals, that destroy cell structures. This deficiency is treated with chelators, which facilitate the excretion of metals from the body. Isoflavonoids are polyfenolic substances, which can have antioxidant effects and they are involved in the scavening of free radicals. Isoflavonoids can have also a pro-oxidative effect, because they are able to reduce metal ions. In this study were tested interactions (chelation and reduction) between the metabolites of isoflavonoids (equol and desmethylangolensin) and ions of iron and copper. Both metabolites only weakly chelate metal ions, but they significantly reduce cupric ions. KEYWORDS: Iron,...
Comparison of copper ion chelation by benzoic acids
Gorbunovová, Eva ; Karlíčková, Jana (advisor) ; Spilková, Jiřina (referee)
Charles University Faculty of Pharmacy Hradec Králové Department of Pharmaceutical Botany Candidate: Mgr. Eva Gorbunovová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Rigorous Thesis: Comparison of copper ion chelation by benzoic acids Copper is the third most widespread element in the human body. It is necessary to maintain the homeostasis of the organism, the proper functioning of enzymes, participate in various metabolic pathways. Benzoic acid and its derived acid belong to a group of phenolic compounds that are formed by the metabolic processing of flavonoids in the human body and can have antioxidant, anti-inflammatory and antitumor effects that could be used in medicine. In this rigorous thesis, the copper-chelating activity of six benzoic acids was tested at different pH values using spectrophotometric methods using hematoxylin and bathocuproinedisulfonic acid disodium salt as indicators. The top copper-chelating activity with cupric ions using hematoxylin and the bathocuproinedisulfonic acid disodium salt and copper-chelating activity with cuprous ions using bathocuproinedisulfonic acid disodium salt showed two acids, namely 2,4- dihydroxybenzoic acid and 2-hydroxy-4-methoxybenzoic acid. Key words: Phenolic acids, benzoic acid derivatives, copper, chelating, bathocuproinedisulphonic...
Poisonings with macromycetes in period 2001-2016
Komínková, Lucie ; Jahodář, Luděk (advisor) ; Karlíčková, Jana (referee)
The aim of this diploma thesis was to process an overview of previously known knowledge about acute mushroom poisonings. Thesis draws mainly from foreign literature. Provides basic information about mushrooms, their toxins and syndromes that cause. Beside the general information, one part of the thesis is devoted to the cases and studies from around the world. They describe individual clinical cases of mushroom poisoning or deal with their epidemiological situation mostly between the years 2001 - 2016. Obtained information is processed into overview tables. Thesis is particularly focused on the situation of macromycete poisonings in the Czech Republic and contains data from specialised facilities. The most intoxications occurs after ingestion of the edible fungi species. The most common intoxications by mushrooms containing toxins are after ingestion A. phalloides, A. pantherina and Psilocybe. The most frequent reported symptoms are gastrointestinal. According to the severity, mushroom poisonings are classified as less dangerous with onset of symptoms within 6 hours and life-threatening with the onset of symptoms after more than 6 hours. Mushroom poisonings should be considered in the differential diagnosis of gastrointestinal problems. Key words: macromycetes, mushroom poisonings, mushroom...
The effect of methylviologen on secondary metabolites production in in vitro culture of Fagopyrum esculentum, variety Bambi
Vlachová, Veronika ; Tůmová, Lenka (advisor) ; Karlíčková, Jana (referee)
10 ABSTRACT The subject of this study was to evaluate the efect of abiotc elicitor on rutn producton in callus and suspension cultures of buckwheat. The cultvar of buckwheat used for this research was Fagopyrum esculentum Moench var. Bambi, cultvated in Murashige and Skoog nutrient medium with the additon of growth regulator 2,4-dichlorfenoxyacetc acid (2,4-D) in concentraton of 1 mg/l. The elicitor used in this study was a soluton of methylviologen, 1 ml of it was added to the cultures in three diferent concentratons: c1 = 100.0 mg/100 ml, c2 = 10.0 mg/100 ml and c3 = 1.0 mg/100 ml. The elicitor was afectng the cultures for 6, 12, 24, 48, 72 or 168 hours. Afer the defned period of tme, cultures were collected, dried out and stored for further analysis of rutn content. To control samples (without elicitor treatment) 1 ml of ethanol 96% was added and they were collected afer 6, 24, 72 or 168 hours. Releasing of rutn into the nutrient medium was also investgated. Rutn content in each sample of cultures and in each sample of nutrient medium was later determined by HPLC. Any signifcant increase in the producton of rutn was not observed in this study. The maximum amount of rutn detected was 0.1 mg/g DW, thus the lowest quantty detectable, and was found in suspension cultures in three cases, afer the additon of...
Analysis of Ginger root extract. I.
Bačová, Barbora ; Jahodář, Luděk (advisor) ; Karlíčková, Jana (referee)
Bačová, B.: Analýza zázvorového extraktu. I. [Diplomová práce] Hradec Králové 2010, Univerzita Karlova v Praze, Farmaceutická fakulta. Komerční extrakt ze Zingiber officinale 8 : 1 byl analyzován na obsah gingerolu, vody a dalších látek. Byly použity chromatografické postupy včetně TLC a HPLC. Metodou hodnocení byly postupy uvedené v US Pharmacopeia 1996 a ČL 2009. Byla potvrzena přítomnost 6-gingerolu v množství 1,17%, obsah vody 89,18% a prokázané další látky podobné gingerolům.

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