National Repository of Grey Literature 15 records found  1 - 10next  jump to record: Search took 0.00 seconds. 
The culture of limbal and mesenchymal cells on various feeders for their use in ophthalmology.
Trošan, Peter ; Jirsová, Kateřina (advisor) ; Heissigerová, Jarmila (referee) ; Netuková, Magdaléna (referee)
P.Trošan Ph.D. Thesis Abstract Limbal stem cell deficiency (LSCD) is a disease characterized by the deficiency of stem cells in the limbus, which are responsible for the homeostasis and renewal of the corneal epithelium. This disorder results in corneal neovascularization, chronical inflammation and opacification, which may lead to loss of vision. The most successful treatment is the transplantation of limbal tissue or cultured limbal epithelial cells (LECs) onto the damaged ocular surface. The human amniotic membrane (HAM) is used as the feeder of the LECs culture, as well as for the LSCD treatment. HAM is also widely used in clinical practice, particularly for the treatment of chronic wounds. This dissertation is particularly concerned on cell therapy for LSCD, on preparation of cells suitable for grafting onto the ocular surface, on the improvement of the LECs culture conditions, and on the preparation of appropriate carrier for the transfer of cells onto the damaged cornea. During my work I have used a wide spectrum of methods, e.g. cell cultures (LECs, mesenchymal stem, amniotic epithelial, conjunctival epithelial, goblet and 3T3 cells), immunohisto- and immunocytochemistry, microscopy, proliferation and colony forming assays, reverse transcription and quantitative real-time PCRs and statistical...
Nutrition for oncological diseases
Strejčková, Vendula ; Jirsová, Kateřina (advisor) ; Aschermannová, Alexandra (referee)
Sufficient and quality nutrition is an important support for mastering serious oncological treatment and debilitating illness. Educating the public about the risk of oncological disease related malnutrition and the importance of a balanced diet, incidence of new malignanccies increases every year. 94 000 people as the newly diagnosis were in 2015. Nearly 542 000 people lived with cancer diagnosis in 2015. Not only among patient it is widespread idea that malnutrition is something that can not concern us because we are doing good. Many patients do not have insight into their nutritional status. They do not think they are at risk of malnutrition. But if someone gets cancer, sooner or later, the symptoms of the disease will appear, and along with demanding treatment that brings a lot of side effects, and worsing of oral intake, and weight loss is coming. The theoretical part describes the causes of the cancer disease, its influence on the organism, the most common types of anticancer treatment together with the side effects and the possibilities of their management. The second half of the theoretical work deals with the evaluation of nutritional risk in oncological patients, nutritional recommendations and in case of insufficient nutrition for any reason, as well as nutritional support. Objective...
The role and function of stromal enzymes in keratoconus pathogenesis
Ďuďáková, Ľubica ; Jirsová, Kateřina (advisor) ; Svozílková, Petra (referee) ; Ardan, Taras (referee)
Lubica Dudakova Doctoral Thesis ABSTRACT Keratoconus (KC) is a non-inflammatory disease of the cornea, in which ectasia and thinning occur probably due to defects in the collagen fibers binding. It is one of the most common indications for corneal transplantation. KC is a complex disorder with the involvement of both genetic and environmental factors; however the exact pathogenic mechanisms leading to the disease development have not been elucidated. The main aim of our work was to compare the presence and enzyme activity of cross- linking enzymes lysyl oxidases (LOX and LOX-like enzymes), in control human cornea samples and explanted cornea gained from patients with KC. We also focused on diseases previously described to be associated with KC with the aim to identify common signs among them. Furthermore, we replicated association of single nucleotide polymorphisms (SNPs) in LOX and hepatocyte growth factor (HGF) with KC risk. We attempted to link all pathophysiological disturbances observed in KC into one common pathway. We have used a wide spectrum of methods (cell culturing, immunohisto- and immunocytochemistry, microscopy, fluorimetric enzyme activity measurement, genotyping and direct sequencing, statistical analysis). We demonstrated the presence of entire family of LOX enzymes in control and in KC...
Sipping in the nutrition of oncologic pacients
Andrysíková, Šárka ; Aschermannová, Alexandra (advisor) ; Jirsová, Kateřina (referee)
Author: Andrysíková Šárka Institution: Univerzita Karlova v Praze, 1. lékařská fakulta Title: Sipping in the nutrition of oncologic pacients Tutor: as. MUDr. Alexandra Aschermannová Number of pages: 60 Number of supplements: 7 Year: 2015 Key words: Cancer, malnutrition, prevention, screening, nutritional support, parenteral nutrition, enteral nutrition, sipping This paper is focused on practical use of the simplest form of enteral nutrition - sipping - from the point of view of cancer patients. The aim of the paper is to find out whether nutritional intervention, namely recommendation of sipping is motivating enough for the patient or whether there is another factor that is important, i.e. a subjective attitude of the cancer patient - price, availability or flavor. The paper is divided into two parts - a theoretical part and a research one. The theoretical part describes terms - origin of cancer cells, statistics of cancer occurrence in the Czech Republic, the main ways of treating cancer. Diagnosis of malnutrition and evaluation of nutritional status, types of pharmaceutical enteral nutrition - tube feeding and sipping, parenteral nutrition. In the research part, there is discussed the reality of everyday use of sipping from the point of view of cancer patients.
Implementation of novel methods of tissue preparation and storage for transplantation purposes in ophthalmology
Rybičková, Ivana ; Jirsová, Kateřina (advisor) ; Vlková, Eva (referee) ; Čejková, Jitka (referee)
Introduction: Recent advances in posterior lamellar keratoplasty necessitated the preparation of posterior corneal lamellae even in the Czech Republic. The aim of this work was to introduce and standardize a novel method of manual preparation of corneal lamellae for Descemet Membrane Endothelial Keratoplasty with a Stromal rim (DMEK-S) in an ocular tissue bank. After setting the criteria for endothelial quality, we obtained a licence to provide the tissues for transplantation purposes. The obtained results were analysed after two years. Furthermore, a novel lamellar insertion technique using a cartridge was assessed. The potentials for the long-term storage of posterior corneal lamellae by the vitrification in liquid ethane was studied using human amniotic membrane as a model tissue. Material and Methods: Corneoscleral buttons stored in tissue cultures were used to prepare lamellae consisting of a central zone of endothelium and Descemet membrane supported by a stromal rim at the periphery. The manual preparation was performed on an artificial anterior chamber using the big bubble technique. Endothelial quality was assessed before storage, before and immediately after preparation as well as after 2 days of storage at 31řC. A group of 12 corneas with a live endothelial cell density ≥ 2500 cells/mm2...
Cell and Molecular Characterization of Failed Human Corneal Grafts. The Role of Matrix Metalloproteinases in Recurrent Corneal Melting.
Brejchová, Kristýna ; Jirsová, Kateřina (advisor) ; Smetana, Karel (referee) ; Heissigerová, Jarmila (referee)
The aim of this work was to investigate the contribution of matrix metalloproteinases (MMPs) to recurrent corneal melting. Twenty three melted corneas from seven patients were separated into three groups: a) patients with primary Sjögren's syndrome, b) those with rheumatoid arthritis and c) those with other corneal melting underlying pathologies. Eleven cadaverous corneas served as controls. The presence of MMP-1, -2, -3, -7, -8, -9, and -13 was detected using indirect enzyme immunohistochemistry. The active forms of MMP-2 and -9 and MMP- 3 and -7 were examined by gelatin and casein zymography, respectively. The concentrations of active MMP-1 and -3 were measured using activity assays. Increased immunostaining intensity for MMP-1, -2, -3, -7, -8 and -9 was shown in the corneal epithelium and the stroma of almost all melted corneas from all three groups compared to the negative or slightly positive staining of the controls. In the endothelium, immunostaining for MMP-2 and MMP-9 was increased in most specimens of groups II and III and group I, respectively. A markedly higher level of active MMP-2 was detected in six, and active MMP-9 in all, pathologic specimens compared to the controls. In contrast to the completely negative controls, the proenzymes of MMP-3 and -7 were detected in almost all melted...

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See also: similar author names
1 Jirsová, Kamila
2 Jirsová, Karolína
1 Jirsová, Klára
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