National Repository of Grey Literature 7 records found  Search took 0.01 seconds. 
Parametrization of network attacks
Jelínek, Michael ; Sikora, Pavel (referee) ; Blažek, Petr (advisor)
This bachelor thesis is dedicated to the definition of suitable parameters for network attack identification with the use of neural networks. In the theoretical part of this thesis are methods for anomaly detection in network communication, structure of artificial neural networks and DDoS attacks used for verification of detection capabilities. The practical part of this thesis is focused on the process of preparing data, the subsequent implementation into neural networks and a summary of the results achieved for the different setups of neural networks.
Molecular mechanisms of apoptosis induction by taxanes in breast cancer cells
Jelínek, Michael ; Kovář, Jan (advisor) ; Brábek, Jan (referee) ; Reiniš, Milan (referee)
Taxanes are cytostatic routinely used for the treatment of solid breast, ovarian, prostate, head and neck tumors and other types of tumors. Resistance of tumor cells to the effect of taxanes represents serious obstacle for the employment of taxanes in the treatment of tumors. This resistance can be associated, among other things, with lower rate of apoptosis induction in cancer cells or also with increased level of transporters transporting taxanes out of the cell. In this PhD thesis we tried: (1) to contribute to elucidation of the role of molecular mechanisms of apoptosis induction by taxanes in cells of human breast cancer. Specifically, it meant to contribute to elucidation of the role of initiator caspase -8 a - 9 and mainly of initiator caspase-2. Next, to contribute to elucidation of the role of executioner caspase -3 - 6, and -7 and selected proteins of the Bcl-2 family. (2) To contribute to elucidation of molecular mechanisms of resistance of human breast cancer cells to taxanes. Specifically, it meant to describe the role of selected functional groups in taxane structure in bringing about and overcoming resistance to taxane and next to contribute to elucidation of the role of P-glycoprotein (ABCB1 transporter) in the resistance to individual taxanes. 1) We found that caspase-2 represents...
The role of autophagy and selected beta-tubulin isotypes in taxane resistance in breast cancer cells
Kábelová, Adéla ; Jelínek, Michael (advisor) ; Truksa, Jaroslav (referee)
Drug resistance in cancer cells is a frequent cause of breast cancer therapy failure. The aim of this thesis was to elucidate mechanisms of resistance to taxanes, that are used in therapy of various types of cancer, including breast cancer. We particularly assessed the role of autophagy and changes in βII- and βIII isotype gene expression in development of taxane resistance. As model of breast cancer we used human sensitive cell lines SK-BR-3, MCF-7 a T47-D and resistant sublines SK-BR-3-PAC/REZ a MCF-7- PAC/REZ which grow in paclitaxel concentration lethal for sensitive sublines. In cell lines SK-BR-3 and MCF-7, taxane application decreased the level of autophagy, however in cell line T47-D led to its activation. We detected no difference between basal levels of autophagy in sensitive subline SK-BR-3 compared to resistant subline SK-BR-3-PAC/REZ, but we observed increased basal level of autophagy in sensitive subline MCF-7 compared to the resistant subline. Increase or decrease level of autophagy did not affect taxane resistance, except activation of autophagy in resistant subline SK-BR-3-PAC/REZ, that further increased the resistance to paclitaxel. Taxane application in cell line T47-D increased the levels of βII- and βIII-tubuline expression, however we did not find any similar effect in other tested...
Induction and course of programmed cell death in cancer cells after taxane application."
Kábelová, Adéla ; Jelínek, Michael (advisor) ; Gemperle, Jakub (referee)
The taxanes are a class of commonly used anticancer agents, which are very effective in treatment of breast, ovarian, prostate or lung cancer. Taxanes bind to the β-tubulin subunit of microtubules and lead to their stabilization and inhibition of depolymerization. Such microtubules lose their function to form mitotic spindle, thus arresting cells in G2/M phase and resulting in apoptosis. Unfortunately some cells are able to escape from taxanes-induced apoptosis by developing various mechanisms of resistance including alteration in taxanes target microtubules or upregulation of specific transporters that pump the drug out of cells. Other types of resistance are connected with process of programmed cell death (PCD), especially with proteins that after taxane application participate in its successful progress. Taxanes can directly or indirectly modify the activity of Bcl-2-family proteins that control mitochondrial and endoplasmic reticulum integrity, thus regulating the initiation of PCD. Caspases are executioners of PCD and caspase-2 activated by cytoskeletal disruption seems to be especially important in taxanes- induced apoptosis. In some cases can taxane treatment also result in caspase-independent cell death. Special role has protein p53 that seems to be involved only in apoptosis caused by low taxanes...
Vaccination - yes or no? Public perception of the benefits of vaccines
Chudomelová, Iva ; Drda Morávková, Alena (advisor) ; Jelínek, Michael (referee)
Thanks to the vaccination were saved millions of lifes around the world, but this topic raises some controversy. Many European countries abolished the obligatory tuberculosis vaccination. The main argument was steadily decreasing incidence of infectious diseases and burdening BCG vaccination for the newbors and more adverse reactions detected in recent years. The cause of their formation is probably connected with other antigens. The downtrend in the incidence of TB is recorded only in some states, mainly in Western Europe. With respect to the current demographic situation and legal or illegal migration, it is continue necessary to take this disease seriously and to investigate the new vaccination strategies.

See also: similar author names
24 JELÍNEK, Martin
2 Jelínek, M.
5 Jelínek, Marek
2 Jelínek, Marian
24 Jelínek, Martin
10 Jelínek, Michal
2 Jelínek, Milan
6 Jelínek, Miroslav
1 Jelínek, Mojmír
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