National Repository of Grey Literature 282 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
In vitro susceptibility of potentially pathogenic fungi isolated in the University Hospital Hradec Králové to antifungal drugs.
Hejkrlíková, Eva ; Jílek, Petr (advisor) ; Janďourek, Ondřej (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Author: Eva Hejkrlíková Supervisor: PharmDr. Petr Jílek, CSc. Supervisor specialist: doc. RNDr. Vladimír Buchta, CSc. Title of master's thesis: In vitro susceptibility of potentially pathogenic fungi isolated in the University Hospital Hradec Králové to antifungal drugs Subtitle: Blood cultures tested from 2008 to 2018 Background: The aim of the work was to describe and evaluate epidemiological situation of fungemia in the University Hospital Hradec Králové (FNHK) from 2008 to 2018, focusing on in vitro susceptibility of blood isolates to antifungals. Methods: Required data were obtained from the laboratory information system of the De- partment of Clinical Microbiology FNHK and processed using Microsoft Excel program. Results: From January 2008 to December 2018, fungemia was identified in 231 patients with overall incidence of 0.51 cases per 1000 admissions. Candida albicans accounted for most cases (52.8 %) followed by C. glabrata (14.7 %), C. tropicalis (8.2 %) and C. parapsilosis (6.9 %). C. albicans, C. tropicalis and C. parapsilosis susceptibility to echinocandins, as determined on the basis of minimum inhibitory concentration, was 100 %, in 2 cases C. glabrata was resistant to anidulafungin...
Evaluation of selected biomarkers for early detection of intraamnial inflammation in women with preterm premature rupture of fetal membranes (PPROM)
Bukáčková, Nella ; Jílek, Petr (advisor) ; Souček, Ondřej (referee)
Nella Bukáčková Evaluation of selected biomarkers for early detection of intraamnial inflammation in women with preterm premature rupture of fetal membranes (PPROM) Diploma thesis Charles University, Faculty of Pharmacy in Hradec Králové Specialist in Laboratory Methods Background: The aim of this work is to evaluate selected parameters of innate immunity and inflammation in connection with their clinical use in early prediction of premature labor complicated with premature rupture of fetal membranes (PPROM). Potential biomarkers include the following components of the complement system: C3 component, activated C3 component (C3a), C5 component and activated C5 component (C5a). The regulatory protein of the complement system, the protectin (CD59) was also added to the list. Methods: The study was conducted with a group of 136 patients selected according to specific criteria such as a single pregnancy or PPROM. Using immunochemical methods, the concentration of interleukin 6 in amniotic fluid was determined and bacterial nucleic acid was detected by microbial colonization of the amniotic cavity by a polymerase chain reaction. Enzyme immunoassay (ELISA) was chosen for the analysis of selected components of the complement. Results: There was a statistical difference in C3, C3a, and CD59 concentrations...
In vitro screening of potential antimycobacterial compounds against fast growing strains of Mycobacterium genus II
Pchálková, Tereza ; Janďourek, Ondřej (advisor) ; Jílek, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Study program: Specialist on Laboratory Methods Author: Bc. Tereza Pchálková Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title of diploma thesis: In vitro screening of potential antimycobacterial compounds active against fast growing strains of Mycobacterium genus II Key words: Mycobacteria, Tuberculosis, Antituberculotics, Microdilution broth method, Minimum inhibition concentration Background: The aim of this diploma thesis has been screening of in vitro antimycobacterial activity of novel compounds against fast growing strains of the genus Mycobacterium (Mycobacterium smegmatis and Mycobacterium aurum). Another aim has been predicting the structure-activity relationships for tested compounds. Methods: The technique used for activity determination was microdilution broth method. The value of minimum inhibition concentration for each compound was determined. The evaluation was performed visually and spectrophotometrically using the Alamar blue indicator. Results: A total of 79 compounds were tested. 22 tested compounds showed significant activity against mycobacteria. The substances were sorted according to the similarity in chemical structure into 10 groups. From a chemical point of view, these...
Immunopharmacology and immunotherapy of cancer
Havlíková, Kateřina ; Rathouská, Jana (advisor) ; Jílek, Petr (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Consultant: PharmDr. Jana Rathouská, Ph.D. Candidate: Kateřina Havlíková Title of diploma thesis: Immunopharmacology and immunotherapy of cancer Main goal of this work is to summarize modern trends of immunopharmacology and immunotherapy of tumour disease and to stress their importance for the future of therapy. To add context to immunotherapy there are also described characteristics of tumour disease and immune system, on which many principles of immunotherapy are directly based. This diploma thesis is divided into three individual parts - carcinogenesis, immune system and immunotherapy. The carcinogenesis chapter includes basic mechanism of cancer development, principles how body protects itself against tumour disease and mechanisms how this protection fails. In the immune system chapter there are described individual types of immunity and mechanism by which immunity fails in the case of tumor disease. The immunotherapy chapter describes recent findings of how the properties of immune system can be utilized to combat tumor disease. Approved and used therapies are included.
Factors related to chronic vulvovaginal discomfort - questionnaire study in Slovakia
Štibľaríková, Adriána ; Jílek, Petr (advisor) ; Leško, Daniel (referee)
Autor: Adriána Štibľaríková Title: Factors related to chronic vulvovaginal discomfort - questionnaire research in Slovakia Graduation thesis Charles University, Faculty of Pharmacy in Hradec Králové Field of study: Pharmacy OBJECTIVE: A very common and quality of life reducing disease is vulvovaginal discomfort (VVD), which affects more than two thirds of women at least once in a lifetime. With recurring vulvovaginal discomfort we talk about recurrent vulvovaginal discomfort (RVVD), which affects the patient at least 3 times a year, or 4 times in a lifetime. The aim is to search a group of women who have been diagnosed with RVVD and healthy women and/or women with VVD and compare them. We compare a group with RVVD and the theoretical hypotheses obtained by examining and long-term studies. We compare women based on age, the number of sexual partners, the impact of food and lifestyle on formation of vaginal problems and the development of chronic vulvovaginal discomfort. The evaluated group consisted of 212 respondents, 46 from the RVVD group and 166 from the control group. METHODS: We obtained data for the elaboration of this graduation thesis using a questionnaire, which consisted of 45 questions, where respondents could mark one or more options, in several questions they could express themselves...
Monoclonal gamopathy
Semerádová, Eva ; Nachtigal, Petr (advisor) ; Jílek, Petr (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Medical Laboratory Technician Candidate: Eva Semerádová Thesis supervisor: Prof. PharmDr. Petr Nachtigal, Ph.D. Consultant: Jana Šimková, MSc. Title of bachelor thesis: Monoclonal gammopathy The aim of this thesis is to describe monoclonal gammopathy (MG) The bachelor thesis describes forms of this disease, its history, classification and variants of laboratory diagnosis. Interesting. Monoclonal gammopathy is characterized by the presence of monoclonal immunoglobulin in serum or urine. There are two main types of the disease - malignant monoclonal gammopathy (MMG) and monoclonal gammopathy of undetermined significance (MGUS). For the diagnosis of MG is essential to determine the presence of monoclonal immunoglobulin in serum or urine and to determine the extent of bone marrow lesion. Based on the risk factors (radiation, chemical carcinogens, genetic predisposition, virus dual intervention-theory) were taken into diagnostic tests as well those describing free light chains ratio, number of malignant cells and quantity of monoclonal immunoglobulin. The most commonly used diagnostic methods are electrophoretic methods because of their time- and cost-effectiveness. For the typing is...
Phylogenetic diversity of culturable prokaryotes from hypersaline environments.
Straková, Dáša ; Jílek, Petr (advisor) ; Konečná, Klára (referee)
Candidate: Dáša Straková Title of diploma thesis: Phylogenetic diversity of culturable prokaryotes from hypersaline environments. Charles University, Faculty of Pharmacy Department of Biological and Medical Sciences University of Sevilla, Faculty of Pharmacy Department of Microbiology and Parasitology Study program: Pharmacy Hypersaline environments are widely distributed habitats characterized by elevated concentrations of salts, mainly represented by aquatic systems and hypersaline soils. Their study has enabled the isolation and characterization of a large number of halophilic microorganisms during the last decades, which are microorganisms that require a high concentration of salts in order to be capable of life and growth. The aim of this work was to continue the initial characterization of halophilic Archaea and Bacteria, previously isolated by Ana Durán-Viseras in 2016 from several water samples from Isla Cristina and Isla Bacuta solar salterns (Huelva, Spain) and from a hypersaline soil located in Odiel Saltmarshes (Huelva, Spain), and to carry out a biodiversity study of these samples. Ana Durán-Viseras started this project and I continued with it during my stay in Sevilla. As a part of the characterization, we sequenced the partial 16S rRNA gene of the isolated strains. For those that...
Voluntary vaccination in children
Sejkorová, Ilona ; Rathouská, Jana (advisor) ; Jílek, Petr (referee)
Bc. Ilona Sejkorová Voluntary vaccination in children Charles University in Prague, Faculty of Pharmacy in Hradec Králové Specialist in laboratory methods Many viruses or bacteria may be the cause of infectious diseases. Is possible to use vaccination against some of them. Voluntary vaccination in childeren is still an actual topic. Even though, vaccination has many opponents, it still belongs to the most effective preventive precautions. Objectives: The thesis consists of a theoretical summary of information related to child voluntary vaccination problems. The practical part consists of a questionnaire study whose goal was to determine the attitude of parents and potential parents to child optional vaccination topic. Also, to find out the awareness of the population about the topic, to assess the most used voluntary vaccination, and possibly further information about dangerous side effects of the voluntary vaccinations. Methods: A questionnaire study for a period October 2018 to February 2019 in the Czech Republic. The questionnaire consisted of 20 questions directed to parents and potential parents of various ages (606 respondents). Results: Most of the respondents (94 %) were aware about a possibility of the child voluntary vaccination. More than a half (51 %) of respondents was informed about...

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