National Repository of Grey Literature 7 records found  Search took 0.00 seconds. 
Inducible expression systems and their use in the study of parasitic organisms.
Horáčková, Vendula ; Doležal, Pavel (advisor) ; Fišer, Radovan (referee)
1 Abstract Inducible expression systems are systems with ability to switch expression of genes of interest on and off. Therefore, they are useful molecular tools for analysis of gene function. Nowadays, there are tens of various inducible expression systems available that differ from each other in level of regulation of gene expression, time of induction, possibilities of use, etc. This work is focused on three of them to illustrate common features of the inducible expression systems which regulate gene expression at the level of transcription. Firstly, systems based on regulation of lactose operon of Escherichia coli are mentioned. Secondly, systems which use regulatory elements of tetracycline resistance-encoding transposon Tn10 of E. coli are described. Third chapter is focused on systems regulated by agonists of ecdysone receptor. In the last chapter cases of use of inducible expression systems in the study of parasitic organisms are summarized.
Molecular diagnosis of viral hepatitis C - personal experience from routine laboratory
Horáčková, Veronika ; Vejsová, Marcela (advisor) ; Fajfr, Miroslav (referee)
Author: Veronika Horáčková Title: Molecular diagnosis of viral hepatitis C - personal experience from routine laboratory Bachler work Field of study: Medical laboratory technician, combined study form The aim of the study: Introduction to the issues concerning the viral Hepatitis C with an emphasis on the laboratory diagnostics thereof. Statistical processing of the Hepatitis C virus genotype analysis results. Material and methods: In the period of 1 July 2005 - 30 June 2012, the Hepatitis C virus genotype analysis was performed in respect of 2 185 samples at the division of molecular biological diagnostics of the serology laboratory of the Institute of Clinical Biochemistry and Laboratory Diagnostics of the General Faculty Hospital and the First Faculty of Medicine of Charles University in Prague, using the reverse hybridization method. The testing results were statistically processed. Results: Of the set of 2 185 samples, 1 372 (62,8 %) originated from males and 813 (37,2 %) from females, with the average age of patients included in the set being 36,7 years. The majority of requests for the HCV genotype analysis originated from specialists in Prague (91,0 %). The genotype analysis demonstrated the highest prevalence of the 1b genotype (51,6 %), followed by the 3a genotype (22,6 %). The prevalence...
Socio-economic impacts of the strategic industrial zones on the development of the region
Horáčková, Veronika ; Damborský, Milan (advisor) ; Hornychová, Taťána (referee)
The aim of this thesis is to define socio-economic impacts of the strategic industrial zones on the development of the region. The aim of the thesis was chosen with respect to the statement from literature, that industrial zones stimulate economic activity through making new jobs. In the first part of work are described teoretical fundamentals present by Czech and also foreign authors, who are focused on industrial zones problematics, characteristics of industrial zones in the Czech Republic and localization. In the second part are two case studies of strategic industrial zones Most-Joseph and Ostrava-Mošnov. The component of case studies is analysis of statistics for the period from 2000 to 2012, which results from observation of casual relations between development of zones and their surrounding areas lead to identification of economic and social impacts of strategic industrial zone on the development of the region.
Trafficking in human beings as a way of strengthening the market competitiveness of the Czech Republic?
Horáčková, Veronika ; Gráf, Václav (advisor) ; Chalupníček, Pavel (referee)
The thesis considers trafficking as a way of strengthening the market competitiveness of the Czech Republic. Contrary to wide-spread approach to this issue, only as to violation of the rights of victims, trafficking is examined from the side of entrepreneurs and from the side of impact on national competitiveness. In addition to factors affecting the national economy and the motivation of entrepreneurs is evaluated the efficiency of subsidies provided by the Ministry of the Interior to Programme of the support and protection for victims of trafficking. In the thesis is found out that the exploitation of trafficked persons increases competitiveness of the Czech Republic, because it fills gaps on the domestic labour market with no wage increases. For employers victims are flexible and low-cost source of labour force, which increases productivity of their firms, but they don't represent significant competition for legal workers in the country.
Potato containing a transgene for the fusion protein SPI2::GFP
Navrátil, Oldřich ; Kludkiewicz, Barbara ; Vinokurov, Konstantin ; Horáčková, V. ; Sehnal, František
Plant breeding is facing a new era, the horizons of which are determined by transition from studies of the role of individual genes in biological processes to global search for the role of genome and proteome in determination of biological processes. Thegeneral aim of the center is to utilize the potential of the functional genomics and proteomics to obtain novel information and breeding material to increase the quality of agricultural products, specifically, to improve the agronomic parameters and modifying specific properties of crop plants important not just in the Czech Republic, but also in Central Europe in general. Research will focus on increasing of (i) quality of wheat and barley corn, pea seeds and potato tubers, (ii) potato and pea resistance to pests and pathogenes, and (iii) heavy metal uptake and resistance in flux for phytoremediation.

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1 Horáčková, Vendula
4 Horáčková, Veronika
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