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Biological aktivity of secondary plants metabolites X. Alkaloids of Vinca minor L.
Šípková, Pavla ; Hošťálková, Anna (advisor) ; Chlebek, Jakub (referee)
Šípková, P.: Biological activity of secondary plants metabolites X. Alkaloids of Vinca minor L. Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany, Hradec Králové, 2019, 66 s. Key words: Apocynaceae, Vinca minor, alkaloids, isolation, biological activity, screening Alzheimer's disease is a progressive neurodegenerative disease. The number of affected patients is constantly increasing. This disease cannot be treated casually, therefore discovering and testing new substances that could potentially be used in a treatment is very important. The Vinca minor L. fraction after column chromatography was separated by flash chromatography. Isolation of the individual alkaloids was performed by preparative TLC. Based on NMR and MS analyses and comparison with literature, alkaloids were identified as vincarubine and (-)-vinoxine. Modified Ellman's method was used to test cholinesterase inhibitory activity of isolated alkaloids. Acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) are enzymes, that play a very important role in the pathofysiology of Alzheimer's diasease. (-)-Vinoxine showed relatively high activity against BuChE (IC50 = 24,61 ± 1,71 µM), inhibitory activity against AChE was insignificant (IC50 > 1000µM). Vincarubin did not...
Bioactive metabolites of representative members in the genus Magnolia
Lakatošová, Ivana ; Hošťálková, Anna (advisor) ; Opletal, Lubomír (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany Candidate: Ivana Lakatošová Supervisor: PharmDr. Anna Hošťálková, Ph.D. Title of Master's thesis: Bioactive metabolites of representative members in the genus Magnolia 2019, 72 p. Key words: Magnolia, secondary metabolites, traditional medicine, CNS bioactivity The aim of this Master's thesis was to summarise current knowledge of significant secondary metabolites of species Magnolia officinalis and Magnolia obovata, and their biological activity. Botanical features of genus Magnolia were defined and described, followed by the classification of bioactive compounds isolated from species Magnolia officinalis and Magnolia obovata. The Master's thesis focused predominantly on the effects of biphenol compounds of magnolol and honokiol, which have been subjected to many studies. The main attention was given to the immunosuppressive effect and pharmacologic effects on the nervous system. Essential oils were observed to have an anti-inflammatory and a myorelaxant effect. Alkaloids and neolignans, which had been known to relax spasms of skeletal muscles, expressed neuroprotective activity. Neolignans were shown to have the inhibitory effect on bacteria, viruses and pathogenic fungi. They also proved themselves to be...
Alkaloids of family Amaryllidaceae: genus Hippeastrum
Öhlschlegelová, Jana ; Hošťálková, Anna (advisor) ; Šafratová, Marcela (referee)
Author: Jana Öhlschlegelová Title: Alkaloids of family Amaryllidaceae: genus Hippeastrum Diploma thesis Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany 2019, 74 p. Keywords: Hippeastrum, Amaryllidaceae, alkaloids, antiproliferative activity, Alzheimer's disease, cholinesterase inhibitors, galanthamine The aim of this diploma thesis was to unify current findings about alkaloids isolated from selected plants of the Hippeastrum genus in the Amaryllidaceae family. The fytochemical characteristics of the examined species was introduced, and a group of alkaloids which were isolated from these plant species was composed. Also, the biological activity was evaluated. Up to date, at least 13 plant species of the Hippeastrum genus were examined from the fytochemical perspective. Out of these species examined, 56 different alkaloids with defined structure were isolated. The isolated alkaloids are divided into several groups based on their structure. Namely, these are lycorine, homolycorine, crinine, galanthamine, narciclasine, tazettine, haemanthamine and montanine structural types. Also, alkaloids which differed structurally from these basic types were found in several plants studied. In the substances gained, the antiproliferative activity, inhibitory activity...
Alkaloids from the wood of the species Liriodendron tulipifera L. and their activity against human cholinesterases
Hrušková, Magda ; Hošťálková, Anna (advisor) ; Chlebek, Jakub (referee)
Hrušková M.: Alkaloids from the wood of the species Liriodendron tulipifera L. and their activity against human cholinesterase. Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany, Hradec Králové, 2018. Key words: Liriodendron tulipifera, alkaloids, biological activity. The alkaloids from the wood of the species Liriodendron tulipifera L. were isolated and their inhibitory activity against acetylcholinesterase, butyrylcholinesterase and propyl oligopeptidase, which are enzymes involved in the pathophysiology of Alzheimer's disease (AD), was investigated. The search and testing of new substances, which are used in AD treatment, is very relevant, as this disease cannot be casually treated yet. An alkaloid extract of Liriodendron tulipifera L. wood was tested in a preliminary testing for inhibitory activity against human cholinesterase. Because of the promising results, it was chosen for an isolation and identification of possible effective alkaloids. The extract was carried out by column chromatography with a step gradient elution. A preparative TLC was used to isolate alkaloids. The identification of alkaloids was done by structural analyses (NMR and MS). Optically active substances were measured for their optical rotation. A modified...
Alkaloids of family Amaryllidaceae: genus Nerine.
Hodulová, Adéla ; Cahlíková, Lucie (advisor) ; Hošťálková, Anna (referee)
Author: Adéla Hodulová Title: Alkaloids of Amaryllidaceae family, genus Nerine Diploma thesis Charles University, Faculty of pharmacy in Hradec Králové, Department of Pharmaceutical botany 2018, 51 p. The aim of this diploma thesis was to summarize up the findings about alkaloids which where isolated from genus Nerine plants of Amaryllidaceae family. There was introduced a botanical characteristic of the phytochemical studied species of the genus Nerine. The most studied species was Nerine bowdenii. All the knowledges about mentioned alkaloids were sum up. Ten species have been phytochemical studied out of the genus Nerine until now. In total, 40 alkaloids were isolated from these plants. Alkaloids which where isolated from the Nerine are divided into several structural types. Anticancer, acetylcholinesterase-inhibitory of the alkaloids were described. The biological activity is connected with their stucture. The most significant anticancer activity was observed in alkaloids from crinine- and lycorine type . Acetylcholinesterase-inhibitory activity was pronounced the most in crinine-type alkaloids. Keywords: Nerine, Nerine bowdenii, Amaryllidaceae, alkaloids
Alkaloids from wood of the species Liriodendron tulipifera L. and their biological activity
Klíčová, Anna ; Hošťálková, Anna (advisor) ; Chlebek, Jakub (referee)
Klíčová A.: Alkaloids from wood of the species Liriodendron tulipifera L. and their biological activity. Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department: Pharmaceutical Botany, Hradec Králové, 2018. Supervisor: PharmDr. Anna Hošťálková, Ph.D. Key words: Liriodendron tulipifera, secondary metabolites, alkaloids, biological activity Alzheimer's disease (AD) is the most common type of dementia. It is neurodegenerative disorder. Patients suffer from cognitive disorder in combination with aphasia, apraxia or disorders of executive functions, which leads to exacerbation of working or social skills. Nowadays, there exist no casual treatment for AD, and that is why the intense research is still waging. Currently there are available remedies, which can slow down progress of AD. These drugs includes also natural substances. An alkaloid extract of Liriodendron tulipifera L. wood showed promising inhibition of human cholinesterases in a preliminary testing. These results were the reason why this extract has been chosen for further isolation of single alkaloids and identification of their biological activity. Isolation was made by column chromatography with step gradient elution. Then was used preparative TLC to isolate single alkaloids. Identification of alkaloids was...
Alkaloids from the wood of the species Liriodendron tulipifera L. and their activity against human cholinesterases
Hrušková, Magda ; Hošťálková, Anna (advisor) ; Chlebek, Jakub (referee)
Hrušková M.: Alkaloids from the wood of the species Liriodendron tulipifera L. and their activity against human cholinesterase. Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany, Hradec Králové, 2018. Key words: Liriodendron tulipifera, alkaloids, biological activity. The alkaloids from the wood of the species Liriodendron tulipifera L. were isolated and their inhibitory activity against acetylcholinesterase, butyrylcholinesterase and propyl oligopeptidase, which are enzymes involved in the pathophysiology of Alzheimer's disease (AD), was investigated. The search and testing of new substances, which are used in AD treatment, is very relevant, as this disease cannot be casually treated yet. An alkaloid extract of Liriodendron tulipifera L. wood was tested in a preliminary testing for inhibitory activity against human cholinesterase. Because of the promising results, it was chosen for an isolation and identification of possible effective alkaloids. The extract was carried out by column chromatography with a step gradient elution. A preparative TLC was used to isolate alkaloids. The identification of alkaloids was done by structural analyses (NMR and MS). Optically active substances were measured for their optical rotation. A modified...
Alkaloids of Papaver rhoeas L. (Papaveraceae) and their biological activity related to Alzheimerʼs disease II.
Čakurdová, Marta ; Chlebek, Jakub (advisor) ; Hošťálková, Anna (referee)
Čakurdová, M.: Alkaloids of Papaver rhoeas L. (Papaveraceae) and their biological activity related to Alzheimer's disease II. Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové, 2017. The aim of this work was focused on isolation of alkaloids from the fraction 1 (joined fractions 20-27), which was obtained from the summary alkaloid extract of Papaver rhoeas L. (Papaveraceae). The alkaloids were identified by structural analysis NMR, GC-MS, optical activity and melting point. Two alkaloids were identified as (-)-stylopine and (+)-rhoeadine. Isolated substances were tested on ability to inhibit the enzymes acetylcholinesterase, butyrylcholinesterase and prolyloligopeptidase. Obtained data were expressed as IC50 values: (-)-stylopine (IC50 AChE = 522 ± 67 µM, IC50 BuChE = >1000 µM, IC50 POP = >790 µM); (+)-rhoeadine (IC50 AChE = 915 ± 64 µM, IC50 BuChE = >1000 µM, IC50 POP = >790 µM). None of the isolated substances showed so potent cholinesterase inhibitory activity as the alkaloids galanthamine (IC50 AChE = 1.71± 0.065 µM, IC50 BuChE =42.30 ± 1.30 µM) and huperzine A (IC50 AChE = 0.033 ± 0.001 µM, IC50 BuChE = >1000 µM) or rivastigmine (IC50 AChE = 0.037 ± 0.001 µM, IC50 BuChE = 3.3 ± 0.3 nM)....
Alkaloids of family Amaryllidaceae: genus Zephyranthes
Jánská, Lucie ; Šafratová, Marcela (advisor) ; Hošťálková, Anna (referee)
Author: Lucie Jánská Název: Alkaloids of family Amaryllidaceae: genus Zephyranthes Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmacognosy 2018, 75 p. The aim of this diploma thesis was to summarize all knowledge about alkaloids izolated from Zephyranthes plants of Amaryllidaceae family. It contains a detail overview of botanical charactericts of phytochemically studied plants of the genus Zephyranthes. Also the overview of alkaloids with bilogical activity was described. Within the genus Zephyranthes 10 species were studied phytochemically and 89 alkaloids were isolated from this plants. This alkaloids are divided in several structural groups. The lycorine-, haemanthamine-, galanthamine- and pancratistatine-type alkaloids occure the most frequently. Acetylcholinesterase-inhibitory, anticancer and antimalarial activity of the alkaloids was described like the most important. The most significant acetylcholinesterase- inhibitory activity was observed in alkaloids from galanthamine sctructural type. Anticancer activity was found the most in lycorine-, pancratistatine- and haemanthamine- type alkaloids. The most notable antimalarial activity was observed in lycorine- and heamanthamine- type alkaloids. Keywords Zephyranthes, Amaryllidaceae,...
Isolation of alkaloids of the species Magnolia soulangeana Soul.-​Bod. and study of their biological activity
Baková, Izabela ; Hošťálková, Anna (advisor) ; Opletal, Lubomír (referee)
Baková I.: Isolation of alkaloids of the species Magnolia soulangeana Soul.-Bod. and study of their biological activity. Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové, 2017. Key words: Magnolia solulangeana, secondary metabolites, alkaloids, biological activity. Secondary metabolites of plants are responsible for various biological activities. Alkaloids were described as a potentially suitable for Alzheimer's disease therapy (AD) through their inhibition activities against cholinesterases. Nowadays, these substances are important medicine for AD, therefore a screening of herbal drugs is still a current topic. An alkaloid extract of Magnolia × soulangeana flowers was tested in a preliminary testing on anticholinesterase activity. Because of the promising results, it was chosen for an isolation and identification possible effective alkaloids. The extract was separated by a column chromatography using aluminium oxide and a step gradient elution. Alkaloids were isolated by a repeated preparative thin-layer chromatography. Individual alkaloids were identified by a structural analysis (NMR, MS) and then their optical activity was measured. Substances were tested for an inhibition activity against human...

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