National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Electrochemistry as a Tool for an Enzyme Characterization
Gál, M. ; Krahulec, J. ; Jiríčková, K. ; Sokolová, Romana ; Híveš, J.
Basic biochemical properties such as Michaelis constant (KM) and turnover number (kcat) of enzyme enteropeptidase was measured by electrochemical impedance spectroscopy. Both values determined by impedance measurements are in good agreement with those obtained by traditional spectroscopic techniques. Therefore one can suppose that electrochemical methods might be successful for such measurements also in the case where usual ones (UV-Vis, fluorescence spectroscopy) are not able to determine these enzyme characteristics.
Properties of some radiosensitizers studied by means of the electrochemical methods
Gál, Miroslav ; Kolivoška, Viliam ; Ambrová, M. ; Híveš, J. ; Sokolová, Romana ; Hromadová, Magdaléna
To determine the correlation between reduction potential and some physicochemical properties of selected radiosensitizers cyclic voltammetry and theoretical calculations based on the DFT method with B3LYP functional of the level of 6-311++G** basis set in vacuum were utilized. Very good correlation was found between electron affinities of radiosensitizers and their reduction potential and so called E1(7) potential.
Electrochemistry of selected radiosenzitizer-etanidazole
Gál, Miroslav ; Híveš, J. ; Sokolová, Romana ; Hromadová, Magdaléna ; Kocábová, Jana ; Kolivoška, Viliam ; Pospíšil, Lubomír
The first electron transfer to radiosensitizer etanidazole (ETN) and ETN radical anion formation in buffered aqueous media was studied by means of voltammetric techniques and Electrochemical Impendance Spectroscopy (EIS). The heterogeneous electron transfer rate constant for the first reduction of ETN (radical anion production) k0 for the redox couple and so called E1,7 potential were calculated. The obtained values of k0 and E1,7 potential indicate that ETN compared to some other possible chemical radiosensitizers requires lower energy to accept the first electron during metabolic pathway.

Interested in being notified about new results for this query?
Subscribe to the RSS feed.