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Synthesis of spherical gold nanoparticles for biomedical applications
Gablech, Evelína ; Adam, Vojtěch (oponent) ; Drbohlavová, Jana (vedoucí práce)
This paper deals with synthesis of spherical gold nanoparticles for various biomedical applications. Gold nanoparticles were synthetized by green methods. Considerable part of this thesis is devoted to green procedures. The aim of this work was to synthetize stable colloid gold nanoparticles suitable for various biomedical applications; especially for in vivo and in vitro bioimaging techniques. Review of these methods is included in theoretical part of this thesis as well. A cytotoxicity test was also provided. Gold nanoparticles were characterized by methods SEM, DLS, UV-VIS.
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Ion-selective membrane with antimicrobial effect
Gablech, Evelína ; Farka,, Zdeněk (oponent) ; Soukupová,, Jan (oponent) ; Drbohlavová, Jana (vedoucí práce)
This work deals with improvement of antimicrobial properties of commercial polymer membranes used in dairy industry for filtration of milk products such as whey. These membranes suffer from contamination caused by microorganisms present in milk during the filtration process. This biological contamination affects the life-time of the membrane and also filtration process itself. Thus, these membranes were modified with plasma treatment to incorporate amine functional groups for subsequent immobilization of silver and selenium nanoparticles to prevent the membranes from creation of biofilm of microorganisms. Antimicrobial activity of both types of nanoparticles and membranes with immobilized nanoparticles was determined against cell strains Staphylococcus aureus and Escherichia coli.
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Magnetické částice jako analytický nástroj v medicíně
Gablech, Evelína ; Pekárková, Jana (oponent) ; Hubálek, Jaromír (vedoucí práce)
Tato práce shrnuje poznatky o magnetických nanočásticích (MNPs), funkcionalizaci jejich povrchu, a jejich využití v různých oblastech. V literární rešerši popisuje využití magnetických naončástic v separačních technikách analytické biochemie pro separaci nukleových kyselin a proteinů. V experimentální části se zabývá syntézou MNPs metodou koprecipitace solí železa a následnou modifikací jejich povrchu. Při přípravě MNPs byl sledován vliv přítomnosti PEG v reakční směsi a vliv teploty na výsledné vlastnosti MNPs. Povrch MNPs byl modifikován dvěma různými metodami, metodou emulzní polymerace s výchozími látkami MMA a AAc, a metodou karboxylace pomocí 11- merkaptoundekanové kyseliny. PEG ani teplota neměli téměř žádný vliv na vlastnosti připravených MNPs. Navázání karboxylových skupin se podařilo pouze při použití metody emulzní polymerace.
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Semiconductive gas sensors for detection of organic gases and vapours
Šedivý, Matúš ; Gablech, Evelína (oponent) ; Gablech, Imrich (vedoucí práce)
This bachelor thesis is focused on gas sensors. Basic characteristics of sensors are discussed in the first part. Next part contains an overview of the most used types of gas sensors based on different physical principles. The main aim of thesis outlines last chapter where process of making gas sensors with different semiconductive metal oxides active layers is described. This thesis also describes measuring of their characteristics and characteristics of commercial gas sensor TGS 822 made by Figaro. Measured data are processed in graphs and tables and then were used for comparison with created sensors. In conclusion, there are discussed possibilities of improving selectivity of gas detection by using sensors with different active layer.
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Ion-selective membrane with antimicrobial effect
Gablech, Evelína ; Farka,, Zdeněk (oponent) ; Soukupová,, Jan (oponent) ; Drbohlavová, Jana (vedoucí práce)
This work deals with improvement of antimicrobial properties of commercial polymer membranes used in dairy industry for filtration of milk products such as whey. These membranes suffer from contamination caused by microorganisms present in milk during the filtration process. This biological contamination affects the life-time of the membrane and also filtration process itself. Thus, these membranes were modified with plasma treatment to incorporate amine functional groups for subsequent immobilization of silver and selenium nanoparticles to prevent the membranes from creation of biofilm of microorganisms. Antimicrobial activity of both types of nanoparticles and membranes with immobilized nanoparticles was determined against cell strains Staphylococcus aureus and Escherichia coli.
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Semiconductive gas sensors for detection of organic gases and vapours
Šedivý, Matúš ; Gablech, Evelína (oponent) ; Gablech, Imrich (vedoucí práce)
This bachelor thesis is focused on gas sensors. Basic characteristics of sensors are discussed in the first part. Next part contains an overview of the most used types of gas sensors based on different physical principles. The main aim of thesis outlines last chapter where process of making gas sensors with different semiconductive metal oxides active layers is described. This thesis also describes measuring of their characteristics and characteristics of commercial gas sensor TGS 822 made by Figaro. Measured data are processed in graphs and tables and then were used for comparison with created sensors. In conclusion, there are discussed possibilities of improving selectivity of gas detection by using sensors with different active layer.
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Magnetické částice jako analytický nástroj v medicíně
Gablech, Evelína ; Pekárková, Jana (oponent) ; Hubálek, Jaromír (vedoucí práce)
Tato práce shrnuje poznatky o magnetických nanočásticích (MNPs), funkcionalizaci jejich povrchu, a jejich využití v různých oblastech. V literární rešerši popisuje využití magnetických naončástic v separačních technikách analytické biochemie pro separaci nukleových kyselin a proteinů. V experimentální části se zabývá syntézou MNPs metodou koprecipitace solí železa a následnou modifikací jejich povrchu. Při přípravě MNPs byl sledován vliv přítomnosti PEG v reakční směsi a vliv teploty na výsledné vlastnosti MNPs. Povrch MNPs byl modifikován dvěma různými metodami, metodou emulzní polymerace s výchozími látkami MMA a AAc, a metodou karboxylace pomocí 11- merkaptoundekanové kyseliny. PEG ani teplota neměli téměř žádný vliv na vlastnosti připravených MNPs. Navázání karboxylových skupin se podařilo pouze při použití metody emulzní polymerace.
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Synthesis of spherical gold nanoparticles for biomedical applications
Gablech, Evelína ; Adam, Vojtěch (oponent) ; Drbohlavová, Jana (vedoucí práce)
This paper deals with synthesis of spherical gold nanoparticles for various biomedical applications. Gold nanoparticles were synthetized by green methods. Considerable part of this thesis is devoted to green procedures. The aim of this work was to synthetize stable colloid gold nanoparticles suitable for various biomedical applications; especially for in vivo and in vitro bioimaging techniques. Review of these methods is included in theoretical part of this thesis as well. A cytotoxicity test was also provided. Gold nanoparticles were characterized by methods SEM, DLS, UV-VIS.
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