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In vitro determination of antimicrobial, antioxidative and antiphlogistic aktivity of quinones from seeds of Nigella sativa
Kokoška, L. ; Maršík, Petr ; Landa, Přemysl ; Tesařová, H. ; Svobodová, B. ; Halamová, K. ; Flesar, J. ; Přibylová, Marie
The in vitro anti-yeast, antioxidant, and anti-inf1ammatory activities ofblack cumin (Nigella sativa) seed quinones, namely dithymoquinone (DTQ), thymohydroquinone (THQ), and thymoquinone (TQ), were evaluated in frame of this work. The results showed that THQ and TQ possessed significant anti-yeast activity and affected the growth of aH strains tested at two pH levels with minimum inhibitory concentrations ranging from 8 to 128 !lg/ml. The best scavenging activity was produced by THQ, which showed a remarkable activity of 2.60 Trolox equivalents (TE) in a concentration range between 1.6 and 6.4 !lg/mL and lCso value of 2.4 !lg/mL in ORAC and DPPH assays, respectively. Contrastingly, TQ possessed only weak DPPH scavenging efficacy (ICso = 170 !lg/mL) but significant antioxidative action of 1.91 TE in ORAC assay. In anti-inf1ammatory assay, THQ and TQ possessed significant inhibitory activity against COX-l and -2 at the concentrations comparable with indomethacin. The data presented in this work suggest that THQ and TQ are biologically effective agents with potential for application in food or phalmaceutical industries.