National Repository of Grey Literature 26 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
GENE EXPRESSION AND IMMUNOLOGICAL RESPONSE IN MICE EXPOSED TO ZnO NANOPARTICLES
Rössner ml., Pavel ; Vrbová, Kristýna ; Strapáčová, S. ; Rössnerová, Andrea ; Ambrož, Antonín ; Brzicová, Táňa ; Líbalová, Helena ; Javorková, Eliška ; Zajícová, Alena ; Holáň, Vladimír ; Kulich, P. ; Večeřa, Zbyněk ; Mikuška, Pavel ; Coufalík, Pavel ; Křůmal, Kamil ; Čapka, Lukáš ; Dočekal, Bohumil ; Šerý, Omar ; Machala, M. ; Topinka, Jan
We analyzed gene expression changes in the lungs and the immunological response in splenocytes of mice exposed by inhalation of ZnO nanoparticles - NP. Adult female ICR mice were treated for three days and three months, respectively. Analysis of differential expression in genes involved in oxidative stress was conducted using quantitative RT-PCR. The potential immunotoxic and immunomodulatory effects of ZnO NP were analyzed by phenotyping and cytokine production by splenocytes after three months exposure. Three days exposure resulted in down-regulation of GCLC, GSR, HMOX-1, NQO-1, NF-kB2, PTGS2 and TXNRD1 mRNA expression, three months exposure increased the expression of these genes. Three months exposure caused a significant decrease in the percentage of granulocytes in the spleen cells, and affected the production of IL-10 and IL-6 by lipopolysaccharide-stimulated leukocytes. In summary, our study revealed changes in the expression of genes involved in the oxidative stress response following acute ZnO NP exposure. Subchronic ZnO NP exposure induced immunomodulatory effects in the spleen.
Effect of inhaled metal oxide nanoparticles on organs of exposed mice
Mikuška, Pavel ; Večeřa, Zbyněk ; Dočekal, Bohumil ; Čapka, Lukáš ; Křůmal, Kamil ; Coufalík, Pavel ; Moravec, Pavel ; Buchtová, Marcela ; Míšek, Ivan ; Vrlíková, Lucie ; Smutná, Tereza ; Dumková, J. ; Hampl, A. ; Fictum, P.
Adult mice were continuously exposed to metal oxide nanoparticles for different time intervals. At the end of experiment, mice organs (lung, brain, kidney, liver and spleen) were collected for chemical, biochemical, histological and electron microscopical analyses.
Resistance of asphalt binder and asphalt mixture against deformation
Dubšíková, Karolína ; Coufalík, Pavel (referee) ; Dašek, Ondřej (advisor)
Subject of this bachelor´s thesis is to describe laboratory properties of asphalt binders in relation to permanent deformation. The principle and procedure of empirical tests needle penetration and softening point will be explained in the theoretical part. The principle and procedure of MSCR test will be also described. The properties of four asphalt binders will be evaluated in practical part. These binders will be used for production of asphalt mixtures, which will be subjected to wheel tracking test and their results will be compared.
Determination of levoglucosan and dehydroabietic acid in urban aerosol
Coufalík, Pavel ; Čmelík, Richard ; Křůmal, Kamil ; Čapka, Lukáš ; Mikuška, Pavel
The analytical method involving aerosol sampling into water, quantitative pre-concentration of levoglucosan and dehydroabietic acid, and their determination by LC-MS was developed in this work.
Rheological properties of asphalt binders
Coufalík, Pavel ; Bačová,, Katarína (referee) ; Komačka,, Jozef (referee) ; Hýzl, Petr (advisor)
Dissertation thesis is focused on rheological properties of asphalt binders and on changes of their performance during the lifetime in road structures. Aging processes of asphalt binders and methods of laboratory aging are described in the theoretical part of the thesis. Furthermore, basic rheological models and related test methods for the evaluation of asphalt binders are described. In the practical part, properties of asphalt binders are investigated after aging process and their performance is evaluated by empirical and functional test methods. The various methods of aging are compared with each other and a relationship between real conditions and laboratory aging is researched. In the next section, vacuum flashed cracked residue (VFCR) is added to the selected asphalt binders and their influence on rheological properties during description of aging of asphalt binders is investigated. The results were used in publication of two certified methodology under the title „The methodology for the evaluation of paving bitumen in terms of susceptibility to thermal oxidative aging“ and „The methodology describes laboratory aging of asphalt mixtures“. The results of the thesis are listed in the conclusion.
Dynamic viscosity of asphalt binders
Broďák, Marek ; Coufalík, Pavel (referee) ; Dašek, Ondřej (advisor)
The bachelor thesis describes a laboratory characteristics of bituminous binders. The principle, procedure of meansurment and comparison of test results of dynamic viscosity, needle penetration and softening point by ring and ball method will be described. The tests will be performed on samples of the bituminous binders with different degree of modification.
Determination of mercury and methylmercury in specific Antarctic soils in surroundings of seal carcasses
Zvěřina, O. ; Kuta, J. ; Coufalík, Pavel ; Komárek, J.
This work is dealing with the determination of total mercury and methylmercury in specific Antarctic soils, which occur in the surroundings of seal carcasses.
Determination of methylmercury in lichens and cyanobacterial mat by GC-AFS
Coufalík, Pavel ; Meszarosová, N. ; Coufalíková, K. ; Zvěřina, O. ; Komárek, J.
The aim of this research was a development of the analytical method suitable for the determination of trace concentration of methylmercury in the ecosystem of Antarctic lakes. The determination of mercury bioaccumulation in cyanobacterial mat (the colonies of algae and cyanobacteria) stands in focus as well.
Organic markers and compounds in PM1 aerosol in small town near Prague (Czech Republic) in winter 2016
Křůmal, Kamil ; Mikuška, Pavel ; Coufalík, Pavel ; Večeřa, Zbyněk
Contribution summarizes the concentrations of monosaccharide anhydrides, resin\nacids, methoxyphenols, monosaccharides, disaccharides, sugar alcohols, alkanes,\nhopanes, steranes and polycyclic aromatic hydrocarbons in PM1 aerosols in small town\nKladno-Švermov in winter. According to concentrations of organic markers, the\nidentification of emission sources in Kladno-Švermov is discussed.
Combined effect of temperature and CdO nanoparticles treatment on Picea abies
Večeřová, Kristýna ; Mikuška, Pavel ; Oravec, Michal ; Kozáčiková, Michaela ; Pompeiano, Antonio ; Coufalík, Pavel ; Urban, Otmar
Three-years old seedlings of Picea abies were pre-treated under two different\ntemperatures (20x35 °C) and subsequently exposed to elevated concentration of CdO\nnanoparticles (CdONPs). Two-week exposure to airborne CdONPs of ecologically\nrelevant size and concentration significantly reduced photosynthetic light use efficiency\nin plants as well as contents of photosynthetic pigments and saccharides. In contrary,\namino acid content was increased under CdONPs. These changes were further\nmodulated by previous growth temperature.

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1 Coufalík, Petr
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