National Repository of Grey Literature 28 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Use of Sodium Bicarbonate for Flue Gas Treatment in Small Scale.
Zach, Boleslav ; Pohořelý, Michael ; Šyc, Michal ; Svoboda, Karel ; Václavková, Šárka ; Moško, Jaroslav ; Brynda, Jiří ; Punčochář, Miroslav
The trend of reduction of landfilling can lead, especially in areas with low population density, to the need to build new waste-to-energy capacities in the form of small units. However, flue gas treatment in small scale has to be sufficiently simple to decrease capital costs and allow the construction of such facilities. For that reason, the possibility of one-step dry flue gas treatment at compromise conditions was investigated as well as the limitation of flue gas composition.\n\n
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Novel binding proteins derived from small protein domains targeting diagnostically important molecules
Vaňková, Lucie ; Malý, Petr (advisor) ; Brynda, Jiří (referee) ; Trefil, Pavel (referee)
The rapid development of the gene engineering techniques, especially methods for in vitro directed evolution and combinatorial mutagenesis, has triggered the generation of new binding agents to almost any antigen of interest as an alternative to broadly used antibodies. These so-called non-Ig scaffolds are often derived from proteins with useful biophysical properties. While the therapeutic market is still dominated by monoclonal antibodies, the easy option of desired customization of non-Ig binders by conventional methods of gene engineering predestine them largely for the use in the diagnostic area. The ABD scaffold, derived from a three-helix bundle of albumin-binding domain of streptococcal protein G, represents one of the small non-Ig scaffolds. In our laboratory, we have established a highly complex combinatorial library developed on the ABD scaffold. This ABD scaffold-derived library was used to generate unique binders of human prostate cancer (PCa) biomarkers PSP94, KLK2, KLK11 for the more precise diagnosis of PCa. The second part of the thesis describes the generation of ABD-derived binders selectively recognizing different phenotypes of circulating tumor cells as a binding component of the cell capture zone of microfluidic chip for lung adenocarcinoma diagnosis. Beside this already...
Glutamate Carboxypeptidase II - Structural and Biochemical Characterization and Structure-Assisted Drug Design
Ptáček, Jakub ; Bařinka, Cyril (advisor) ; Obšil, Tomáš (referee) ; Brynda, Jiří (referee)
Glutamate carboxypeptidase II (GCPII) is a human membrane-bound metallopeptidase discovered more than 30 years ago. It has attracted attention of biomedical scientists thanks to its diverse tissue expression profile and different biological functions. GCPII is detected on the surface of astrocytes in both central and peripheral nervous systems where it is responsible for the cleavage of N-acetyl-L-aspartyl-L-glutamate (NAAG), the most abundant mammalian peptidic neurotransmitter. Glutamate, one of the hydrolytic products, is a potent excitatory neurotransmitter and its overproduction has been shown to be responsible for cell death in various neurological disorders by a so-called glutamate excitotoxicity mechanism. Together with the fact that NAAG acts neuroprotectively it has been postulated (and later confirmed) that GCPII inhibition has a therapeutic potential in such disorders. Prostate cancer (PCa) is the second most prevalent cancer in men and despite its slow progression it is prone to metastasize thus posing a life threat. GCPII has been found to be overexpressed in prostate tumor cells compared to the healthy tissue (therefore it is also termed prostate-specific membrane antigen - PSMA) thus representing an excellent biomarker of PCa validated by many publications and clinical studies....
Properties of Biochar Produced by Slow Pyrolysis of Stabilized Sewage Sludge.
Moško, Jaroslav ; Pohořelý, Michael ; Skoblia, S. ; Beňo, Z. ; Brynda, Jiří ; Zach, Boleslav ; Šyc, Michal ; Václavková, Šárka ; Jeremiáš, Michal ; Svoboda, Karel
Direct application of stabilized sewage sludge on agricultural soil is currently a big concern because of its significant content of detectable organic micropollutants. Proper sludge treatment should suppress its negative features and produce material suitable for soil amendment. Slow pyrolysis is one of such treatments. We studied the influence of pyrolysis temperature (400-800 °C) on material and energy balances and on elementary composition of the pyrolysis solid residue (biochar). Pyrolysis at higher temperatures resulted in lesser biochar yield and promoted gas yield. The macronutrient content of biochar increased with an increase in pyrolysis temperature (except for nitrogen).
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Stabilized Sewage Sludge Pyrolysis: Properties of the Products.
Moško, Jaroslav ; Pohořelý, Michael ; Skoblia, S. ; Beňo, Z. ; Svoboda, Karel ; Václavková, Šárka ; Brynda, Jiří ; Larkhayeva, K. ; Zach, Boleslav ; Šyc, Michal
Příspěvek se zabývá materiálovou a energetickou bilancí pyrolýzy suchého stabilizovaného čistírenského kalu a základními vlastnostmi a složením pyrolýzních produktů.
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Structure Analysis of Plant Bifunctional Nuclease TBN1
Kovaľ, Tomáš ; Dohnálek, Jan (advisor) ; Brynda, Jiří (referee) ; Schneider, Bohdan (referee)
This work is dedicated to thorough structural analysis of plant bifunctional nuclease TBN1, the representative of plant nuclease I group. TBN1 along with homologous nucleases from this family plays an important role in plant cell life cycle and also shows considerable anticancerogenic effects. Two variants of TBN1 (wild type and N211D mutant) were studied. Properties of both variants in different solutions were analyzed. Both were successfully crystallized. Structures of both types of TBN1 were solved using X-ray diffraction. The phase problem was solved by Multi-wavelength anomalous dispersion using Zn2+ ions natively present in TBN1. Structural properties of TBN1 such as fold, active site composition, effect of glycosylation and surface electrostatic potential distribution were analyzed. Reaction mechanism of TBN1 was proposed on the basis of structural properties and comparisons with similar structures.

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1 Brynda, J.
1 Brynda, Jan
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