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Phase imaging below the diffraction limit
Nečesal, Daniel ; Bouchal, Zdeněk (oponent) ; Dvořák, Petr (vedoucí práce)
This Master's thesis is focus on construction of phase imaging systems capable of studying both micro- and nano-sized objects. The first chapter lays the foundation of optics and nano-photonics. Then the interference of coherent waves and its practical application will be discussed for the use of breaking the diffraction limit and extracting information out of optical systems. First experimental setup discussed will be the Mach--Zehnder type in--line digital holographic microscope. We show a way to construct this device out of cheap materials and how to design its control software. Then the experimental results created using this device are presented. In the last chapter, we focus on how a SNOM holography can be implemented, how it was built, and how automation via software was utilized to reduce operator time waste. Finally, we will present experimental results obtained from our system.
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Imaging of an object in turbid medium by combining the signal of ballistic and diffuse photons in the coherence-controlled holographic microscope
Ďuriš, Miroslav ; Bouchal, Zdeněk (oponent) ; Chmelík, Radim (vedoucí práce)
This thesis deals with the quantitative phase imaging (QPI) of objects behind scattering media in coherence-controlled holographic microscope (CCHM). It is possible to image in this microscope with completely incoherent illumination, which gives rise to the coherence-gating effect. It is a crucial property that allows the separation of ballistic and multiply scattered photons, therefore, a part of the thesis explains in depth the coherence-gate. Fundamentals of the image formation in CCHM are presented, which support many interpretations of results throughout the experimental part of the work. The goal of the thesis is to design a method for an observation of phase objects in turbid milieu and experimentally prove the method working. A novel method based on the preliminary research and the analytical derivation is proposed. It is based on an acquisition of multiple images for different reference field shifts. Each shift corresponds to the imaging by a different group of photons. The particular QPIs formed by ballistic or scattered photons can be superposed to create synthetic image of improved quality. Experiments with variously complex samples provided information about limitations of this method.
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Phase imaging below the diffraction limit
Nečesal, Daniel ; Bouchal, Zdeněk (oponent) ; Dvořák, Petr (vedoucí práce)
This Master's thesis is focus on construction of phase imaging systems capable of studying both micro- and nano-sized objects. The first chapter lays the foundation of optics and nano-photonics. Then the interference of coherent waves and its practical application will be discussed for the use of breaking the diffraction limit and extracting information out of optical systems. First experimental setup discussed will be the Mach--Zehnder type in--line digital holographic microscope. We show a way to construct this device out of cheap materials and how to design its control software. Then the experimental results created using this device are presented. In the last chapter, we focus on how a SNOM holography can be implemented, how it was built, and how automation via software was utilized to reduce operator time waste. Finally, we will present experimental results obtained from our system.
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Imaging of an object in turbid medium by combining the signal of ballistic and diffuse photons in the coherence-controlled holographic microscope
Ďuriš, Miroslav ; Bouchal, Zdeněk (oponent) ; Chmelík, Radim (vedoucí práce)
This thesis deals with the quantitative phase imaging (QPI) of objects behind scattering media in coherence-controlled holographic microscope (CCHM). It is possible to image in this microscope with completely incoherent illumination, which gives rise to the coherence-gating effect. It is a crucial property that allows the separation of ballistic and multiply scattered photons, therefore, a part of the thesis explains in depth the coherence-gate. Fundamentals of the image formation in CCHM are presented, which support many interpretations of results throughout the experimental part of the work. The goal of the thesis is to design a method for an observation of phase objects in turbid milieu and experimentally prove the method working. A novel method based on the preliminary research and the analytical derivation is proposed. It is based on an acquisition of multiple images for different reference field shifts. Each shift corresponds to the imaging by a different group of photons. The particular QPIs formed by ballistic or scattered photons can be superposed to create synthetic image of improved quality. Experiments with variously complex samples provided information about limitations of this method.
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