National Repository of Grey Literature 8 records found  Search took 0.00 seconds. 
Different response of maize inbred and hybrid genotypes to drought: analysis of leaf proteome and photosynthetic processes
Benešová, Monika
Drought is one of the most important stress factors affecting plants. Increasing drought resistance via plant breeding is currently the main approach for improvement of plant productivity and reduction of water usage. Plant breeders and scientists search for reliable and easy methods of the determination of drought sensitivity in different genotypes. A precise knowledge about processes underlying plant stress response and defence against limited water availability is necessary for this. The aim of this study was a detailed analysis of photosynthetic processes and changes in leaf proteome (again aimed especially at proteins involved in photosynthesis) during drought stress. This analysis was a part of a complex project focused on the possible causes of genetic variability and drought resistance in agronomically important crops. Another goal was to analyse whether the measurement of selected photosynthetic parameters can be used for the determination of drought sensitivity per se and/or for the prediction of the response of hybrids based on known behaviour of their parents. Young plants of maize inbred lines and their hybrids were used as a model for this analysis, which was made under conditions of moderate and more severe drought. Two inbred lines which were (together with their F1 hybrids) used...
Different response of maize inbred and hybrid genotypes to drought: analysis of leaf proteome and photosynthetic processes
Benešová, Monika ; Holá, Dana (advisor) ; Prášil, Ilja (referee) ; Pospíšilová, Jana (referee)
Drought is one of the most important stress factors affecting plants. Increasing drought resistance via plant breeding is currently the main approach for improvement of plant productivity and reduction of water usage. Plant breeders and scientists search for reliable and easy methods of the determination of drought sensitivity in different genotypes. A precise knowledge about processes underlying plant stress response and defence against limited water availability is necessary for this. The aim of this study was a detailed analysis of photosynthetic processes and changes in leaf proteome (again aimed especially at proteins involved in photosynthesis) during drought stress. This analysis was a part of a complex project focused on the possible causes of genetic variability and drought resistance in agronomically important crops. Another goal was to analyse whether the measurement of selected photosynthetic parameters can be used for the determination of drought sensitivity per se and/or for the prediction of the response of hybrids based on known behaviour of their parents. Young plants of maize inbred lines and their hybrids were used as a model for this analysis, which was made under conditions of moderate and more severe drought. Two inbred lines which were (together with their F1 hybrids) used...
The Changes of Body Temperature of Patients during a Medical Procedure in Total Anesthesia
BENEŠOVÁ, Monika
The first part of the present dissertation shows mechanisms of keeping of body temperature in an optimum range as well as it divides and evaluates range of body temperature. My dissertation also deals with the ways of measurement of body temperature, methods of measurement and tools which are neccessary for measurement of body temperature. Another part presents characteristics of aneshtesia its division, ways of application and tools which are used for total anesthesia. It is also described an observation of body temperature during total anesthesia, negative consequences of hypothermia, regulations of body temperature during total anesthesia and prevention and treatment of hypothermia. There are descriptions of targets and hypothesis in the second part. There were made five hypothesis. The third part shows methods of collecting data itself. They are results of stucture observation of changes of body temperature of patiens in total anesthesia during surgery.The fourth part are charts and graphs showing results of measurements of body temperature of our patients in total anesthesia as well as results of the questionnaires based on answers from asked nurses. There are particular hypothesis and their results in discussion part. Hypothesis H1 was proved. Hyphothesis H2 is not possible to be disproved. Hyphothesis H3 was not proved. Hyphothesis H4 was proved and hyphotesis H5 was proved. We can deduce from the results that active use of tools for keeping body temperature in a physiological range can prevent decrease of body temperature. It is clear from the questionnaires that nurses are aware of complications connected to hypothermia. They actively use tools for keeping body temperature in physiological range. There is a summary in the conclusion of the dessertation whether all estimated targets were accomplished. As a result of the dissertation we can assume that using of tools for keeping body temperature of total anasthesia patients should be a standard. There should be also a standard to measure body temperature during an operation in total anasthesia itself.
In vitro expansion and activation of natural killer cells for cell therapy.
BENEŠOVÁ, Monika
NK cells are part of the non-specific immune response and are one of the main components of antitumor immunity. They do not need antigen stimul for thein activation but recognize damaged ( transformed ) cells by characteristic decreased expression of MHC I molecules. These natural killers become subjekt of many clinical studies based on the use of anti-tumor activity of NK cells for both solid tumors and in hemato - oncological diseases. The aim of this study was to find optimal conditions for in vitro expansion and activation of NK cells. NK cells were isolated from mononuclear cell fraction by magnetic separation and cultured in two types of media SCGM and X - VIVO 10 with the addition of interleukin-2 respectively OKT3 antibody. The influence of the mononuclear cells on proliferation of NK cells was tested. After a 6- day culture , the cells were passaged and growth of NK cells was determined using hematological analyzer and flow cytometry. Expression of the activation markers CD25 and CD336 (NKp44) was observed. All experiments were conducted under conditions of good manufacturing practice. Slightly higher gain of NK cells was observed in SCGM media without a significant differences in the other additives. Much higher number of NK cells was observed in culture with supporting irradiated mononuclear cells. There were no differences in cultures with autologous or allogeneic cells. We found that NK cells with higher proliferative potential express increasingly CD25, unlike the cells with decreased proliferation which had increased expression of CD336 marker. The work led to the definition of the optimal culture conditions for NK cells and became the basis for the further development of the medicinal product from in vitro activated NK cells.

See also: similar author names
1 BENEŠOVÁ, Marcela
10 BENEŠOVÁ, Marie
11 BENEŠOVÁ, Markéta
12 BENEŠOVÁ, Martina
8 BENEŠOVÁ, Michaela
7 BENEŠOVÁ, Monika
1 Benešová, M.
2 Benešová, Magdaléna
10 Benešová, Marie
11 Benešová, Markéta
12 Benešová, Martina
8 Benešová, Michaela
2 Benešová, Michala
2 Benešová, Milena
3 Benešová, Miroslava
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