National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Effect of selected cytostatics for the treatment of leukemia on the activity of human carbonyl reducing enzymes
Šmídlová, Monika ; Novotná, Eva (advisor) ; Wsól, Vladimír (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical sciences Candidate: Bc. Monika Šmídlová Supervisor: RNDr. Eva Novotná, Ph.D. Title of diploma thesis: Effect of selected cytostatics for the treatment of leukemia on the activity of human carbonyl reducing enzymes Key words: reductases, leukemia, cytostatics, inhibition Anthracycline antibiotics, especially daunorubicin, are widely used for the treatment of acute myeloid leukemia (AML) and acute lymphocytic leukemia (ALL). Although the efficacy of these drugs is high, treatment is still limited due to cardiotoxicity and tumor cell resistance to anthracyclines. Mechanisms that contribute to the formation of anthracycline resistance include metabolic biotransformation (reduction) to less efficient secondary alcohols. The reduction is calatyzed by carbonyl reducing enzymes belonging to aldo-keto reductase (AKR) and short chain dehydrogenase/reductase (SDR) superfamilies. The discovery of AKR and SDR inhibitors could help to overcome anthracycline resistance and also reduce cardiotoxicity caused by these drugs. The aim of the diploma thesis was to find out whether all-trans-retinoic acid, cyclophosphamide, cytarabine, cladribine and prednisolone are able to inhibit anthracycline reductases AKR1A1, AKR1B10, AKR1C3, AKR7A2...
Effect of selected cytostatics for the treatment of leukemia on the activity of human carbonyl reducing enzymes
Šmídlová, Monika ; Novotná, Eva (advisor) ; Wsól, Vladimír (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical sciences Candidate: Bc. Monika Šmídlová Supervisor: RNDr. Eva Novotná, Ph.D. Title of diploma thesis: Effect of selected cytostatics for the treatment of leukemia on the activity of human carbonyl reducing enzymes Key words: reductases, leukemia, cytostatics, inhibition Anthracycline antibiotics, especially daunorubicin, are widely used for the treatment of acute myeloid leukemia (AML) and acute lymphocytic leukemia (ALL). Although the efficacy of these drugs is high, treatment is still limited due to cardiotoxicity and tumor cell resistance to anthracyclines. Mechanisms that contribute to the formation of anthracycline resistance include metabolic biotransformation (reduction) to less efficient secondary alcohols. The reduction is calatyzed by carbonyl reducing enzymes belonging to aldo-keto reductase (AKR) and short chain dehydrogenase/reductase (SDR) superfamilies. The discovery of AKR and SDR inhibitors could help to overcome anthracycline resistance and also reduce cardiotoxicity caused by these drugs. The aim of the diploma thesis was to find out whether all-trans-retinoic acid, cyclophosphamide, cytarabine, cladribine and prednisolone are able to inhibit anthracycline reductases AKR1A1, AKR1B10, AKR1C3, AKR7A2...
Current possibilities of laboratory diagnostics of anemia
Šmídlová, Monika ; Vrbacký, Filip (advisor) ; Pešková, Eliška (referee)
Author: Monika Šmídlová Title: Current possibilities of laboratory diagnostics of anemia Type of assignment: Bachelor's thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Branch of study: Healthcare Laboratory Assistant (ZDLB) This bachelor's thesis discusses the general informations and summary of etiopathogenetical classification of anaemia and possibilities of laboratory diagnostics. Thesis is mainly focused on laboratory diagnosis of inherited types of anaemia. Anaemia or anaemia syndrome is a pathological condition characterized by low levels of haemoglobin and hematocrit under the physiological limit. Clinical manifestations are variously severe depending on development of disease and type of anaemia. Anaemia patient suffer from reduced oxygenation of tissues which determinates many symptoms of tissue hypoxia. Other symptoms are associated with hypovolemia and hyperkinetic circulation that can occur. In haemolytic anaemia, patients have symptoms of intravascular or extravascular haemolysis. Primary (hereditary) anaemia are congenital, the cause lies in genetic mutations that result a changes of phenotype properties of erythrocytes. The process of diagnosis determinating includes correct identification of family history, relevant medical examinations and laboratory...

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