National Repository of Grey Literature 5 records found  Search took 0.00 seconds. 
Efficacy overflows
Šlechtová, Tereza ; Úterský, Michal (referee) ; Hluštík, Petr (advisor)
The aim of this bachelor´s thesis is to process the recherche of the overflow chambers The first part of this thesis includes the legislation of the overflow chambers. The following list provides a short description of the individual types of the overflow chambers and building materials which we use the most often. The thesis deals with technical proposal and hydraulic computation.The allovable pollution of diluted water is presented by dint of emission limits and pollution limits which are caused by the overflow chambers. In the practical part of this thesis are used valuable lessons learned from the theoretical parts. The aim of this part is recognition of the selected overflow chambers in the combined sewerage system in the city of Brno. The chambers were monitoring during measuring campaign in 2017. The overflow chambers were evaluated to their efficiency and construction conditions. In the latest bulit and current moved overflow chambers was calculated the ratio of dilution.
Study of peptide digestion kinetics by trypsin and chiral separations of biologically active compounds by HPLC
Šlechtová, Tereza ; Tesařová, Eva (advisor) ; Čabala, Radomír (referee) ; Mikšík, Ivan (referee)
This dissertation thesis composes of two parts; the first part focus on the characterization of trypsin, enzyme frequently used in proteomic research for the investigation and identification of protein sequences, and its peptide digestion kinetics. The second part is aimed to the enantioseparations of biologically active compounds. First part of this project focus on tryptic digestion of synthetic peptides and the development of HPLC method for the identification of synthetic peptides and their fragments. Using the in-solution digestion and HPLC method, relative kinetic constants were determined for problematic sequences. Amino acids responsible for the decrease in trypsin catalytic activity and their location towards the cleavage site were studied. Certain slight exopeptidase activity of trypsin was noted, especially at the end of peptide chain. Furthermore, three columns with immobilized trypsin used in HPLC were compared concerning their catalytic activity. The immobilization of enzymes on solid support is used to elevate the amount of enzyme present during digestion and to assure better repeatability and reproducibility of obtained results. Activity of a new trypsin column synthesized at the University of North Carolina at Chapel Hill was compared to two commercially available trypsin columns....
Comparison of methods for detection and determination of enantiomers of theanine in HPLC
Šlechtová, Tereza ; Tesařová, Eva (advisor) ; Bosáková, Zuzana (referee)
This thesis focused on optimization of separation methods for underivatized theanine enantiomers on two teicoplanin-based chiral stationary phases and separation methods for enantiomers derivatized by 9-fluorenylmethyloxycarbonyl chloride and dansylchloride. Using these optimized conditions the limits of detection and quantification were calculated. Under optimized conditions were also investigated the contents of theanine enantiomers in green tea and in selected dietary supplement containing L-theanine.
Determination of pesticides enantiomers in water
Šlechtová, Tereza ; Lokajová, Jana (referee) ; Tesařová, Eva (advisor)
This Barchelor's project deals with chiral herbicide mecoprop and its determination by high-performance liquid chromatography method with teicoplanin based chiral stationary phase. The teoretical part focus on basic facts conserning chirality, chiral pesticides and problems related to their usage and effects to the enviroment. It also involves the ways of their determination by the above mentioned method and gives several examples of previous separations. The experimental part is based on method optimalization and determination of changes in mecoprop enantiomeric ratio in various water samples during different storage conditions.
HPLC separation of chlorophenoxypropionic acid derivatives
Horová, Kateřina ; Kalíková, Květa (advisor) ; Šlechtová, Tereza (referee)
This Bachelor's thesis focuses on the separation of chlorophenoxypropionic acids and their derivatives by a high-performance liquid chromatography. The separations were performed in a reversed phase mode. These compounds are employed as herbicides, i.e. chemical substances to control unwanted plants, to kill weeds. The herbicides studied for the purposes of this thesis were 2-(4-chloro-2-methylphenoxy)propionic acid, 2-(2,4- dichlorophenoxy)propionic acid, 2-(2-chlorophenoxy)propionic acid, 2-(3- chlorophenoxy)propionic acid and 2-(4-chlorophenoxy)propionic acid. The study aimed at finding and optimizing chromatographic conditions for simultaneous separation of all five herbicides mentioned above. The analysis employed two types of columns, that is ZORBAX SB-C8 column and ZORBAX Eclipse XDB-C18 column whose level of polarity differ. As mobile phases, there were used acetonitrile/water, acetonitrile/formic acid, pH 2.1 and methanol/formic acid, pH 2.1 in various volume ratios. ZORBAX SB-C8 column, mobile phase methanol/formic acid (concentration of 0.365 mol/l, pH 2.1) 40/60 (v/v), temperature of 25řC, and flow rate of 1 ml/min delivered the most convenient separation conditions. Given these isocratic conditions, all the herbicides were baseline separated within 80 minutes. Keywords: HPLC,...

Interested in being notified about new results for this query?
Subscribe to the RSS feed.