National Repository of Grey Literature 22 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Study of the posttrans lation al modifications of phosducin
Šimůnek, Jiří ; Obšil, Tomáš (advisor) ; Alblová, Miroslava (referee)
The aim of this diploma thesis was to study the role of posttranslational modifications of phosducin and their role in the interaction with the 14-3-3 protein as well as the influence of the complex formation on these modifications. Phosducin is a 33kDa protein commonly present in photoreceptor cells of the retina as well as other tissues. Despite many experiments, its physiological functions are still elusive. It has been speculated that fosducin plays an important regulatory role in visual phototransduction pathway, regulation of blood pressure and expression of G-proteins. The phosducin function is regulated through binding to the 14-3-3 protein, a regulatory protein involved in many biochemical processes. Phosducins binding to 14-3-3 protein requires phosphorylation of two serine residues Ser-54 and Ser-73 within the N-terminal domain of phosducin. However, the role of the 14-3-3 protein binding in the regulation of phosducin function is still unclear. In this diploma thesis proteins 14-3-3ζ∆C and phosducin (mutation Q52K) were successfully expressed and purified. The effect of the complex formation on phosducin posttranslational modifications was investigated using limited proteolysis and dephosphorylation. These experiments revealed that the complex formation significantly slowed down both...
Study of interaction between phosducin and the 14-3-3 protein
Šimůnek, Jiří ; Obšil, Tomáš (advisor) ; Alblová, Miroslava (referee)
The main goal of this bachelor thesis is to study the interaction between phosducin and the 14-3-3 protein which are involved in the regulation of visual signal transduction in vertebrate retina. This process is mediated by G-protein signaling pathway and is regulated by several proteins including phosducin depending on light intensity. Phosducin, a 33kDa protein, is expressed in many tissues mainly in photoreceptor cells of the retina. The visual signal transduction and its amplification are regulated through inhibition of the G-protein (transducin) function. The signal transduction involves a dissociation of heterotrimeric transducin (Gtαβγ) to the α subunit (Gtα) and the βγ complex (Gtβγ). The signalling is terminated by their reassociation back to the Gtαβγ complex. The phosducin binds to Gtβγ and thus prevents the formation of the Gtαβγ heterotrimer. This decreases the amount of functional Gtαβγ complexes and suppresses the signal transduction. If the signal transduction needs to be amplified (e.g. during the night) then phosducin is phosphorylated and this inhibits its interaction with Gtβγ. The phosphorylated phosducin is bound to the 14-3-3 protein. However, the role of the 14-3-3 protein in the regulation of phosducin is still unclear. The 14-3-3 protein is a 28kDa protein which is...
Role of Magnetosheath Parameters on Magnetopause Processes
Tkachenko, Oksana ; Šafránková, Jana (advisor) ; Kulhánek, Petr (referee) ; Šimůnek, Jiří (referee)
Title: Role of magnetosheath parameters on magnetopause processes Author: Oksana Tkachenko Department: Department of Surface and Plasma Science Supervisor: Prof. RNDr. Jana Šafránková, DrSc. e-mail address: Abstract: The thesis addresses of two regions significant for the solar wind- magnetosphere coupling: the cusp in high geomagnetic latitudes and the low-latitude (LLBL) subsolar magnetopause. A penetration of plasma of a solar origin into the magnetosphere could be realized directly through magnetospheric cusps. The region adjacent to the magnetopause in the cusp vicinity is highly turbulent, occupied by the heated magnetosheath- -like plasma with a low drift velocity for which occurrence of vortices is a very common feature. In the first part of the thesis, we present a de- tailed analysis of a vortex-like structure created by a turbulent plasma flow around the magnetopause indentation above the cusp and using the data from Interball-1 and Magion-4; we find necessary conditions and a possible mechanisms to creation of such structures. The second part concerns the low-latitude boundary layer formation, its spatial structure and temporal changes based on THEMIS multipoint observations. In spite of its cru- cial role in transfer of mass, momentum, and energy from the...

National Repository of Grey Literature : 22 records found   1 - 10nextend  jump to record:
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2 Šimůnek, Jan
16 Šimůnek, Jiří
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