National Repository of Grey Literature 23 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Lease of real estates from the perspective of mandatory payments
Šimůnek, Jiří ; Marková, Hana (advisor) ; Boháč, Radim (referee)
1 Název diplomové práce, abstrakt a klíčová slova v anglickém jazyce Title of the thesis: Lease of real estates from the perspective of mandatory payments Abstract The aim of this diploma thesis was mainly to describe the mandatory payments connected with the lease of real estates according to the legal regulation of the Czech Republic. The thesis works with the term lease defined by the author for the purposes of this writing not in relation to the contract type but to the extent of the resulting license to use the real estates. Mandatory payments are identified in the strict sense of the term (personal and corporate income tax and value added tax), public insurance (social security and public health insurance) and local fees relevant to the lease of real estate. In this thesis, the institutes of lease, accommodation / temporary lease borrowing, tenure, informal lease, servitude, chief rent and right of building are described in detail from the civil law point of view and they are assigned specific tax obligations. In terms of absolute property rights, the institutes of pledge and lien and the management of foreign assets, including the trust fund, do not meet the established definition of lease of real estates. A separate chapter discusses the phenomenon of shared economy in the area of short-term...
Study of the posttrans lation al modifications of phosducin
Šimůnek, Jiří ; Obšil, Tomáš (advisor) ; Alblová, Miroslava (referee)
The aim of this diploma thesis was to study the role of posttranslational modifications of phosducin and their role in the interaction with the 14-3-3 protein as well as the influence of the complex formation on these modifications. Phosducin is a 33kDa protein commonly present in photoreceptor cells of the retina as well as other tissues. Despite many experiments, its physiological functions are still elusive. It has been speculated that fosducin plays an important regulatory role in visual phototransduction pathway, regulation of blood pressure and expression of G-proteins. The phosducin function is regulated through binding to the 14-3-3 protein, a regulatory protein involved in many biochemical processes. Phosducins binding to 14-3-3 protein requires phosphorylation of two serine residues Ser-54 and Ser-73 within the N-terminal domain of phosducin. However, the role of the 14-3-3 protein binding in the regulation of phosducin function is still unclear. In this diploma thesis proteins 14-3-3ζ∆C and phosducin (mutation Q52K) were successfully expressed and purified. The effect of the complex formation on phosducin posttranslational modifications was investigated using limited proteolysis and dephosphorylation. These experiments revealed that the complex formation significantly slowed down both...
Study of interaction between phosducin and the 14-3-3 protein
Šimůnek, Jiří ; Obšil, Tomáš (advisor) ; Alblová, Miroslava (referee)
The main goal of this bachelor thesis is to study the interaction between phosducin and the 14-3-3 protein which are involved in the regulation of visual signal transduction in vertebrate retina. This process is mediated by G-protein signaling pathway and is regulated by several proteins including phosducin depending on light intensity. Phosducin, a 33kDa protein, is expressed in many tissues mainly in photoreceptor cells of the retina. The visual signal transduction and its amplification are regulated through inhibition of the G-protein (transducin) function. The signal transduction involves a dissociation of heterotrimeric transducin (Gtαβγ) to the α subunit (Gtα) and the βγ complex (Gtβγ). The signalling is terminated by their reassociation back to the Gtαβγ complex. The phosducin binds to Gtβγ and thus prevents the formation of the Gtαβγ heterotrimer. This decreases the amount of functional Gtαβγ complexes and suppresses the signal transduction. If the signal transduction needs to be amplified (e.g. during the night) then phosducin is phosphorylated and this inhibits its interaction with Gtβγ. The phosphorylated phosducin is bound to the 14-3-3 protein. However, the role of the 14-3-3 protein in the regulation of phosducin is still unclear. The 14-3-3 protein is a 28kDa protein which is...

National Repository of Grey Literature : 23 records found   1 - 10nextend  jump to record:
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2 ŠIMŮNEK, Jan
2 Šimůnek, Jan
17 Šimůnek, Jiří
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