National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Gene mutations affecting the development of human testes
Macháčová, Simona ; Šebková, Nataša (advisor) ; Ševčíková Jonáková, Jana (referee)
Development of mammalian sex organs is remarkable process. Both ovaries and testes rise from the same precursor but then differentiate into morphologically and functionally different structure. The determining factors that decide the fate of undifferentiated structures are not just the genes themselves but also the timing of transcription regulatory genes and specific amount of their products. Development of male and female gonads manages a large set of regulatory genes that interact with each other. Together it forms the gene regulatory network. Crucial role in male sex differentiation plays an SRY gene which regulates the time and quantity of the other factors expression. Even slight errors (mutations) in genes sequences of regulatory genes could change their expression. This leads to disruption of the development of sex organs or even death of the whole organism. Naturally occurring mutations and their phenotype in humans are suitable for studies of gene sex regulatory factors. Thanks to these studies we are able to detect the cause of many birth defects what is a first step toward their eventual treatment.
Description of Xenopus tropicalis pre-Sertoli cells in tissue culture
Ševčíková Jonáková, Jana ; Tlapáková, Tereza (advisor) ; Drda Morávková, Alena (referee)
5 Abstract Xenopus tropicalis is an important model organism used in developmental and cell biology. Long term primary mixed cell culture, derived from juvenile testis of Xenopus tropicalis, is the first tissue culture of this specie. Characterization of this tissue culture is important for its further handling and subsequent experiments. The culture is composed of Sertoli cell precursors. Feeder layer of this cells enables long term germinal stem cell cultivation. Influence of condicioned medium plays important role during the cell culture cultivation. Result of this task approved effect of condicioned medium for cell culture growth. Cell culture and testis gene expression analyse revealed, that in process of Sertoli cell differentiation many growth and differentiation factors are involved. Presence of marker genes has been approved by RT-PCR and in situ hybridization. This in vitro system can contribute to clarification of different roles of pre-Sertoli and Sertoli cells in testis differentiation, and therefore it establishes a new spermatogenesis model offering the possibility to distinguish parts of the pre-Sertoli and Sertoli cells differentiation, which is difficult in mammal models. Keywords: Xenopus tropicalis, tissue culture, pre-Sertoli cells, Sertoli cells, testicular differentiation, gene expression

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