National Repository of Grey Literature 72 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
The functional in vitro analysis of the BRCA1alternative splicing variants
Ševčík, Jan ; Kleibl, Zdeněk (advisor) ; Stopka, Tomáš (referee) ; Macůrek, Libor (referee)
BACKGROUND: The inactivation of the tumor suppressor gene BRCA1 is a predisposing factor for a breast/ovarian cancer development. Formation of cancer-specific alternative splicing variants with aberrant biological properties can represent additional mechanism decreasing the overall BRCA1 activity in DNA double strand break (DDSB) repair. In this study, we analyzed BRCA1 alternative splicing variants BRCA114-15 and 17-19 ascertained previously during the screening of high-risk breast cancer individuals. METHODS: We established a stable MCF-7 cell line-based model system for an in vitro analysis of BRCA1 variants. Using this system, we analyzed the impact of BRCA114-15 and 17-19 variants on DNA repair kinetics using comet assay and confocal immunomicroscopy. The capacity of DNA repair was assessed directly by an in vitro NHEJ assay and indirectly by a mitomycin C sensitivity test. The proliferation activities were determined by a clonogenic assay and growth curves. RESULTS: Overexpression of BRCA114-15 and 17-19 increases the endogenous level of DNA damage, slows down the DDSB repair, and decelerates the initial phase of radiation-induced foci formation and prolongs their persistence. Moreover, BRCA114-15 and 17-19 differentially influence the activity of HR and NHEJ and sensitivity of MCF-7 cells to ionizing...
Design and manufacture of bending fixture
Ševčík, Jan ; Trčka, Tomáš (referee) ; Zemčík, Oskar (advisor)
The work deals with the design and manufacture of bending fixture. It contains a comparsion with current bending fixtures on the market and a justification for the production of a given type. Then the work contains a proposal of the constuction of individual parts with the selection and description of technologies of their production and the process of their production. The work also contains complete drawing ducumentation of all parts of the bending fixture. The conclusion of the work contains the possibilities of design modifications and economic and technological evaluation.
Analýza stavu ekologického zemědělství a bioprodukce ve vybraném regionu Zlínského kraje - okres KROMĚŘÍŽ
Ševčík, Jan
The diploma thesis „Analysis of the conditions of the ecological agriculture and bioproduction in the selected region Zlín, Kroměříž district deals with the contemporary conditions of the ecological agriculture and bioproduction in the Kroměříž district and with the availability of a range of goods for consumers. Principals and goals of the ecological agriculture, legislation, control mechanisms and production of the organic food are described in the literature review. History and development of the eco-agriculture in both the Czech Republic and the world are also described here. Next section focuses on the description of the Kroměříž district and its environmental conditions for agriculture as well as of the contemporary conditions of the eco-agriculture and production of the organic food. Last part focuses on the analysis of the companies in which the overall benefits for the region is found out through a questionnaire survey. The second questionnaire will be targeting directly on consumers of the organic food. The research findings and subsequent discussion are summarised in the conclusion.
Robotizované dojení a jeho působení na dojnice
Ševčík, Jiří
The objective of the dissertation thesis was to evaluate the effects of two different types of milking systems (CMS - conventional milking system vs. AMS - an automatic milking system) on average daily milk yield, on frequency of attending robots, on pre-milking teat preparation procedures and stimulation and milking time, on the teats traumatization of Holstein cows. The experiments were performed in two locations: Žabčice School Farm of Mendel University in Brno and ZEMAX, a. s. in Šitbořice. Data was collected for a period 1st January 2014 to 15th December 2015. Higher average milk yield per day and lower milk yield per milking on the other hand on the farm with AMS were caused by a higher frequency of milking. Cows on farm with CMS were milked twice a day. On the farm with AMS dairy cows were milked in accordance with their own needs. Milking frequency in the AMS was in 2014 2.47, in 2015 2.88 per day. Heat stress has negative impacts on milk production. Effect of heat stress showed more markedly in 2015, which is probably related to the fact that the value of THI greater than 80 lasted longer. The length of pre-milking teat preparation procedures and stimulation various neighborhoods was found in AMS in 2014 from 0:13 min. to 0:16 min., in 2015 from 0:14 min. to 0:19 min. On farm with CMS time not be registered. The length of milking time with CMS in both years was 5:02 min. In AMS was in 2014 from 3:03 minutes to 3:53 min., in 2015 from 3:05 min. to 4:45 minutes. In process of milking machine teat traumatization were smaller when using AMS.
Draft Procurement Logistics Management in a Manufacturing Company
Zouhar, Jan ; Ševčík, Jan (referee) ; Jurová, Marie (advisor)
The dissertation aims on evaluating of logistic processes at chosen company, which is ITAB Shop Concept CZ, a.s. Based on data analysis, suggestions leading to improvement of logistics related processes are established to secure continuous and economical way of acquiring supplies required for production and sales.
Sanitary technical installations and gas pipeline in the hotel
Ševčík, Jan ; Wierzbická, Helena (referee) ; Vaščáková, Alena (advisor)
Diploma thesis deals with design of sanitary and gas instalation in hotel building. It is hotel with four floors and basement. Theteoretical part is focused on grey water treatment systems and possibility of their use in the building. The Computational part and project includes design of sanitary and stormwater sewer, water supply system, gas installations a their service pipes to current utility lines.
Technologies of reuse of wastewater in place of need
Podhrázský, Martin ; Ševčík, Jan (referee) ; Hlavínek, Petr (advisor)
Final thesis is focusing on possibilities hidden in the potential of wastewaters in sewers. The goal of this thesis is to present technologies and experiences with water reclamation and with heat recovery systems using wastewaters as a source of heat energy. The theoretical part is divided in two main topics. The first topic is focused on bringing important knowledge and experiences with designing and operating decentralized sewer mining systems, which are, due to less favourable environmental conditions, largely applicated and developed in countries such as Australia, California, etc. The second topic is focusing on heat recovery from sewage water and possibilities of heating and cooling buildings. A possibility of using heat energy from sewage for heating and cooling a swimming pool building located in Brno, Czech Republic was assessed in the last part of this thesis. As a source of wastewater was considered a sewer main located near the pool building. Payback periods and economical effectivity of investing into wastewater heat recovery system was compared with centralized heat supply system, which is a very common source of heat energy in Czech Republic.
The importance of microbiome composition and activity on homeostasis of the organism (maintaining health)
Turek, B. ; Šíma, Petr ; Ševčík, J. ; Kajaba, I.
Naše studie se zabývá střevním mikrobiomem a jeho přímým a nepřímým vlivem na celkové zdraví.
Clinical classification of sequence variants in non-coding regulatory regions in breast cancer susceptibility genes.
Bubáková, Eliška ; Ševčík, Jan (advisor) ; Vodička, Pavel (referee)
Inactivation of tumor supressor gene BRCA1 causes a life-long risk of breast carcinoma development. Genetic screenings of indicated individuals from high-risk families help to identify large number of sequence variants in known predisposing genes. Majority of discovered variants doesn't have clinical significance yet which causes a big problem for diagnostics. Some of these variants are found within regulatory non-coding regions of gene. A part of the clinical classification of variants is their functional characterization. The goal of this thesis was to create a model system for functional characterization of variants in non-coding regions and to verify its function. Model system was based on targeted gene manipulation by co-transfecting CRISPR-Cas9 construct and donor construct that contained a portion of BRCA1 gene sequence with analyzed modifications, into U2 OS cells. The cells have stably integrated DR-GFP system which allows the activity of homologous recombination (HR) to be determined. Monoallelic modifications were induced into U2 OS cells. These modifications were in a Kozak sequence region of BRCA1 gene. Expression level of BRCA1 mRNA was determined by qRT-PCR, which showed the same levels of mRNA in all cells with analyzed alterations. Next, expression level of BRCA1 protein was...

National Repository of Grey Literature : 72 records found   1 - 10nextend  jump to record:
See also: similar author names
6 Ševčík, Jakub
22 Ševčík, Jan
7 Ševčík, Jaroslav
2 Ševčík, Jiří
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