|
|
Tailoring of cooling procedure during freeze-casting for bone replacement applications.
Šantavý, Tomáš ; Novotná, Lenka (oponent) ; Salamon, David (vedoucí práce)
Bachelor thesis focuses on preparation of hydroxyapatite ceramics by freeze-casting. Hydroxyapatite is an important ceramic material, due to its biocompatibility, biodegradability and bioactivity it has a wide range of use in medicinal applications especially in replacement of bone material. The chosen method, freeze-casting, is flexible, eco-friendly and we are able to achieve a high level of porosity. The method includes freezing, freeze drying and sintering which creates a porous structure of the prepared material. Objective of this bachelor thesis was to find out the impact on the created porosity and mechanical properties of the final material. Freezing rates were applied 3, 4, 5, 6, 7 and 26 m/s. During the experimental part it was established that the freezing rate has a significant influence on the newly formed inner microstructure, it directly influences the interlamellar distances and mechanical property which is compressive strength. With liquid nitrogen with a speed of 26 m/s the achieved compressive strength was of 15 MPa as well as the shortest interlamellar distances of 14-22 µm. With slower freezing rate the average interlamellar distances were 40-150 µm and compressive strength of 2-4 MPa with comparable porosity.
|
|
|
Influence of selected types of food on changes in the composition of the intestinal microbiome
Šantavý, Tomáš ; Němcová, Andrea (oponent) ; Ryšávka, Petr (vedoucí práce)
The master's thesis focuses on determining the influence of selected types of food on changes in the intestinal microbiome composition. Dietary composition plays an important role in controlling intestinal microbial populations, including the prevention and treatment of certain diseases. In order to evaluate the effect of daily consumption of fruit-vegetable smoothie on the human intestinal microbiome, changes in the intestinal microbiome at the beginning and after 14 days of intervention were compared. The experimental part was attended by 10 men and 10 women aged 35-45 years, who were evenly divided into 2 groups. The first group of participants consumed a combination of orange, dried turmeric and ginger. The second group consumed a combination of apple, carrot and lemon juice. DNA isolation from stool samples followed by a quantitative PCR method was used to detect differences in the intestinal microbiome. After 14 days of consumption of the fruit-vegetable smoothie, specific changes in the phylum Firmicutes, Bacteroidetes and the genus Lactobacillus, Bifidobacterium were observed. After statistical evaluation of the obtained results, the differences were not detectable at a statistically significant level (p-value > 0.05).
|
|
|
Tailoring of cooling procedure during freeze-casting for bone replacement applications.
Šantavý, Tomáš ; Novotná, Lenka (oponent) ; Salamon, David (vedoucí práce)
Bachelor thesis focuses on preparation of hydroxyapatite ceramics by freeze-casting. Hydroxyapatite is an important ceramic material, due to its biocompatibility, biodegradability and bioactivity it has a wide range of use in medicinal applications especially in replacement of bone material. The chosen method, freeze-casting, is flexible, eco-friendly and we are able to achieve a high level of porosity. The method includes freezing, freeze drying and sintering which creates a porous structure of the prepared material. Objective of this bachelor thesis was to find out the impact on the created porosity and mechanical properties of the final material. Freezing rates were applied 3, 4, 5, 6, 7 and 26 m/s. During the experimental part it was established that the freezing rate has a significant influence on the newly formed inner microstructure, it directly influences the interlamellar distances and mechanical property which is compressive strength. With liquid nitrogen with a speed of 26 m/s the achieved compressive strength was of 15 MPa as well as the shortest interlamellar distances of 14-22 µm. With slower freezing rate the average interlamellar distances were 40-150 µm and compressive strength of 2-4 MPa with comparable porosity.
|
host ::
RSS.