Národní úložiště šedé literatury Nalezeno 12 záznamů.  1 - 10další  přejít na záznam: Hledání trvalo 0.02 vteřin. 
Multi-cationic aminopyrene-based tag for oligosaccharide analysis by capillary electrophoresis with laser-induced fluorescence detection
Smolková, Denisa ; Duša, Filip ; Čmelík, Richard ; Lavická, Jana
We present further optimization of the labeling method with a new multi-cationic aminopyrene-based tag, APTMP (4,4’,4’’-(8-aminopyrene-1,3,6-trisulfonyl)tris(1-methylpiperazine) in order to achieve higher resolution and sensitivity of CE/LIF analysis of APTMP labeled oligosaccharides.
Hexahistidine-based tag for capillary electrophoresis-mass spektrometry analysis of oligosaccharides and N-linked glycans
Partyka, Jan ; Křenková, Jana ; Čmelík, Richard ; Foret, František
Various basic amino acids and peptides were studied as an alternative labels for capillary electrophoresis-mass spectrometry analysis of oligosaccharides. The hexahistidine-based label with big number of positive charges allowed to use effective and sensitive CE-MS analysis in positive ion mode both neutral and acidic glycans.
Synthetic approaches for new labeling tags facilitating glycan analysis
Čmelík, Richard ; Křenková, Jana ; Foret, František
This study is focused on the synthesis of labels based on the pyrene core bearing several ionizable quaternary amines and the efficient carbonyl-reactive group, such as primary amine or hydrazine.
SMV-2017-743: Preparation, testing and delivery of isoelectric point standards
Čmelík, Richard
The project continues long-term cooperation of both participants. The low-molecular mass pI markers were synthesized and examined with aim to fill up and deliver the previously described compounds.
SMV-2017-732: Preparation, testing and delivery of isoelectric point standards
Čmelík, Richard
The project continues long-term cooperation of both participants. The low-molecular mass pI markers were synthesized and examined with aim to fill up and deliver the previously described compounds.
Stanovení levoglukosanu a dehydroabietové kyseliny v městském aerosolu
Coufalík, Pavel ; Čmelík, Richard ; Křůmal, Kamil ; Čapka, Lukáš ; Mikuška, Pavel
Cílem této práce byl vývoj analytické metody pro stanovení levoglukosanu a dehydroabietové kyseliny v aerosolu pomocí LC-MS s využitím přímého vzorkování aerosolu do vody.
Evaluation of stationary phases for HPLC based on nanofibers
Čmelík, Richard ; Foret, František
In this study, the performance parameters were compared for the set of chromatographic columns filled with polyacrylonitrile nanofibers processed in the different manner. The advantages and challenges of the novel fillings were discussed.
Determination of levoglucosan in urban aerosol
Coufalík, Pavel ; Mikuška, Pavel ; Čmelík, Richard
The main aim of this work was a development of the analytical method for levoglucosan\ndetermination including direct collection of aerosol into water phase, preconcentration,\nand determination by LC-MS.
Study of the cell wall of Staphylococcus aureus and its sensitivity to enzybiotics
Čmelík, Richard ; Melková, K. ; Kobzová, Š. ; Janda, L.
The endolysin resistant and sensitive strains of Staphylococcus aureus were compared by means of LC-MS based structural analysis of peptidoglycan isolated from their cell walls. The structural explanation of the resistance was suggested.
Analysis of saccharides in grapes and wine using HPLC with various stationary phases
Flodrová, Dana ; Čmelík, Richard ; Šalplachta, Jiří ; Bobálová, Janette
The quality of wine is influenced by its saccharides content, especially glucose and fructose. Monitoring of wine oligosaccharides is of great interest, because of their physiological, medical and food importance. The aim of study was to develop method for fast analysis of saccharide composition of wine, grape juice and grapes.

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