National Repository of Grey Literature 213 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Dřevěné brýlové obruby design a výroba
Černý, Jan
Main task of the thesis is the design of new models of eyeglasses from a wood composite, including the production of physical prototypes. The work is divided into three main categories. The introductory part focuses on general requirements and standards for spectacle frames. The second part focuses on the improvement of technological processes in the manufacture of wooden glasses and the third one, the practical part is dedicated to the proces of designig, including the analysis of the current design of wooden frames and their accessories for sale and to the production of their own prototypes. The work is accompanied by continuous photos from the glasses production process.
The role of protein tyrosine phosphatase CD45 and Src-family kinases in murine model of chronic autoinflammatory osteomyelitis
Ilievová, Kristýna ; Brdička, Tomáš (advisor) ; Černý, Jan (referee)
The development of autoinflammatory diseases is caused by the dysregulation of innate immune mechanisms. This leads to the development of spontaneous inflammation. Mice lacking adaptor protein PSTPIP2 develop chronic autoinflammatory osteomyelitis due to higher activity of neutrophil granulocytes and their increased production of IL-1β. .β. PSTPIP2 interacts with PEST phosphatases and kinase CSK. These proteins are impor- tant negative regulators of Src family kinases. In this diploma thesis, the role of Src family kinases and the role of their positive regulator phosphatase CD45 in the development of chronic autoinflammatory osteomyelitis was studied. For this purpose, a mouse model of chronic autoinflammatory osteomyelitis (CMO) lacking CD45 was used. These mice deve- lop the disease with delayed kinetics. Bone marrow cells isolated from these mice produce less IL-1β. upon silica activation and have lower phosphorylation of ERK MAP kinase. It isβ. probably caused by higher phosphorylation of the inhibitory tyrosine of Src family kinases resulting in their lower activity. The presence of different immune cell populations in the bone marrow, spleen and blood of these mice was also monitored in these mice. The re- sults of this work contribute to a better understanding of the role of Src family...
Study of the materno-fetal microchimerism of the APC using MHCII/EGFP mouse model and clearing histological techniques
Knížková, Karolina ; Černý, Jan (advisor) ; Schwarzer, Martin (referee)
Microchimerism arises from the exchange of cells between genetically distinct individuals. The coexistence of genetically distinct cell populations within a single organism has possible effects on health and functioning of individuals immune systems, but the exact mechanisms of action are often not yet known. With the development of microscopic technologies and software for data analysis, the possibilities of detection and phenotyping of these rare cell populations are expanding. My intention in this work is to find maternal microchimerism in embryonic tissues (E13) and intestines of breastfed pups using MHCII/EGFP knock-in mouse model. Several different technologies potentially suitable for the detection of maternal microchimeric cells in offspring tissues (light sheet fluorescent microscopy - LSFM, virtual slide microscopy and flow cytometry) were selected. Advanced analysis of the obtained samples from the light sheet microscopy using the creation of a neural network was used here. The presence of maternal microchimerism was not demonstrated by flow cytometry. Using LSFM, image data were obtained from intestinal samples of suckling pups, which were processed by the neural network method. Data analysis of embryos (E13) obtained by the same method did not allow data analysis due to high...
Mitochondrial tumour suppressors
Jakoubě, Pavel ; Kečkéšová, Zuzana (advisor) ; Černý, Jan (referee)
Cancer is one of the most feared diseases in our modern society and many resources are spent on developing new ways of diagnosis, prevention and treatment. Luckily for us, our bodies already have a first line of defence against carcinogenesis - proteins called tumour suppressors. Studying these proteins can give us an important insight into the inner workings of this disease and can also show us new ways of combating it. One of the signs of cancer cells is dysregulation of metabolic processes and since mitochondria play a pivotal part in many of these processes, we wanted to research the identity and role of mitochondrial tumour suppressors. Indeed, several such tumour suppressors have been identified, having a plethora of functions such as modulating the activity of other mitochondrial enzymes, directly participating in cellular metabolic pathways, affecting reactive oxygen species production and modulating hypoxia-induced signalling. The focus of this work is to gather the available information about these important protective proteins. Key words: cancer, tumour suppressor, SIRT3, SIRT4, POX/PRODH, MTUS1/MTSG1, FUS1/TUSC2, LACTB, FH, SDH, mitochondrial
Protein quality control in the secretory pathway of eukaryotic cells
Bařinková, Markéta ; Stříšovský, Kvido (advisor) ; Černý, Jan (referee)
More than 30 % of the cellular proteome enters the secretory pathway during biogenesis in eukaryotic cells. The secretory pathway then ensures that these proteins are correctly folded, undergo necessary post- translational modifications, and reach their target site in membrane organelles or outside of the cell. Since a significant number of the nascent proteins in the pathway are or become dysfunctional, the cell must possess quality control mechanisms by which to weed them out. As proteins travel through the secretory pathway they may be degraded by various pathways in the endoplasmic reticulum, Golgi apparatus, endosomes, or at the plasma membrane. These degradatory pathways utilize a number of molecules including chaperones, ubiquitin ligases, and many others. They are coordinated by a unifying principle - the unfolded protein response, which acts as a support mechanism in case the degradation pathways are overwhelmed. The study of protein quality control mechanisms is necessary as they help us understand the production of a significant portion of the cellular proteome. Furthermore, defects in these degradation pathways are linked to several human diseases such as cystic fibrosis or some neurodegenerative diseases. These protein degradation pathways have been studied for decades, but thanks to...
Regulation of leukocyte signal transduction by membrane adaptor proteins and kinases
Borna, Šimon ; Brdička, Tomáš (advisor) ; Černý, Jan (referee) ; Mejstříková, Ester (referee)
Signaling pathways must be finely tuned to assign a signal of appropriate strength and duration to the receptor stimulation. Their dysregulation can be very harmful. The consequences of dysregulated signaling pathways vary from autoimmunity, immunodeficiency, and autoinflammation to abnormal proliferation and cancer. In my thesis I aimed to characterize the roles of kinases and membrane associated or transmembrane adaptor proteins in signaling pathways downstream of different receptors. First, I was comparing the roles of SRC family kinases (SFK) in the initiation of antigen receptor signaling in B cells and in T cells. This effort resulted in the manuscript where we re-evaluated current data, which suggested that SYK can initiate BCR signaling independently of SFK. We show that much lower SFK activity is required for the initiation of BCR signaling than for TCR signaling, but we did not find any evidence for SFK-independent signal transduction. We also found that multiple factors are responsible for setting the higher threshold for SFK activity required to initiate signaling by TCR, including differences between SYK and ZAP-70, structure of the antigen receptor itself and separation of the receptor from transmembrane adaptor LAT, which is a major hub coordinating the formation of TCR signalosome....
Bioinformatical analysis of the complex multidimensional microscopy datasets
Backová, Lenka ; Černý, Jan (advisor) ; Čapek, Martin (referee)
Microscopy is embedded in the history of life sciences and vice versa. Recent advances in the field present new challenges as new revolutionary technologies arise. Sample prepa- ration, microscope operation and data analysis have become particularly demanding re- quiring specific interdisciplinary expertise. Bioimaging data analysis is computationally demanding, as microscopy technologies can easily acquire data of exceptional size, often in terabytes. Correct analysis requires computer vision knowledge, as well as knowledge of studied biological systems and last, but not least deep understanding of microscopy technology. Tools available for the analysis of the imaging data vary from open-source customizable software with a coverage of multiple tasks to a task specific proprietary software. To choose the best tools for the analysis, analysts should know their options and tasks at hand. In bioimage analysis the tasks needed to be employed depend on the desired outcome and the acquisition technology. Amongst the possible tasks to con- sider belong deconvolution, segmentation and registration. Amount of approaches and algorithms available is progressively growing, resulting in a complex field, difficult to be easily familiar with. My thesis covers different microscopy technologies with emphasis on...
CX3CR1+ migratory dendritic cells in the mechanisms of central tolerance
Březina, Jiří ; Filipp, Dominik (advisor) ; Černý, Jan (referee)
Display of thousands of self-antigens in the thymus is fundamental for the establishment of central tolerance as its failure can lead to the development of autoimmunity. Medullary thymic epithelial cells (mTECs) and thymic dendritic cells (DCs) constitute essential populations of antigen presenting cells (APCs) which present these self-antigens to developing T cells. While mTECs produce and present antigens in self-autonomous manner, DCs can hijack mTEC-derived antigens by the process of cooperative antigen transfer (CAT). It is well found that CAT is essential for working central tolerance, however, the overall heterogeneity of thymic APCs participating in CAT remains unclear. Using transgenic mouse models and multicolor flow cytometry analysis, we determined that APCs involved in CAT are exclusively of CD11c+ phenotype. Within these cells, we identified previously unrecognized CX3CR1+ subset of migratory DCs (mDCs) exhibiting monocyte/macrophage markers. These CX3CR1+ mDCs are more efficient in CAT than their CX3CR1- counterparts and reveal robust antigen presenting properties with the capability to present CAT-acquired antigen. Genetic ablation of CX3CR1+ mDCs resulted in increased cellularity of CD8+ and CD4+ thymocytes, indicating importance of this mDC subset for negative selection of...
Interaction between hydrogenosomes and endoplasmic reticulum in Trichomonas vaginalis
Kučerová, Jitka ; Tachezy, Jan (advisor) ; Černý, Jan (referee)
Endoplasmic reticulum-mitochondria encounter structure (ERMES) is a protein complex tethering ER and mitochondria. ERMES consists of four core subunits - Mmm1, Mmm2 (Mdm34), Mdm10 and Mdm12. It was first discovered in Saccharomyces cerevisiae and most functional information is based on studies of this organism. ERMES affects mitochondrial distribution and morphology, participates in lipid trafficking and is important for homeostasis of the cell. In Trichomonas vaginalis, the human urogenital parasite, three genes for putative, highly divergent components of ERMES complex were predicted. However, the cell localization of these proteins and their function is unknown. This thesis is focused on investigation of ERMES components in T. vaginalis, their cellular localization, interactions between components and identification of their possible interacting partners.
Exploring novel strategies targeting HBV
Šmilauerová, Kristýna ; Grantz Šašková, Klára (advisor) ; Černý, Jan (referee)
An effective and safe vaccine against Hepatitis B virus already exists, yet morbidity and mortality of this illness are still high. The key to developing a reliable treatment is a deep knowledge of the virus' life cycle and functions of all its components. In the presented work we explored an interactome of the Core protein of the Hepatitis B virus. Using proximity-dependent biotin identification technique (BioID) coupled to mass spectrometry we have identified a list of potential candidates that are either significantly enriched (in total 105 proteins) or less abundant in the presence of the HBV Core protein in the cell (40 proteins). The list also includes known HBV Core interacting proteins SRPK1 and SRPK2, and p53 protein whose expression is known to be repressed due to the HBV Core interaction with the E2F1 transcription factor. Many of the newly identified possible HBV Core interacting proteins are involved in biological processes already known or are suspected to be influenced by the HBV such as translational and transporting processes or gene expression and macromolecule production. Overall, this work comprehensively characterizes the interaction landscape of the HBV Core protein in the live cells and might thus serve as a reliable start for in depth HBV-host interaction analysis. Key...

National Repository of Grey Literature : 213 records found   1 - 10nextend  jump to record:
See also: similar author names
53 ČERNÝ, Jan
4 ČERNÝ, Jaroslav
27 ČERNÝ, Jiří
3 ČERNÝ, Josef
35 Černý, Jakub
1 Černý, Jan Bc.
1 Černý, Jan Karel
2 Černý, Jan,
1 Černý, Jaromír
4 Černý, Jaroslav
3 Černý, Jindřich
27 Černý, Jiří
3 Černý, Josef
2 Černý, Jáchym
53 Černý, Ján
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