National Repository of Grey Literature 11 records found  1 - 10next  jump to record: Search took 0.00 seconds. 
GENE EXPRESSION AND IMMUNOLOGICAL RESPONSE IN MICE EXPOSED TO ZnO NANOPARTICLES
Rössner ml., Pavel ; Vrbová, Kristýna ; Strapáčová, S. ; Rössnerová, Andrea ; Ambrož, Antonín ; Brzicová, Táňa ; Líbalová, Helena ; Javorková, Eliška ; Zajícová, Alena ; Holáň, Vladimír ; Kulich, P. ; Večeřa, Zbyněk ; Mikuška, Pavel ; Coufalík, Pavel ; Křůmal, Kamil ; Čapka, Lukáš ; Dočekal, Bohumil ; Šerý, Omar ; Machala, M. ; Topinka, Jan
We analyzed gene expression changes in the lungs and the immunological response in splenocytes of mice exposed by inhalation of ZnO nanoparticles - NP. Adult female ICR mice were treated for three days and three months, respectively. Analysis of differential expression in genes involved in oxidative stress was conducted using quantitative RT-PCR. The potential immunotoxic and immunomodulatory effects of ZnO NP were analyzed by phenotyping and cytokine production by splenocytes after three months exposure. Three days exposure resulted in down-regulation of GCLC, GSR, HMOX-1, NQO-1, NF-kB2, PTGS2 and TXNRD1 mRNA expression, three months exposure increased the expression of these genes. Three months exposure caused a significant decrease in the percentage of granulocytes in the spleen cells, and affected the production of IL-10 and IL-6 by lipopolysaccharide-stimulated leukocytes. In summary, our study revealed changes in the expression of genes involved in the oxidative stress response following acute ZnO NP exposure. Subchronic ZnO NP exposure induced immunomodulatory effects in the spleen.
Effect of inhaled metal oxide nanoparticles on organs of exposed mice
Mikuška, Pavel ; Večeřa, Zbyněk ; Dočekal, Bohumil ; Čapka, Lukáš ; Křůmal, Kamil ; Coufalík, Pavel ; Moravec, Pavel ; Buchtová, Marcela ; Míšek, Ivan ; Vrlíková, Lucie ; Smutná, Tereza ; Dumková, J. ; Hampl, A. ; Fictum, P.
Adult mice were continuously exposed to metal oxide nanoparticles for different time intervals. At the end of experiment, mice organs (lung, brain, kidney, liver and spleen) were collected for chemical, biochemical, histological and electron microscopical analyses.
Determination of dicarboxylic acids in air by aerosol sampler
Čapka, Lukáš ; Mikuška, Pavel
Dicarboxylic acids (DCAs) are important components of water soluble aerosol compounds. Aerosol particles are quantitatively collected using a continous aerosol sampler, CGU-ACTJU, into deionized water. The ACTJU effluent is permanently sucked out from the sampler and on-line analysed for DCAs content by means of ion chromatography.
Determination of levoglucosan and dehydroabietic acid in urban aerosol
Coufalík, Pavel ; Čmelík, Richard ; Křůmal, Kamil ; Čapka, Lukáš ; Mikuška, Pavel
The analytical method involving aerosol sampling into water, quantitative pre-concentration of levoglucosan and dehydroabietic acid, and their determination by LC-MS was developed in this work.
Determination of inorganic anions in air by modernized aerosol sampler
Čapka, Lukáš ; Mikuška, Pavel ; Večeřa, Zbyněk
Combination of original ACTJU sampler with water-based condensation growth unit (CGU) located upstream of the ACTJU allows quantitative sampling of aerosol particles down to a few nm in diameter. The ACTJU effluent is permanently sucked out from the sampler and online analysed for particulate water-soluble species by ion chromatography.
Increase of efficiency of continuous aerosol sampler
Mikuška, Pavel ; Čapka, Lukáš ; Večeřa, Zbyněk
Contribution deals with increasing of collection efficiency of ACTJU sampler also for particles smaller than 300 nm in diameter. Use of so-called „growth tube“ (GT) located upstream of the ACTJU results in increase of particle size to several µm. Enlarged particles are then easily quantitative collected in ACTJU sampler.
Determination of dicarboxylic acids in atmospheric aerosols using ion chromatography with on-line connected preconcentration unit
Čapka, Lukáš ; Mikuška, Pavel ; Večeřa, Zbyněk
A method for the fast and sensitive detection of dicarboxylic acids in atmospheric\naerosols was optimized. The method is based on the online sampling and subsequent\nanalysis with ion chromatography. The system was applied to the determination of\nDCAs in urban aerosols in Brno. The method was verified using a laboratory generated\nstandard aerosol of DCAs. A sampling of aerosol particles on parallel filter was used\nas a reference method.
Use of Capillary Zone Electrophoresis for Determination of Selected Analgetics in Water
Čapka, Lukáš ; Opatřilová, Radka (referee) ; Márová, Ivana (referee) ; Vávrová, Milada (advisor)
From viewpoint of environmental analysis in the whole world became popular in the latest years the question of drugs’ breakthrough to the component of environment. These contami-nants belong to the biological active compounds, with different physical-chemical and biolog-ical properties and evince great tendency to bioaccumulation. They penetrate to the environ-ment because of their increasing of usage and wrong techniques of liquidation. The most often use drugs are preparations against pain – analgetics, and from this category there are non-steroidal anti-inflammatory drugs. The frequent usage of this compounds relate to their easy accessibility. From this large group of compounds was chosen for monitoring: diclofenac, ibuprofen, ketoprofen, salicylic acid, naproxen and acetaminophen; because they include in favorite preparations. The monitoring matrix was the wastewater from two waste water treat-ment plants (WWTP). The sampling was performed in inflow and outflow because the com-paring of concentration of selected contaminants and discovering of efficiency of removing the polutants reliance on treatment technology. For extraction of selected contaminants was used solid phase extraction (SPE) and for determination was used capillary zone electrophore-sis (CZE) with diode array detection (DAD). There was identified and quantified all of se-lected analgetics in inflow and so in outflow of WWTP. That means, this polutants infuse into surface water and then into other components of environment.
Application of spectrophotometry for determination of drug residua in waters
Čapka, Lukáš ; Čáslavský, Josef (referee) ; Zlámalová Gargošová, Helena (advisor)
Residua of pharmaceuticals represent biologically active compounds which can interfere with some purification processes in WWTP. Control laboratories of many WWTP and water works are equipped with UV-VIS spectrophotometers. Therefore, the main goal of this thesis was the elaboration of the method for the determination of selected drugs in water based on the VIS spectrophotometry.
System for fast analysis of explosives in the enviromnemt
Čapka, Lukáš ; Večeřa, Zbyněk ; Mikuška, Pavel ; Šesták, Jozef ; Kahle, Vladislav
Detection of explosives is the subject of many investigations by law enforcement agents and forensic scientist. The identification and discrimination of evidence taken from crime scenes is a common practice in forensic investigations. The higher demands on simple and fast determination of hazard materials or strange objects are required in connection with worldwide often repeated terrorist activities.

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1 Čapka, Ladislav
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